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Hepatocyte growth factor gene therapy prevents radiation-induced liver damage

World Journal of Gastroenterology 2005年第10期11卷 1496-1502页

作者： Chau-Hua Chi I-Li Liu Wei-Yu Lo Bor-Song Liaw Yu-Shan Wang Kwan-Hwa Chi Institute of Veterinary Science Anawrahta Biotech. Co. Department of Radiation therapy and Oncology

AIM: To transfer human HGF gene into the liver of rats by direct electroporation as a means to prevent radiationinduced liver ***: Rat whole liver irradiation model was accomplished by intra-operative approach. HGF plasmid was injected into liver and transferred by electroporation using a pulse generator. Control rats (n = 8) received electrogene therapy (EGT) vehicle plasmid and another 8rats received HGF-EGT 100 μg 48 h before *** of HGF in liver was examined by RT-PCR and ELISA methods. Apoptosis was determined by TUNEL assay. Histopathology was evaluated 10 wk after whole liver ***: Marked decrease of apoptotic cells and downregulation of transforming growth factor-beta 1 (TGF-β1)mRNA were observed in the HGF-EGT group 2 d after liver irradiation compared to control animals. Less evidence of radiation-induced liver damage was observed morphologically in liver specimen 10 wk after liver irradiation and longer median survival time was observed from HGF-EGT group (14 wk) compared to control rats (5 wk). (P = 0.031).CONCLUSION: For the first time it has been demonstrated that HGF-EGT would prevent liver from radiation-induced liver damage by preventing apoptosis and down-regulation of TGF-β1.

关键词： Hepatocyte growth factor HGF Radiation Liver Electroporation electrogene therapy

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