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Hepatocyte growth factor gene therapy prevents radiation-induced liver damage

Hepatocyte growth factor gene therapy prevents radiation-induced liver damage

作     者:Chau-Hua Chi I-Li Liu Wei-Yu Lo Bor-Song Liaw Yu-Shan Wang Kwan-Hwa Chi 

作者机构:Institute of Veterinary Science Anawrahta Biotech. Co. Department of Radiation Therapy and Oncology 

出 版 物:《World Journal of Gastroenterology》 (世界胃肠病学杂志(英文版))

年 卷 期:2005年第11卷第10期

页      面:1496-1502页

核心收录:

学科分类:0831[工学-生物医学工程(可授工学、理学、医学学位)] 100207[医学-影像医学与核医学] 1006[医学-中西医结合] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 08[工学] 1010[医学-医学技术(可授医学、理学学位)] 100201[医学-内科学(含:心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 100106[医学-放射医学] 100602[医学-中西医结合临床] 10[医学] 

基  金:Supported by National Science Council grant NSC-91-275-9075-001 for the development of Boron Neutron Capture Therapy for Hepatoma Treatment 

主  题:Hepatocyte growth factor HGF Radiation Liver Electroporation Electrogene therapy 

摘      要:AIM: To transfer human HGF gene into the liver of rats by direct electroporation as a means to prevent radiationinduced liver ***: Rat whole liver irradiation model was accomplished by intra-operative approach. HGF plasmid was injected into liver and transferred by electroporation using a pulse generator. Control rats (n = 8) received electrogene therapy (EGT) vehicle plasmid and another 8rats received HGF-EGT 100 μg 48 h before *** of HGF in liver was examined by RT-PCR and ELISA methods. Apoptosis was determined by TUNEL assay. Histopathology was evaluated 10 wk after whole liver ***: Marked decrease of apoptotic cells and downregulation of transforming growth factor-beta 1 (TGF-β1)mRNA were observed in the HGF-EGT group 2 d after liver irradiation compared to control animals. Less evidence of radiation-induced liver damage was observed morphologically in liver specimen 10 wk after liver irradiation and longer median survival time was observed from HGF-EGT group (14 wk) compared to control rats (5 wk). (P = 0.031).CONCLUSION: For the first time it has been demonstrated that HGF-EGT would prevent liver from radiation-induced liver damage by preventing apoptosis and down-regulation of TGF-β1.

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