Rapid monitoring of indoor airborne influenza and coronavirus with high air flowrate electrostatic sampling and PCR analysis
作者机构：School of Mechanical EngineeringYonsei UniversitySeoul 02876Republic of Korea Gas Metrology GroupKorea Research Institute of Standards and Science(KRISS)Daejeon 34113Republic of Korea
出 版 物：《Frontiers of Environmental Science & Engineering》 (环境科学与工程前沿（英文）)
年 卷 期：2024年第18卷第7期
页 面：73-85页
核心收录：
学科分类：0808[工学-电气工程] 0809[工学-电子科学与技术（可授工学、理学学位）] 07[理学] 08[工学] 09[农学] 0710[理学-生物学] 083002[工学-环境工程] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 0903[农学-农业资源与环境] 100402[医学-劳动卫生与环境卫生学] 0702[理学-物理学] 10[医学] 0713[理学-生态学]
基 金：This research was supported by the Korea Environment Industry and Technology Institute(KEITI)through the Technology Development Project for Biological Hazards Management in Indoor Air funded by the Korea Ministry of Environment(MOE)(No.2021003370005)
主 题：Airborne virus Electrostatic sampler Rapid monitoring Indoor environment Field test
摘 要：The World Health Organization has raised concerns about the possibility of airborne transmission in enclosed and poorly ventilated ***,rapid monitoring of airborne viruses is necessary in multi-use facilities with dense ***,an electrostatic air sampler(250 L/min)was developed in this work to obtain indoor viral aerosol samples for analysis via the Polymerase Chain Reaction(PCR).Aerosol tests with H1N1 and HCoV-229E were performed to evaluate the sample collection *** analysis,along with another aerosol test,was conducted to evaluate the recovery of the virus particles collected by the *** laboratory tests,our electrostatic sampler obtained viral samples that were detectable by PCR under the simulated viral pandemic scenario(3000 RNA copies per cubic meter of air)within 40 *** resulting cycle threshold(C)values were 35.07 and 37.1 for H1N1 and HCoV-229E,*** the performance evaluation in the laboratory,field tests were conducted in a university classroom from October 28 to December 2,*** A and HCoV-229E were detected in two air samples,and the corresponding C,values were 35.3 and *** PCR results are similar to those obtained from laboratory tests,considering the simulatedviralpandemic scenario.
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