High efficiency production of ginsenoside compound K by catalyzing ginsenoside Rb1 using snailase

作     者：Zhiguang Duan Chenhui Zhu Jingjing Shi Daidi Fan Jianjun Deng Rongzhan Fu Rong Huang Cuiying Fan 

作者机构：Shaanxi Key Laboratory of Degradable Biomedical MaterialsSchool of Chemical EngineeringNorthwest UniversityTaibai North Road 229Xi'an 710069China Shanxi R&D Center of Biomaterials and Fermentation Engineering School of Chemical Engineering Northwest UniversityTaibai North Road 229Xi'an 710069China 

出 版 物：《Chinese Journal of Chemical Engineering》 (中国化学工程学报（英文版）)

年 卷 期：2018年第26卷第7期

页      面：1591-1597页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070303[理学-有机化学] 0703[理学-化学] 

基　　金：Supported by the National Natural Science Foundation of China(21476182,21776227,21776228) Shaanxi Key Laboratory of Degradable Biomedical Materials Program(2014SZS07-K04,2014SZS07-P05,15JS106,2014SZS07-Z01,2014SZS07-Z02,2016SZSj-35,2014SZS07-K03) Shaanxi R&D Center of Biomaterials and Fermentation Engineering Program(2015HBGC-04) 

主　　题：Ginsenoside Rb1 Ginsenoside Compound K Snailase Enzymolysis 

摘      要：The rare ginsenoside Compound K （C-K） is attracting more attention because of its good physiological activity and urgent need. There are many pathways to obtain ginsenoside C-K, including chemical and biological methods. Among these, the conversion of PPD-type ginsenosides by enzymatic hydrolysis is a trend due to its high efficiency and mild conditions. For effectively extracting from the other panaxadiol saponins, the conversion process for ginsenoside C-K was investigated using snailases in this study. The univariate experimental design and response surface methodology were used to determine the optimal hydrolysis conditions for the conversion of ginsenoside Rbl into ginsenoside C-K by snailases. The optimum conditions were as follows： pH 5,12, temperature 51 ℃, ratio of snailase/substrate 0.21, and reaction time 48 h. On the basis of these parameters, the addition of 1.0 mmol· L- 1 ferric ion was found to significantly improve the enzymolysis ofsnailases for the first time. With the above conditions, the maximum conversion rate reached 89.7%, suggesting that the process can obviously increase the yield of ginsenoside C-K. The bioassay tests indicated that the ginsenoside C-K showed anti-tumor activity in a series of tumor cell lines. Based on these results, we can conclude that the process of rare ginsenoside C- K production by enzymolysis with snailase is feasible, efficient, and suitable for the industrial production and application.