TOPLESS promotes plant immunity by repressing auxin signaling and is targeted by the fungal effector Naked1

作     者：Fernando Navarrete Michelle Gallei Aleksandra EKornienko Indira Saado Mamoona Khan Khong-Sam Chia Martin A.Darino Janos Bindics Armin Djamei 

作者机构：Gregor Mendel Institute(GMI)Austrian Academy of Sciences(OEAW)Vienna BioCenter(VBC)Dr.Bohr-Gasse 31030 ViennaAustria Leibniz Institute of Plant Genetics and Crop Plant Research(IPK)OT GaterslebenCorrensstraße 306466 Stadt SeelandGermany Present address:Institute of Science and Technology AustriaAm Campus 13400 KlosterneuburgAustria Present address:Institute of Molecular BiotechnologyDr.Bohr-Gasse 31030 ViennaAustria Present address:Institute of Crop Science and Resource ConservationUniversity of BonnBonnGermany 

出 版 物：《Plant Communications》 (植物通讯（英文）)

年 卷 期：2022年第3卷第2期

页      面：92-111页

核心收录：

学科分类：0710[理学-生物学] 09[农学] 0903[农学-农业资源与环境] 

基　　金：The research leading to these results received funding from the European Research Council under the European Union Seventh Framework Pro-gramme ERC-2013-STG grant agreement 335691 the Austrian Science Fund(FWF)P27818-B22,I 3033-B22 the Austrian Academy of Sciences(OEAW) the Deutsche Forschungsgemeinschaft(DFG,German Research Foundation)under Germany’s Excellence Strategy-EXC 2070-390732324 

主　　题：topless auxin pattern-triggered immunity PTI effector maize Ustilago maydis 

摘      要：In plants,the antagonism between growth and defense is hardwired by hormonal *** perception of pathogen-associatedmolecularpatterns(PAMPs)frominvadingmicroorganismsinhibits auxin signalingand plant ***,pathogens manipulate auxin signaling to promote disease,but how this hormone inhibits immunity is not fully *** maydis is a maize pathogen that induces auxin signaling in its *** characterized a *** effector protein,Naked1(Nkd1),that is translocated into the host *** its native ethylene-responsive element binding factor-associated amphiphilic repression(EAR)motif,Nkd1 binds to the transcriptional co-repressors TOPLESS/TOPLESS-related(TPL/TPRs)and prevents the recruitment of a transcriptional repressor involved in hormonal signaling,leading to the derepression of auxin and jasmonate signaling and thereby promoting susceptibility to(hemi)biotrophic pathogens.A moderate upregulation of auxin signaling inhibits the PAMP-triggered reactive oxygen species(ROS)burst,an early defense ***,our findings establish a clear mechanism for auxin-induced pathogen *** Nkd1 variants with increased expression or increased EAR-mediated TPL/TPR binding trigger typical salicylic-acid-mediated defense reactions,leading to pathogen *** implies that moderate binding of Nkd1 to TPL is a result of a balancing evolutionary selection process to enable TPL manipulation while avoiding host recognition.