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中国植物病理学会2019年学术年会

作者： Zhang Jin Yang Piao Wu Huiming Fang Wenqin Zheng Wenhui State Key Laboratory of Ecological pest Control for Fujian and Taiwan Crops College of Plant ProtectionFujian Agriculture and Forestry University

SNAREs（soluble N-ethylmaleimide-sensitive factor attachment protein receptors） belong to a superfamily of transmembrane proteins locating on organelles and vesicular *** anchoring and fusion mediated by SNAREs are the main ways how materials and information are exchanged between organelles in endocytic secretory pathways of eukaryotic *** cerevisiae involves two v-SNAREs（Sncl,Snc2）,but only one v-SNARE exists in the rice blast fungus Magnaporthe oryzae（named MoSnc1） and has not been systematically characterized so *** this study,we generated MoSNC1 gene knockout mutant in the *** analyses revealed that the fungal growth,conidiation and pathogenicity of AMosncl all decreased to some extents compared with that of the wild type Guy ***,we observed that MoSncl-GFP localized in a bright fluorescent punctum close to the tips of growing and invasive *** expressed the plasmid vector containing both of cytoplasmic effector Pw12-mCherry and apoplastic effector Bas4-GFP in the AMosncl and checked their localizations using a high-resolution laser confocal *** found that in AMosncl,Bas4-GFP was not completely located within the extra-invasive hyphal membrane（EIHM）,but partially mistakenly located into the vacuoles,and Pw12-mCherry showed several biotrophic interfacial complex（BICs）-like localizations,suggesting that MoSnc 1 is required for correct localizations of cytoplasmic and apoplastic effectors in *** the future,the mechanism of how SNARE proteins function in secretion of effector proteins of *** will be further studied,so as to pursue a clearer understanding of effector’s translocation in the rice blast fungus.

关键词： Magnaporthe oryzae MoSnc1 SNARE effector

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Molecular Cloning and Bioinformatics Analysis of TypeⅢSecretion System effector Protein HY 322 Gene of Vibrio alginolyticus

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Agricultural Biotechnology 2018年第6期7卷 1-5,8页

作者： Shanshan LIANG Mingjie FAN Weijian LIANG Huanying PANG Chuanhao PAN Dawei SONG Mingsheng QIU Jichang JIAN Shenzhen Institute of Guangdong Ocean University Shenzhen 510000China Fisheries College of Guangdong Ocean University Zhanjiang524088China Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemilogy for Aquatic Economic Animals/Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes Zhanjiang 524088China

In this study,Hy322 gene was cloned from Vibrio *** total length of its gene was 969 bp,and it could encode 322 amino *** physicochemical properties,protein structure,genetic evolutionary relationship and antigenic characteristics of the effector protein Hy322 of *** HY9901 type Ⅲ secretion system were studied and analyzed by bioinformatics methods and *** results showed that Hy322 is an unstable hydrophilic and acidic protein without a transmembrane region and a signal peptide,and secondary structure to α-*** evolutionary analysis showed that *** HY9901 and *** were clustered together,which indicated that the genetic relationship between the two species was ***322 contains a FliN super family conserved domain associated with Flagellar motor *** analysis showed that the B-cell preponderant epitopes of Hy322 might be localized in the regions of 32-33,100-102,138-140,215-216,235-238 and *** 3D structure model of Hy322 subunit was simulated by SWISS-MODEL software and itwas found that the yscQ of Yersinia were similar and the similarity was 42.25%.In this study,the feasibility of Hy322 as a common antigen of Vibrio was verified from the perspective of bioinformatics,which laid the foundation for the next step in vaccine development.

关键词： Vibrio alginolyticus TypeⅢsecretory system effector protein Bioinformatics analysis

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A Nucleus-localized effector from Phytophthora sojae Recruits a N-acetyltransferase Into Nucleus to Suppress Plant Immunity

A Nucleus-localized Effector from Phytophthora sojae Recruit...

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中国植物病理学会2016年学术年会

作者： LI Hai-yang WANG Hao-nan JING Mao-feng KONG Liang GUO Bao-dian WANG Yang LIN Ya-chun CHEN Han YANG Bo KONG Guang-hui ZHAO Yao MA Zhen-chuan WANGYan YE Wen-wu DONG Suo-meng WANG Yuan-chao Department of Plant Pathology Nanjing Agricultural University

Filamentous fungi and oomycete pathogens secrete many intracellular effectors to manipulate host immunity during *** of plant targets of these effectors will help to uncover the mechanisms on how effectors suppress PAMP-triggered immunity（PTI） and effector-triggered immunity（ETI） in *** we have determined that PsAvh52,an effector secreted from soybean root rot pathogen Phytophthora sojae,can suppress the cell death induced by both the effectors（Avh238 or Avh241） and PAMPs（INF1 and XEG1） in Nicotiana ***,we demonstrated that PsAvh52 interacts with a soybean histone acetyltransferase（GmHAT1）,a key factor manipulating epigenetic ***1 localizes in plant cell cytoplasm,but it was translocalized from the cytoplasm to nucleus when co-expressed with *** nucleus-localized GmHAT1 regulates the expression of plant defense-related genes and attenuates plant resistance by acetylating *** together,these results indicate that PsAvh52 manipulates epigenetic modifications to enhance Phytophthora sojae colonization in soybean.

关键词： Phytophthora sojae effector plant defense epigenetic modifications

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NLR immune receptor RB is differentially targeted by two homologous but functionally distinct effector proteins

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Plant Communications 2021年第6期2卷 56-70页

作者： Jinping Zhao Junqi Song Texas A&M AgriLife Research Center at Dallas DallasTX 75252USA Department of Plant Pathology and Microbiology Texas A&M UniversityCollege StationTX 77843USA

Plant nucleotide-binding leucine-rich repeat(NLR)receptors mediate immune responses by directly or indirectly sensing pathogen-derived *** significant advances in the understanding of NLR-mediated immunity,the mechanisms by which pathogens evolve to suppress NLR activation triggered by cognate effectors and gain virulence remain largely *** agronomically important immune receptor RB recognizes the ubiquitous and highly conserved IPI-O RXLR family members(e.g.,IPI-O1)from Phytophthora infestans,and this process is suppressed by the rarely present and homologous effector IPI***,we report that self-association of RB via the coiled-coil(CC)domain is required for RB activation and is differentially affected by avirulence and virulence ***-O1 moderately reduces the self-association of RB CC,potentially leading to changes in the conformation and equilibrium of RB,whereas IPIO4 dramatically impairs CC self-association to prevent RB *** also found that IPI-O1 associates with itself,whereas IPI-O4 does ***,IPI-O4 interacts with IPI-O1 and disrupts its self-association,therefore probably blocking its avirulence ***,IPI-O4 enhances the interaction between RB CC and IPI-O1,possibly sequestering RB and IPI-O1 and subsequently blocking their interactions with signaling *** together,these findings considerably extend our understanding of the underlying mechanisms by which emerging virulent pathogens suppress the NLR-mediated recognition of cognate effectors.

关键词： RB nucleotide-binding leucine-rich repeat receptor self-association coiled-coil domain Phytophthora infestans effector

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Gene clone and eukaryotic expression of a putative effector g10687 from Cylindrocladium parasiticum

Gene clone and eukaryotic expression of a putative effector ...

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多彩菌物美丽中国——中国菌物学会2019年学术年会

作者：陈欣瑜向梅梅董章勇 Institute of Plant Health Zhongkai University of Agriculture and Engineering

The objective of this study is to clone and construct eukaryotic expression plasmids of a putative effector g10687 from Cylindrocladium parasiticum（isolate: Cylpar14017）. The full length DNA and c DNA of g10687 were cloned by PCR. Bioinformatics analysis revealed that the gene contained 812 bp, of which the ORF was 753 bp encoding 251 amino acids with hypothetical molecular weight of 26 *** deduced amino acids sequence of the protein had a transmembrane region and signal peptide. The complete ORF of g10687 was amplified by RT-PCR, and ligated to the eukaryotic expression vector pGAPZαA. The recombinant plasmid was identified successfully by restriction enzyme digestion and sequencing. Subsequently, the recombinant plasmid was transferred into Pichia pastoris strains of X33 by Gene Pulser Xcell. According to SDS-PAGE analysis, target protein of the recombinant g10687 protein could be observed after 24 h hours induced by Glycerol. Based on the result, it should be possible and potential to express this effector of g10687 by Pichia pastoris.

关键词： Cylindrocladium parasiticum effector eukaryotic expression

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Identification and Characterization of Plant Cell Death-Inducing effector proteins from Rhizoctonia solani

Identification and Characterization of Plant Cell Death-Indu...

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中国植物病理学会2017年学术年会

作者： Li Shuai Fang Rui Jia Shentong Wei Songhong College of Plant Protection Shenyang Agricultural University

Rice sheath blight,caused by Rhizoctonia solani,is one of the most important fungus diseases of ***,little has been known about molecular mechanisms underlying the function（s） of effectors in *** virulence pathogenicity so *** and characterization of novel effector proteins are crucial for understanding pathogen virulence and host-plant defense *** previous studies,genome of *** AG1 IA reveals many potential genes coding *** analysis indicated that some effectors contain structure which may contribute to ***,we performed functional studies on putative effectors in *** and demonstrated that 3 of 40 putative effectors caused necrosis or necrosis-like phenotypes in Nicotiana *** them,1 protein signal peptide play important role in triggering cell death in *** results will provide useful information for insight into pathogenic factors for ***.

关键词： Rhizoctonia solani effector Plant Immunity Pathogenicity

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Genome-wide identification of the putative effector genes in Nimbya alternantherae

Genome-wide identification of the putative effector genes in...

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中国菌物学会第七届全国会员代表大会暨2017年学术年会

作者： Zhangyong Dong Yongxin Xiao Yunxia Zhang Meimei Xiang Department of Plant Protection Zhongkai University of Agriculture and Engineering

Nimbya alternantherae is a potential biological control fungus for alligator weed（Alternanthera philoxeroides） and produces a wide range of genes encoding effector proteins capable for pathogenicity. In this study, we decided to perform a genome analysis on N. alternantherae（NA2） in order to identify putative effector genes. A total of 10,470 high confidence protein-coding genes from 34 mega base draft genome assembly of NA2 were predicted which includes a large and diverse inventory of secretory proteins, transporters and primary and secondary metabolism enzymes. We broadly define effectors as any fungal protein that is secreted by the fungal cell to act on host-derived substrates or targets or otherwise affect host responses. First, effector predictions were performed by identifying proteins with a predicted signal peptide, no transmembrane domain and no mitochondria targeting motif. Second, effector proteins showed high cysteine content（at least 6 cysteines）. Third, effectors were small proteins that less than 300 amino acid. Finally, comprehensive analysis predicted a total of 110 putative effector proteins. Our analyses would broadly expand our knowledge and will provide useful information for managing alligator weed, and insights into the pathogenesis of fungal phytopathogens for biological control.

关键词： Nimbya alternantherae effector genome

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TOPLESS promotes plant immunity by repressing auxin signaling and is targeted by the fungal effector Naked1

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Plant Communications 2022年第2期3卷 92-111页

作者： Fernando Navarrete Michelle Gallei Aleksandra EKornienko Indira Saado Mamoona Khan Khong-Sam Chia Martin A.Darino Janos Bindics Armin Djamei Gregor Mendel Institute(GMI) Austrian Academy of Sciences(OEAW)Vienna BioCenter(VBC)Dr.Bohr-Gasse 31030 ViennaAustria Leibniz Institute of Plant Genetics and Crop Plant Research(IPK) OT GaterslebenCorrensstraße 306466 Stadt SeelandGermany Present address:Institute of Science and Technology Austria Am Campus 13400 KlosterneuburgAustria Present address:Institute of Molecular Biotechnology Dr.Bohr-Gasse 31030 ViennaAustria Present address:Institute of Crop Science and Resource Conservation University of BonnBonnGermany

In plants,the antagonism between growth and defense is hardwired by hormonal *** perception of pathogen-associatedmolecularpatterns(PAMPs)frominvadingmicroorganismsinhibits auxin signalingand plant ***,pathogens manipulate auxin signaling to promote disease,but how this hormone inhibits immunity is not fully *** maydis is a maize pathogen that induces auxin signaling in its *** characterized a *** effector protein,Naked1(Nkd1),that is translocated into the host *** its native ethylene-responsive element binding factor-associated amphiphilic repression(EAR)motif,Nkd1 binds to the transcriptional co-repressors TOPLESS/TOPLESS-related(TPL/TPRs)and prevents the recruitment of a transcriptional repressor involved in hormonal signaling,leading to the derepression of auxin and jasmonate signaling and thereby promoting susceptibility to(hemi)biotrophic pathogens.A moderate upregulation of auxin signaling inhibits the PAMP-triggered reactive oxygen species(ROS)burst,an early defense ***,our findings establish a clear mechanism for auxin-induced pathogen *** Nkd1 variants with increased expression or increased EAR-mediated TPL/TPR binding trigger typical salicylic-acid-mediated defense reactions,leading to pathogen *** implies that moderate binding of Nkd1 to TPL is a result of a balancing evolutionary selection process to enable TPL manipulation while avoiding host recognition.

关键词： topless auxin pattern-triggered immunity PTI effector maize Ustilago maydis

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Genome-wide identification and expression profile of the putative effector genes in Cylindrocladium parasiticum

Genome-wide identification and expression profile of the put...

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中国菌物学会2018年学术年会

作者：陈欣瑜向梅梅董章勇 Department of Plant Protection Zhongkai University of Agriculture and Engineering

Cylindrocladium parasiticum is an important pathogen of peanut（Arachis hypogaea） causing the disease Cylindrocladium black rot and produces a wide range of genes encoding effector proteins capable for pathogenicity. In this study, we decided to perform a genome analysis on C. parasiticum（isolate: Cylpar14017） in order to identify putative effector genes. Furthermore, we analyzed RNAseq data derived from Cylpar14017-infected susceptible and resistant peanut cultivars. A total of 18,366 high confidence protein-coding genes from 63 mega base draft genome assembly of Cylpar14017 were predicted which includes a large and diverse inventory of secretory proteins, transporters, Carbohydrate-active enzymes proteins and primary and secondary metabolism enzymes. We broadly define effectors as any fungal protein that is secreted by the fungal cell to act on host-derived substrates or targets or otherwise affect host responses. In total, 1718 proteins of the18,366 proteins from Cylpar14017 were predicted to be secreted with a predicted signal peptide, no transmembrane domain and no mitochondria targeting motif. Subsequently, 137 putative effector candidates were selected on the basis of being high cysteine content（at least 6 cysteines） and high repeats（at least 9）. The relative expression for all 137 effector genes was clustered using unsupervised hierarchical clustering and presented as a heatmap. Unsupervised hierarchical clustering was performed on the data presented as FPKM of effector genes and FPKM of Actin. To validate the expression profile obtained by RNAseq analysis, qRT-PCR was performed using 11 genes chosen among putative effector genes. Expression profiles were almost agreement with the RNAseq data for these 11 genes evaluated.

关键词： Cylindrocladium parasiticum effector genome RNAseq

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A novel effector CsSp1 from Bipolaris sorokiniana was essential for colonization in wheat and also an elicitor activating host immunity through salicylic signaling pathway

A novel effector CsSp1 from Bipolaris sorokiniana was essent...

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中国植物病理学会2021年学术年会

作者： ZHANG Wanying LI Haiyang WANG Limin XIE Shunpei ZHANG Yuan KANG Ruijiao ZHANG Mengjuan ZHANG Panpan LI Yonghui HU Yanfeng WANG Min CHEN Linlin YUAN Hongxia DING Shengli LI Honglian Henan Agricultural University/Collaborative Innovation Center of Henan Grain Crops/National Key Laboratory of Wheat and Maize Crop Science Xuchang Vocational Technical College

The hemibiotrophic pathogen Bipolaris sorokiniana cause root rot,leaf blotch,and black embryo on wheat and barley globally,resulting in significant yield or quality *** mechanism of the host-pathogen interaction between *** and wheat or barley remains *** *** genome encodes a large number of uncharacterized putative effector *** this study,we found a putative secreted protein CsSpl with a classical N termina signal peptide,which is induced during early *** split-marker approach was used to knock out the CsSPl in the Lankao9-3 *** deletion mutant ΔCsspl displayed lower radial growth rate on PDA plate and produced less spores,and the complemented transformants totally restored the phenotypes of deletion mutant to that of the wild *** pathogenicity of the deletion mutant on wheat was attenuated even though appressoria penetrated the *** well,the infectious hyphae became swollen and exhibited reduced growth within the onion epidermal *** peptide of CsSpl were verified through the yeast YTK12 secretion *** expression of CsSpl in Nicotiana benthamiana inhibited lesion formation caused by Phytophthora capsica,which located in plant *** *** infected wheat leaves,the salicylic acid regulated genes,TaPAL,TaPR1 and TaPR2,were down regulated in ΔCssp1 In conclusion,the CsSpl was a virulence effector and acted as a novel elic tor to trigger host immunity through SA signaling pathway.

关键词： Bipolaris sorokiniana effector elicitor wheat root rot CsSp1 plant immune SA

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