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Bioinformatic analysis and functional characterization of CFEM proteins in Setosphaeria turcica

Journal of Integrative Agriculture 2021年第9期20卷 2438-2449页

作者： WANG Jian-xia LONG Feng ZHU Hang ZHANG Yan WU Jian-ying SHEN Shen DONG Jin-gao HAO Zhi-min State Key Laboratory of North China Crop Improvement and Regulation/Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology College of Life SciencesHebei Agricultural UniversityBaoding 071001P.R.China College of Plant Protection Hebei Agricultural UniversityBaoding 071001P.R.China

Common in Fungal Extracellular Membrane(CFEM)domains are uniquely found in fungal extracellular membrane proteins which are important for *** study identified 13 StCFEM proteins in the genome of Setosphaeria turcica,the hemibiotrophic fungus that causes northern corn leaf *** alignment and WebLogo analysis of their CFEM domains indicated that the amino acids were highly conserved and that,with the exception of StCFEM1,2,3,and 6,they contained eight *** analysis suggested that these 13 proteins(StCFEM1–13)could be divided into 2 clades based on the presence of the trans-membrane *** StCFEM proteins with a signal peptide and without a trans-membrane domain were considered as candidate effector *** CFEM domain in the candidate effector proteins could form a helical-basket structure homologous to Csa2 in Candida *** analysis suggested that the 13 genes were expressed during fungal infection and a yeast secretion assay revealed that these candidate effectors were secreted *** was also found that StCFEM3,4,and 5 couldn't affect BAX/INF1-induced programmed cell death(PCD)in Nicotiana benthamiana and while StCFEM12 could suppress INF1-induced PCD,it showed no effect on BAXinduced *** study found that there were 13 members of the *** CFEM protein family and that StCFEM12 was a candidate *** study laid the foundation for illustrating the roles of CFEM proteins during the pathogenic processes of phytopathogens.

关键词： CFEM domain effector Setosphaeria turcica signal peptide PCD

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Genetic variation in GmCRP contributes to nodulation in soybean(Glycine max Merr.)

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The Crop Journal 2023年第2期11卷 332-344页

作者： Jinhui Wang Chao Ma Shengnan Ma Haiyang Zheng Huilin Tian Xi Wang Yue Wang Hongwei Jiang Jiangxu Wang Zhanguo Zhang Chunyan Liu Mingliang Yang Qingshan Chen Dawei Xin Key Laboratory of Soybean Biology of Chinese Ministry of Education Key Laboratory of Soybean Biology and Breeding/Genetics of Chinese Agriculture MinistryCollege of AgricultureNortheast Agricultural UniversityHarbin 150030HeilongjiangChina Jilin Academy of Agricultural Sciences Changchun 130033JilinChina Heilongjiang Academy of Agricultural Sciences Harbin 150086HeilongjiangChina

Symbiosis between soybean and rhizobia contributes to soybean yield and quality. Although secreted rhizobial type Ⅲ effectors are known to regulate infection and promote nitrogen fixation, much about them remains unknown. Mutation of NopC, a type Ⅲ effector from Sinorhizobium fredii HH103, reduced nodule numbers and dry weights in 310 soybean accessions, and expression of NopC in soybean hairy roots promoted symbiosis. Based on observed differences in nodule traits between Suinong 14 and Zyd 00,006inoculated with HH103 and the NopC mutant, 11 QTL associated with rhizobia were identified in chromosome segment substitution lines(CSSLs) derived from Suinong 14 and Zyd 00006. Using chromosome fragment insertion, whole-genome sequencing of Suinong 14 and Zyd 00006, and qRT-PCR,Glyma.19G176300(GmCRP) was identified as a candidate gene associated with NopC, and GmCRP was found to be induced by NopC to positively regulate nodulation. SNPs located in the regulatory regions of GmCRP influenced its expression response to NopC, with SNPs contributing to nodulation having been selected during domestication. Our findings reveal the function of a soybean gene encoding a rhizobial type Ⅲ effector that contributes to symbiosis, and will facilitate the practical application of symbiotic nitrogen fixation in molecular breeding.

关键词： Soybean Symbiosis effector NopC Natural varieties

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Lineage relationship of CD8^＋ T cell subsets is revealed by progressive changes in the epigenetic landscape

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Cellular & Molecular Immunology 2016年第4期13卷 502-513页

作者： Joseph G. Crompton Manikandan Narayanan Suresh Cuddapah Rahul Roychoudhuri Yun Ji Wenjing Yang Shashank J. Patel Madhusudhanan Sukumar Douglas C. Palmer Weiqun Peng Ena Wang Francesco M. Marincola Christopher A. Klebanoff Keji Zhao John S. Tsang Luca Gatt-inoni Nicholas P. Restifo Surgery Branch National Cancer Institute National Institutes of Health Bethesda MD 20892 USA Cambridge Institute for Medical Research University of Cam bridge Cambridge CB20XY UK Systems Genomics and Bioinformatics Unit Laboratory of Systems Biology National Institute of Allergy and Infectious Diseases National Institutes of Health Bethesda MD 20892 USA Department of Environmental Medicine New York University School of Medicine Tuxedo NY 10987 USA Experimental Transplantation and Immunology Branch National Cancer Institute National Institutes of Health Bethesda MD 20892 USA Department of Physics The George Washington University Washington DC 20052 USA Research Branch Sidra Medical and Research Centre Doha Qatar Infectious Disease and Immunogenetics Section Department of Transfusion Medicine Clinical Center National Institutes of Health Bethesda MD 20892 USA Clinical Investigator Development Program NCI/NIH Bethesda MD 20892 USA Systems Biology Center National Heart Lung and Blood Institute National Institutes of Health Bethesda MD 20892 USA. Lombardi Comprehensive Cancer Center Georgetown University Washington. DC 20007. United States. Bethesda. MD 20892. USA.

To better elucidate epigenetic mechanisms that correlate with the dynamic gene expression program observed upon T-cell differentiation, we investigated the genomic landscape of histone modifications in naive and memory CD8＋ T cells. Using a ChlP-Seq approach coupled with global gene expression profiling, we generated genome-wide histone H3 lysine 4 （H3K4me3） and H3 lysine 27 （H3K27me3） trimethylation maps in naive, T memory stem cells, central memory cells, and effector memory cells in order to gain insight into how histone architecture is remodeled during T cell differentiation. We show that H3K4me3 histone modifications are associated with activation of genes, while H3K27me3 is negatively correlated with gene expression at canonical loci and enhancers associated with T-cell metabolism, effector function, and memory. Our results also reveal histone modifications and gene expression signatures that distinguish the recently identified T memory stem cells from other CD8＋ T-cell subsets. Taken together, our results suggest that CD8＋ lymphocytes undergo chromatin remodeling in a progressive fashion. These findings have major implications for our understanding of peripheral T-cell ontogeny and the formation of immunological memory.

关键词： TRANSCRIPTION FACTOR STEM-CELLS MEMORY effector DIFFERENTIATION BET FATE LYMPHOCYTES MAINTENANCE GENERATION

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Baicalin extracted from Huangqin(Radix Scutellariae Baicalensis) induces apoptosis in gastric cancer cells by regulating B cell lymphoma(Bcl-2)/Bcl-2-associated X protein and activating caspase-3 and caspase-9

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Journal of Traditional Chinese Medicine 2017年第2期37卷 229-235页

作者： Wang Hongwei Li Hailong Chen Fengqin Luo Jun Gu Jing Wang Huping Wu Hongyan Xu Yan Clinical Laboratory The Hospital Affiliatedto Gansu University of Chinese Medicine Lanzhou 730000China Key Laboratory for Transfer of Dunhuang Medicine atthe Provincial and Ministerial Level Lanzhou 730000 China Department of Teaching and Research of Clinical Laboratory School of Clinical Medicine Gansu Universityof Chinese Medicine Lanzhou 730000 China Clinical Laboratory The Hospital Affiliated to Gansu University of ChineseMedicine Lanzhou 730000 China Key Laboratory for Transfer of Dunhuang Medicine at the Provincial and MinisterialLevel Lanzhou 730000 China Key Laboratoryfor Transfer of Dunhuang Medicine at the Provincia andMinisterial Level Lanzhou 730000 China Gansu Provincial Key Labora-tory of Chinese Medicinal Herbs for Prescription Innovationand Transformation Gansu University of Chinese MedicineLanzhou 730000 China

OBJECTIVE:To evaluate the effects of baicalin in human gastric cancer cells, including apoptosis-inducing effects, and to investigate its underlying mechanisms of ***:Cell proliferation and apoptosis assays were performed to investigate the anti-proliferation effects of baicalin in human gastric cancer BGC-823 and MGC-803 *** time-quantitative polymerase chain reaction and Western blotting analysis were performed to elucidate the molecular mechanisms underlying the anti-tumor properties of ***:In BGC-823 and MGC-803 gastric cancer cells treated with 80, 120, and 160 μmol/L baicalin for 48 h, a 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide assay showed that baicalin significantly inhibited cell proliferation in a dose-dependent manner, while flow cytometric analysis demonstrated that baicalin could induce apoptosis, also in a dose-dependent ***, baicalin up-regulated the expression of caspase-3, caspase-9, and B cell lymphoma(Bcl-2)-associated X protein and down-regulated the expression of Bcl-2 at both the m RNA and protein ***:Baicalin has potential as a therapeutic agent for gastric cancer by inducing apoptosis in cancer cells.

关键词： Baicalin Stomach neoplasms Apoptosis Lymphoma B-Cell Bcl-2-associated X protein Caspases effector

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Bacterial Vector-Borne Plant Diseases:Unanswered Questions and Future Directions

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Molecular Plant 2020年第10期13卷 1379-1393页

作者： Weijie Huang Paola Reyes-Caldas Marina Mann Shirin Seifbarghi Alexandra Kahn Rodrigo P.P.Almeida Laure Béven Michelle Heck Saskia A.Hogenhout Gitta Coaker Department of Crop Genetics John Innes CentreNorwich Research ParkNorwich NR47UHUK Department of Plant Pathology University of CaliforniaDavisCA95616USA Department of Plant Pathology and Plant-Microbe Biology Cornell UniversityIthacaNY 14853USA Department of Environmental Science Policy and ManagementUniversity of CaliforniaBerkeleyCA 94720USA UMR 1332 Biologie du Fruit et Pathologie Univ.BordeauxINRAEVillenave d'Ornon 33882 France Boyce Thompson Institute IthacaNY 14853USA Emerging Pests and Pathogens Research Unit Robert W.Holley CenterUSDA ARSIthacaNY 14853USA School of Environmental Sciences University of East AngliaNorwich Research ParkNorwich NR47T JUK

Vector-borne plant diseases have significant ecological and economic impacts,affecting farm profitability and forest composition throughout the *** vector-borne pathogens have evolved sophisticated strategies to interact with their hemipteran insect vectors and plant *** pathogens reside in plant vascular tissue,and their study represents an excellent opportunity to uncover novel biological mechanisms regulating intracellular pathogenesis and to contribute to the control of some of the world's most invasive emerging *** this perspective,we highlight recent advances and major unanswered questions in the realm of bacterial vector-borne disease,focusing on liberibacters,phytoplasmas,spiroplasmas,and Xylella fastidiosa.

关键词： liberibacter phytoplasma spiroplasma Xylella fastidiosa vector-borne disease effector plant immunity

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Signaling in Plant Disease Resistance and Symbiosis

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Journal of Integrative Plant Biology 2008年第7期50卷 799-807页

作者： SongziZhao Xiaoquan Qi Key Laboratory of Photosynthesis and Environmental Molecular Physiology Instfute of Botany the Chinese Academy of Sciences Beijing 100093 China

Interactions between plants and microbes result in plant disease and symbiosis. The former causes considerable economic damage in modern agriculture, while the latter has produced great beneficial effects to our agriculture system. Comparison of the two interactions has revealed that a common panel of signaling pathways might participate in the establishment of the equilibrium between plant and microbes or its break-up. Plants appear to detect both pathogenic and symbiotic microbes by a similar set of genes. All symbiotic microbes seem to produce effectors to overcome plant basal defenses and it is speculated that symbiotic effectors have functions similar to pathogenic ones. Signaling molecules, salicylic acid （SA）, jasmonic acid （JA） and ethylene （ET）, are involved in both plant defense and symbiosis. Switching off signals contributing to deterioration of disease symptom would establish a new equilibrium between plant and pathogenic microbes. This would facilitate the development of strategies for durable disease resistance.

关键词： calcium signals disease resistance effector pathogen-associated molecular patterns symbiosis.

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The key residues of OsTFIIAγ5/Xa5 protein captured by the argininerich TFB domain of TALEs compromising rice susceptibility and bacterial pathogenicity

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Journal of Integrative Agriculture 2019年第6期18卷 1178-1188页

作者： TIAN Jing-jing HUI Shu-gang SHI Ya-rui YUAN Meng National Key Laboratory of Crop Genetic Improvement National Center of Plant Gene Research (Wuhan)Huazhong Agricultural University

Xanthomonas bacteria secrete transcription activator-like effector(TALE)proteins into host cells to activate plant disease susceptibility genes to cause disease,and the process is dependent on interaction between bacteria TFB domain of TALEs and host plant basal transcription factor IIA gamma subunit(TFIIAγ).The key domain or residues of plant TFIIAγand core residues of bacteria TFB domain that are indispensable for TFIIAγ-TALEs interaction in the process of TALE-carrying Xanthomonas invasion plants are ***,we showed that the thirdα-helix domain of OsTFIIAγ5/Xa5,especially the 38th,39th,40th and 42th residues were key sites for capturing by TALEs of Xanthomonas oryzae ***(Xoo),the causal agent of rice bacterial blight *** latter segment of Xoo TFB domain harboring seventy-two amino acid residues was vital for TALE specific binding with host plant OsTFIIAγ5/*** of some residues in this core region of TFB domain completely compromised capacity of TALEs capturing rice OsTFIIAγ5/*** rich and conserved arginine residues in this core region of TFB domain were responsible for TALE-dependent plant susceptibility gene activation and virulence of *** results provide a potential strategy for improving resistance to TALE-carrying pathogens in plants by site-specific modification of key residues of host plant TFIIAγ.

关键词： Xanthomonas transcription activator-like effector PthXo1 TFB rice

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Construction and characterization of a hrpG mutant rendering constitutive expression of hrp genes in Xanthomonas campestris ***

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Progress in Natural Science:Materials International 2006年第5期16卷 480-485页

作者： JIANG Bole, XU Rongqi, LI Xianzhen, WEI Hongyu, BAI Faan, HU Xi, HE Yongqiang and TANG Jiliang ( The Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization,College of Life Science and Technology of Guangxi University, Nanning 530004, China) The Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization Nanning 530004 China College of Life Science and Technology of Guangxi University Nanning 530004 China

The expression of hrp genes in Xanthomonas is regulated by hrpG, an OmpR-type transcriptional activator, and can be induced by artificial mimic medium XVM2, but repressed in rich medium NYG. In this study, a site-specific mutation of hrpG in Xanthomonas campestris pv. campestris (Xcc) was generated by PCR-directed point mutagenesis and was characterized by its phenotype and transcriptional expression analyses. The results indicated that the E45K mutated hrpG rendered all the hrp genes expressing constitutively in the suppression medium NYG. Plant test showed that mutated hrpG enhanced the timing and intensity of hypersensitive reaction (HR) on the nonhost plant pepper, but did not affect the growth of Xcc in both culture medium and plants. The E45K mutation did not cause significant changes of production of exopolysaccharide, extracellular cellulase and amylase, but it inhibited the secretion of extracellular protease. This mutant 8004 is more suitable than the wild type for identifying hrp-dependent effectors in vitro and studying the transcriptional regulation genome-wide.

关键词： hrpG Xanthomonas campestris pv. campestris effector constitutive expression site-specific mutagenesis.

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Fg12 ribonuclease secretion contributes to Fusarium graminearum virulence and induces plant cell death

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Journal of Integrative Plant Biology 2021年第2期63卷 365-377页

作者： Bo Yang Yuyin Wang Mengjun Tian Kaixin Dai Wenyue Zheng Zehan Liu Sen Yang Xinyu Liu Dongya Shi Haifeng Zhang Yan Wang Wenwu Ye Yuanchao Wang Department of Plant Pathology Nanjing Agricultural UniversityNanjing 210095China The Key Laboratory of Plant Immunity Nanjing Agricultural UniversityNanjing 210095China

Filamentous fungal pathogens secrete effectors that modulate host immunity and facilitate infection. Fusarium graminearum is an important plant pathogen responsible for various devastating diseases. However, little is known about the function of effector proteins secreted by F. graminearum. Herein, we identified several effector candidates in the F. graminearum secretome. Among them, the secreted ribonuclease Fg12 was highly upregulated during the early stages of F. graminearum infection in soybean;its deletion compromised the virulence of F. graminearum. Transient expression of Fg12 in Nicotiana benthamiana induced cell death in a light-dependent manner. Fg12 possessed ribonuclease(RNase) activity, degrading total RNA. The enzymatic activity of Fg12 was required for its cell death-promoting effects. Importantly, the ability of Fg12 to induce cell death was independent of BAK1/SOBIR1, and treatment of soybean with recombinant Fg12 protein induced resistance to various pathogens, including F. graminearum and Phytophthora sojae. Overall, our results provide evidence that RNase effectors not only contribute to pathogen virulence but also induce plant cell death.

关键词： cell death effector fungal ribonuclease Fusarium graminearum virulence

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Structure-function Aspects of Extracellular Leucine-rich Repeat-containing Cell Surface Receptors in Plants

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Journal of Integrative Plant Biology 2013年第12期55卷 1212-1223页

作者： Zhao Zhang Bart P.H.J.Thomma Laboratory of Phytopathology Wageningen University Centre for BioSystems Genomics

Plants exploit several types of cell surface receptors for perception of extracellular signals, of which the extracellular leucine-rich repeat （eLRR）-containing receptors form the major class. Although the function of most plant eLRR receptors remains unclear, an increasing number of these receptors are shown to play roles in innate immunity and a wide variety of developmental processes. Recent efforts using domain swaps, gene shuffling analyses, site-directed mutagenesis, interaction studies, and crystallographic analyses resulted in the current knowledge on ligand binding and the mechanism of activation of plant eLRR receptors. This review provides an overview of eLRR receptor research, specificallysummarizing the recent understanding of interactions among plant eLRR receptors, their co-receptors and corresponding ligands. The functions of distinct eLRR receptor domains, and their role in structure, ligand perception and multimeric complex formation are discussed.

关键词： eLRR receptor-like kinase (RLK) receptor-like protein (RLP) pathogen-associated molecular pattern (PAMP) effector peptide ligand brassinosteroid CLE peptide.

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