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THE INTERRELATIONSHIP BETWEEN TUMOR CELLS'electrophoretic mobility,ADHESIVE AND INVASIVE ABILITIES,AND THEIR METASTATIC POTENTIAL

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Chinese Medical Sciences Journal 1995年第1期10卷 6-11页

作者：高进刘玉琴 Institute of Basic Medical Sciences CAMS Beijing.

Investigations on the relationship between some tumor cell properties and their metastatic potential using different clones from two tumor cell lines are reported. CNE2L2(highly metastatic) and CNE2L4 (low metastatic) clones were isolated from human nasopharyngeal carcinoma CNE-2Z cell line. LA1. LAD and LA5 clones were isolated from mouse pulmonary adenocarcinoma LA795 cell line with different metastatic potentials. The results from both cell lines showed that the metastatic potential of tumor cell positively correlated with elecirophoretic mobility rate. adhesion with endothelial cells and in vitro and in vivo invasiveness. and negatively correlated with self-adhesion and adhesion to fibroblast cells. The data further confirmed the relationship between these factors and the metastatic potential of tumor *** results provide a theoretical basis for elucidating the mechanism of metastasis.

关键词： electrophoretic mobility adhesion invasion

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Differently synthesized gold nanoparticles respond differently to functionalization with L-amino acids

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Particuology 2020年第5期18卷 97-104页

作者： Darshana V.Havaldar Reshma V.Patil Disha N.Moholkar Priyanka S.Magdum Akash P.Vadrale Kiran D.Pawar School of Nanoscience and Biotechnology Shivaji UniversityKolhapurMaharashtraIndia

The potential utility of gold nanoparticles(AuNPs)synthesized via different methods for biomedical applications vary greatly due to inherent differences in their surface *** the present study,we investigated the functionalization of AuNPs synthesized by chemical reduction,plant extract,and bacteria-mediated methods with 22 L-amino *** produced by bacteria-mediated(B_AuNPs)and plant-mediated(P_AuNPs)methods showed good potential,as they were able to be functionalized with six(histidine,lysine,methionine,phenylalanine,tryptophan,and tyrosine)and four(cystine,tryptophan,tyrosine,and valine)amino acids,*** contrast,AuNPs produced by chemical reduction(C_AuNPs)were not found to be suitable for *** functionalization conditions were found to be amino acid concentration of 20-25 mM and neutral pH(7)for P_AuNPs,whereas B_AuNPs tolerated more variable *** electrophoretic mobility of P_AuNPs after functionalization indicated that these nanoparticles were less sensitive than B_AuNPs to the deviations from optimal conditions.A significant change in mobility was observed when B_AuNPs were functionalized with either methionine or ***,the results of this study suggest that the suitability of the three differently synthesized AuNPs with amino acids is in the following order:B_AuNPs>P_AuNPs>C_AuNPs.

关键词： Gold nanoparticles Biogenic synthesis Amino acids Functionalization electrophoretic mobility

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Characterization of a Novel 1Ay Gene and Its Expression Protein in Triticum urartu

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Agricultural Sciences in China 2010年第11期9卷 1543-1552页

作者： HU Xi-gui Triticeae Research Institute Sichuan Agricultural University Wenjiang 611130 P.R. China Key Laboratory of Crop Genetic Resources and Improvement Ministry of Education/Sichuan Agricultural University Ya 'an 625014 P.R. China Key Laboratory of Adaptation and Evolution of Plateau Biota Northwest Institute of Plateau Biology Chinese Academy of Sciences Xining 810001 P.R. China

High molecular weight glutenin subunit （HMW-GS） plays an important role in determining dough property and breadmaking quality, and the exploration of novel genes for HMW-GS will be crucial for quality improvement program. A gene coding the y type HMW-GS at Glu-A1 locus in Triticum urartu （AA, 2n=2×= 14） with an electrophoretic mobility similar to that of 1Dyl2, was cloned, sequenced, and heterologously expressed. This novel active lay gene FJ404595 was confirmed by structure analyses of nucleotide and deduced amino acid sequences combining with phylogenetic analysis. The open reading frame （ORF） of this gene was 1 830 bp, encoded a protein of 608 amino acid residues containing 46 hexapeptides and 14 nonapeptides, which was mostly similar to the lAy gene AM183223 at a high identity of 99.62% with the two substitutions of both leucine/proline and valine/glutamate, obviously different from the lAy gene EU984504 with 587 residues containing 44 hexapeptides and 13 nonapeptides in T. urartu. The amino acid （leucine） at 446 differed from that （proline） of all the eight compared active lAy subunits. The predicted secondary protein structure implied that this lAy subunit might also have positive impact on flour processing quality.

关键词： Triticum urartu lAy subunit expression electrophoretic mobility secondary structure

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Surface modification of titanium dioxide for electrophoretic particles

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Frontiers of Chemical Science and Engineering 2007年第4期1卷 338-342页

作者： PENG Xuhui LE Yuan BIAN Shuguang LI Woyuan WU Wei DAI Haitao CHEN Jianfeng Key Lab for Nanomaterials Ministry of EducationBeijing University of Chemical TechnologyBeijing 100029China

To prepare stable electrophoretic ink(E Ink)needs color particles to be uniformly dispersed in the organic ***,t-he modification of inorganic particle surface is *** this paper,Titanium dioxide modified by alumina has been *** surface composition and struc-tures of modified particles have been characterized by X-ray photoelectron spectrometer(XPS),X-ray diffractometer(XRD)and Fourier transform infrared spectrometer(FT-IR).The dispersibility and electrophoretic mobility of these particles in tetrachloroethylene(TCE)have been investigated by laser particle size analyzer,static sedimentation and elec-trophoretic *** of temperature,pH value and stirring rate on the dispersibility and the charge property of samples have been *** results indicate the settle time of modified TiO2 can last 120 h with the response time of 35 s under the optimized modifying conditions,in which temperature is 85℃-90℃,pH is 8-9 and stirring rate is 800 r·min^(−1).The dispersibility and electrophoretic mobility have been significantly improved,which means that the modified TiO2 is suitable for electrophoretic ink particles.

关键词： titanium dioxide surface modification dispersibility electrophoretic mobility

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