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High efficiency production of ginsenoside compound K by catalyzing ginsenoside Rb1 using snailase

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Chinese Journal of Chemical Engineering 2018年第7期26卷 1591-1597页

作者： Zhiguang Duan Chenhui Zhu Jingjing Shi Daidi Fan Jianjun Deng Rongzhan Fu Rong Huang Cuiying Fan Shaanxi Key Laboratory of Degradable Biomedical Materials School of Chemical EngineeringNorthwest UniversityTaibai North Road 229Xi'an 710069China Shanxi R&D Center of Biomaterials and Fermentation Engineering School of Chemical Engineering Northwest University Taibai North Road 229Xi'an 710069China

The rare ginsenoside Compound K （C-K） is attracting more attention because of its good physiological activity and urgent need. There are many pathways to obtain ginsenoside C-K, including chemical and biological methods. Among these, the conversion of PPD-type ginsenosides by enzymatic hydrolysis is a trend due to its high efficiency and mild conditions. For effectively extracting from the other panaxadiol saponins, the conversion process for ginsenoside C-K was investigated using snailases in this study. The univariate experimental design and response surface methodology were used to determine the optimal hydrolysis conditions for the conversion of ginsenoside Rbl into ginsenoside C-K by snailases. The optimum conditions were as follows： pH 5,12, temperature 51 ℃, ratio of snailase/substrate 0.21, and reaction time 48 h. On the basis of these parameters, the addition of 1.0 mmol· L- 1 ferric ion was found to significantly improve the enzymolysis ofsnailases for the first time. With the above conditions, the maximum conversion rate reached 89.7%, suggesting that the process can obviously increase the yield of ginsenoside C-K. The bioassay tests indicated that the ginsenoside C-K showed anti-tumor activity in a series of tumor cell lines. Based on these results, we can conclude that the process of rare ginsenoside C- K production by enzymolysis with snailase is feasible, efficient, and suitable for the industrial production and application.

关键词： Ginsenoside Rb1 Ginsenoside Compound K Snailase enzymolysis

Production of Free Amino Acid and Short Peptide Fertilizer from Rapeseed Meal Fermentation Using Bacillus flexus NJNPD41 for Promoting Plant Growth

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Pedosphere 2018年第2期28卷 261-268页

作者： LIU Hongjun ZHONG Xi HUANG Yan QIAO Cece SHAO Cheng LI Rong SHEN Qirong Jiangsu Provincial Key Lab for Solid Organic Waste Utilization Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization National Engineering Research Center for Organic-based Fertilizers Nanjing Agricultural University

Free amino acids and short peptides(FAPS)that play an important role in plant nutrition can be extracted from rapeseed meal(RSM)by microbial *** enzymolytic activity of one bacterial strain isolated from RSM for the production of FAPS fertilizer(FAPSF)via solid-state fermentation,as well as its detoxification activity for isothiocyanates and oxazolidinethione,was investigated in this *** strain NJNPD41 isolated from RSM piled-soil possessed effective proteolytic activity and was identified as Bacillus flexus on the basis of its morphological,physiological,and biochemical properties,as well as 16S rDNA *** to the uninoculated control,inoculation with the strain NJNPD41 significantly increased the yield of total free amino acids and short peptides by 115%and decreased the content of isothiocyanates and oxazolidinethione by 53%and 60%,respectively,after solid-state *** pot and field experiments showed that FAPSF significantly promoted eggplant growth and enhanced the fruit yield and quality of eggplant compared to the *** conclusion,this study provided a novel method for the high-value utilization of RSM to produce high-quality and low-toxicity FAPSF using the newly isolated strain *** NJNPD41.

关键词： bacterial strain detoxification activity isothiocyanate microbial enzymolysis N pool organic fertilizer solid-statefermentation oxazolidinethione

Arsenic Removal from Pinctada martensii Enzymatic Hydrolysate by Using Zr(Ⅳ)-Loaded Chelating Resin

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Journal of Ocean University of China 2013年第3期12卷 392-396页

作者： YANG Xiaoman DAI Wenjin SUN Huili PAN Jianyu Yantai Institute of Coastal Zone Research Chinese Academy of Sciences South China Sea Institute of Oceanology Chinese Academy of SciencesCAS Key Laboratory of Marine Bio-Resourses Sustainable Utilization University of Chinese Academy of Sciences

The present study investigated the removal of inorganic arsenic from Pinctada martensii enzymatic hydrolysate through unmodified resin （D296） and Zr（IV）-loaded chelating resin （Zr-D401）. By loading Zr to macroporous chelating resin D401, the as exchange adsorption active sites are generated. This transforms D401 from a material that does not have the arsenic adsorption capacity into a material that has excellent arsenic exchange adsorption capacity. The static adsorption experiments were conducted to investigate the optimal removal condition for D296 and Zr-D401. The experimental results show that： the optimum condition for D296 is that T= 25℃, pH= 5, resin additive amount= 1 g （50 mL）-1, and contact time = 10 h, the corresponding arsenic removal rate being 65.7%, and protein loss being 2.33%; the optimum condition for Zr-D401 is that T=25 ℃, pH = 8, resin additive amount= 1 g （50 mL）-1, and contact time=10 h, the corresponding arsenic removal rate being 70.3%, and protein loss being 4.65%. These results show that both of the two resins are effective in arsenic removal for preserving useful substance. Our research provides scientific evidence and advances in the processing technology for heavy metal removal in shellfish.

关键词： Pinctada martensii enzymolysis arsenic removal chelating resin

A Method of Purification of Bovine Colostrum sIgA

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Journal of Northeast Agricultural University(English Edition) 2008年第2期15卷 23-25页

作者： LIU Xiaofei GAO Xuejun YAO Yonghao ZHOU Shenghua Key Laboratory of Dairy Science of Ministry of Education Northeast Agricultural University Harbin 150030 China Harbin Kanpure Dairy Limited Company Harbin 150000 China College of Veterinary Medicine Northeast Agricultural University Harbin 150030 China

sIgA in bovine colostrum was purified by ultrafiltration and enzymolysis methods in this experiment, and the prepared substance was detected by Western Blot. The purity and yield were up to 73.6% and 65.2%, respective... 详细信息

关键词： bovine colostrum sIgA purification enzymolysis ultrafiltration

Study on Biotransformation of Traditional Chinese Herbal Medicines Complex

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China Detergent & Cosmetics 2018年第4期3卷 45-49页

作者： Feng Chunbo Qiao Xiaoling Cao Ping Shanghai Jahwa United Co. Ltd.

A newly designed enzymolysis-fermentation combined method to dramatically enhance the actives level and skin benefits of traditional Chinese herbs (TCHs) was developed, biotransformation process under optimal reaction conditions can significantly transform molecular structures and obtained fermentation extracts can deliver better skin benefits on anti-aging, hydration and whitening. Analytical results showed that the ginsenoside Rg3, total sugar, polyphenols and flavonoids in fermented extract were 2~4 times higher than unfermented extract. In-vitro tests including DPPH radical scavenging activity, tyrosinase inhibition, cells proliferation and Hyaluronan-CD44 activity, have showed significant enhancement of efficacy, inhibition rate on DPPH antioxidation as example achieved over 60% improvement, which makes traditional Chinese herbs application more feasible. It is inferred that this study potentially enables the herbs to deliver required bioefficacy in cosmetic application.

关键词： enzymolysis fermentation biotransformation traditional Chinese herbs

Enzymatic pretreatment and microwave extraction of asiaticoside from Centella asiatica

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Journal of Biomedical Science and Engineering 2009年第7期2卷 526-531页

作者： Cheng-Xiao Wang Wei Han Liang Fan Chun-Li Wang 不详

The extraction of asiaticoside from Centella asiatica by enzymatic pretreatment and microwave extraction (EPME) was studied in this article. The effects of several important factors such as temperature of enzymatic pretreatment, liquid to solid ratio and microwave radiation time were investigated by quadric regression orthogonal design experiment and were analyzed by response surface. An extraction model with well forecast performance was then established. The results indicate that the optimum extraction condition was as follows: liquid to solid ratio was 36mL/g, temperature of enzymatic pretreatment was 45℃, enzymatic time was 30min, and microwave ra-diation time was

关键词： Asiaticoside enzymolysis Microwave Extraction Centella Asiatica

Effects of Different Wall-breaking Methods on Extraction of Solanesol from Stems and Leaves of Potato

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Agricultural Biotechnology 2017年第2期6卷 11-14页

作者： Bingpeng WANG Jing MENG Mei GUO Xinyi HUANG Fangyuan DONG Duolong DI Department of Pharmacy Gansu University of Medicine/Key Laboratory of Chemistry and Quality for Traditional Chinese Medicines of the College of Gansu ProvinceLanzhou 730000China Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province Lanzhou Institute of Chemical PhysicsChinese Academy of SciencesLanzhou 730000China Comprehensive Development Office of Agriculture in Ningxia Au-tonomous Region Yinchuan 750011China

In order to extract solanesol from potato stems and leaves more effectively and improve the extraction rate of solanesol, the same batch of potato stems and leaves harvested from Guyuan, Ningxia was selected as a research object, cell wall of potato stems and leaves was broken by enzymolysis with cellulase and high- speed shearing, and then reflux-extracted with 95% ethanol. Solanesol content was determined by HPLC, and extract yield was calculated. Extract yield and solanesol extraction rate was used as an index for comparison of difference between the 2 wall-breaking methods, so as to select the optimal wall-breaking method. The results showed that enzymolysis with cellulase exhibited extraction rate and extract yield of solanesol of 91.38% and 8.02%, respectively, which were better than those under high-speed shear emulsification technique. The enzymolysis wall-breaking method has the advantages of simple operation and strong feasibility.

关键词： enzymolysis High-speed shearing Potato stems and leaves Solanesol

A Two-step Biotechnological Process for Improving Nutrition Value of Feather Meal by Bacillus licheniformis S6

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Journal of Northeast Agricultural University(English Edition) 2013年第3期20卷 71-77页

作者： Guo Gang Chu Jie Wang Jun-gao He Qiu-xia Liu Ke-chun College of Food & Bioengineering Shandong Institute of Light Industry Zhumadian Huazhong Chia Tai Co. Ltd. Biological Research Institute of Shandong Academy of Science

A two-step biotechnological process was developed using Bacillus licheniformis S6 to provide a simple and economical procedure which significantly improved feather meal nutrition value. Compared with IFM （initial feather meal） and CFM （commercial feather meal）, SFEFM （feather meal gained by solid fermentation and enzymolysis with continuous agitation） had a significant improvement （P〈0.05） in vitro digestibility, contents of oligopeptides and soluble protein released in digestive juice by pepsin- pancreatin digestion procedure, furthermore, some deficient essential amino acids in feather protein （histidine, methionine, lysine） were enhanced. Comapared with CFM, the oligopeptides released into digestive juice of ISFM （feather meal obtained by the biotechnological process described in the paper with intermittent shaking） was significantly enhanced （P〈O.05）, and its in vitro digestibility was statistically （P〉0.05） equivalent to CFM. The summary of the finding to IFM treatment and possible means of further improvements were also listed.

关键词： Bacillus licheniformis S6 enzymolysis feather meal soluble protein oligopeptide amino acid

Formation Conditions of Aspergiilus oryzae Protoplast

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Agricultural Science & Technology 2010年第3期11卷 9-10,61页

作者：屈二军李辰曦李文建冯振陈兰英河南城建学院生物工程系河南平顶山467044 中南林业科技大学生命科学与技术学院湖南长沙412006

[Objective] To explore the efficient way for the preparation of Aspergillus oryzae protoplast.[Method] The statistics of protoplast yield by the enzymolysis of mycelia with different culture time,different osmotic stabilizers and different concentrations of enzyme system was carried out in this study.[Result] The protoplast yield was higher when the mycelium was cultivated for 108 h,and the osmotic stabilizer was 0.8 mol/L of *** the enzymes system of 2 mg/ml of lysozyme,6 mg/ml of cellulose and 6 mg/ml of glusulase,the protoplast yield achieved its highest,which was up to 9.0×105 per ml.[Conclusion] The yield of protoplast was affected by the situation of mycelia themselves and external *** study had provided a basis for the preparation of a large number of active protoplasts and the further researches on cell fusion.

关键词： Aspergiilus oryzae Protoplast enzymolysis