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2010 the 5th International Academic Conference on Environmental and Occupational Medicine

作者： ZHEN Shi-qi,QIAN Le,YAO Mao-sheng,ZHOU Ming-hao,CHEN Lian-sheng,ZHANG Qi,ZHANG Bao-ying, CHEN Xiao-dong,YIN Li-hong 1.The School of Public Health,Shoutheast University,Nanjing 210009,Jiangsu,China 2.Jiangsu Centers for Disease Prevention and Control,Nanjing 210009,Jiangsu 3.College of Environmental Sciences and Engineering,Peking University,Beijing 100871 4.Institute for Environmental Health and Related Product Safety,China CDC,Beijing 102206,China.

＜正＞[Objective]Investigate the efficiency of a novel electrostatic sampler in detecting airborne Legionella Pneumophila.[Methods]As a reference,Biosamplers were operated in parallel with the electrostatic sampler in the key points of the central air conditioners’ *** samples collected were further amplified using nested qPCR for detecting Legionella pneumophila.[Results]For all the samples taken（414 L of air） by the electrostatic sampler,*** positive rate was 12.5%.While for the samples taken（768 L of air） by the Biosampler,the positive rate was 20.8%.[Conclusion]Compared with the Biosampler under the field sampling conditions,the new electrostatic sampler obtained relatively lower *** positive rates,which was largely due to the smaller volume of air sampled by the electrostatic sampler.

关键词： electrostatic sampler airborne legionella pneumophila nested-PCR

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Rapid monitoring of indoor airborne influenza and coronavirus with high air flowrate electrostatic sampling and PCR analysis

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Frontiers of Environmental Science & Engineering 2024年第7期18卷 73-85页

作者： Sanggwon An Sangsoo Choi Hyeong Rae Kim Jungho Hwang School of Mechanical Engineering Yonsei UniversitySeoul 02876Republic of Korea Gas Metrology Group Korea Research Institute of Standards and Science(KRISS)Daejeon 34113Republic of Korea

The World Health Organization has raised concerns about the possibility of airborne transmission in enclosed and poorly ventilated ***,rapid monitoring of airborne viruses is necessary in multi-use facilities with dense ***,an electrostatic air sampler(250 L/min)was developed in this work to obtain indoor viral aerosol samples for analysis via the Polymerase Chain Reaction(PCR).Aerosol tests with H1N1 and HCoV-229E were performed to evaluate the sample collection *** analysis,along with another aerosol test,was conducted to evaluate the recovery of the virus particles collected by the *** laboratory tests,our electrostatic sampler obtained viral samples that were detectable by PCR under the simulated viral pandemic scenario(3000 RNA copies per cubic meter of air)within 40 *** resulting cycle threshold(C)values were 35.07 and 37.1 for H1N1 and HCoV-229E,*** the performance evaluation in the laboratory,field tests were conducted in a university classroom from October 28 to December 2,*** A and HCoV-229E were detected in two air samples,and the corresponding C,values were 35.3 and *** PCR results are similar to those obtained from laboratory tests,considering the simulatedviralpandemic scenario.

关键词： Airborne virus electrostatic sampler Rapid monitoring Indoor environment Field test

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