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Effect of Chinese Herbal drug-containing serum for Activating-Blood and Dispelling-Toxin on ox-LDL-Induced Inflammatory Factors'Expression in Endothelial Cells

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Chinese Journal of Integrative Medicine 2012年第1期18卷 30-33页

作者：蒋跃绒缪宇杨琳薛梅郭春雨马晓娟殷惠军史大卓陈可冀 Department of Cardiovascular Diseases Xiyuan HospitalChina Academy of Chinese Medical Sciences

Objective： To investigate the effects of drug-containing serum of Chinese herbal compound, Xiongshao Capsule （芎芍胶囊, XS, for activating-blood） and Huanglian Capsule （黄连胶囊, HL, for dispellingtoxin） on the oxidized low-density lipoprotein （ox-LDL）-induced inflammatory factors in human umbilical vein endothelial cells （HUVECs）. Methods： Thirty-two rats were randomly divided into four groups： the blank control group treated with distilled water, the positive control group treated with simvastatin （1.8 mg/kg）, the test group I treated with Chinese herbal compound of XS （0.135 g/kg）, and the test group 1T treated with Chinese herbal compound of XS （0.135 g/kg） and HL （0.135 g/kg）. All the treatments were administered for 7 successive days by gastrogavage. Rats＇ blood serum was harvested 1 h after the last administration to prepare respective drug- containing serum. HUVECs were exposed to ox-LDL （100 μg/mL） to induce cell injury model and incubated with corresponding drug-containing serum for 24 h. Untreated HUVECs were set for blank control. Levels of interleukin-6 （IL-6）, tumor necrosis factor-α （TNF-α）, and soluble intercellular adhesion molecule-1 （slCAM-1） in supematant of cultured HUVECs were determined by enzyme-linked immunosorbent assay （ELISA）. HUVEC surface expressions of ICAM-1 and E-selectin were determined by flow cytometry. Results： Levels of IL-6, TNF-α, and sICAM-1 in the supernatant of HUVECs as well as the cell surface expressions of ICAM-1 and E-selectin significantly increased after 24-h ox-LDL stimulation （P〈0.01）, while the abnormal elevations, except slCAM-1 in the test group Ⅰ, were all reduced in the treated groups （the positive control and the two test groups） significantly （P〈0.01 or P〈0.05）. Besides, the effect in the test group Ⅱ seemed somewhat higher than that in the test group Ⅰ but with no statistical significance （P〉0.05）. Conclusion： drug-containing serum of XS plus HL has a certain inhibitory effect on the vascular endothelial inflammat

关键词： activating blood and dispelling toxin drug-containing serum endothelial cell oxidized low-densitylipoprotein inflammation

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A novel traditional Chinese medicine combination for radiation

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Radiation Medicine and Protection 2024年第1期5卷 37-42页

作者： Ming Shang Yicheng Ke Jiangyun Liu Zhifei Cao Liying Zhang Wenyang Zhang Weiwei Pei Guangming Zhou Yongqi Liu Provincial-Level Key Laboratory for Molecular Medicine of Major Diseases and the Prevention and Treatment with Traditional Chinese Medicine Research in Gansu Colleges and Universities Gansu University of Chinese MedicineLanzhou 730000China School of Radiation Medicine and Protection Medical College of Soochow UniversitySuzhou 215123China Collaborative Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions Suzhou 215123China College of Pharmaceutical Sciences Soochow UniversitySuzhou 215123China Department of Pathology The Second Affiliated HospitalMedical College of Soochow UniversitySuzhou 215123China

Objective:To formulate an efficacious radioprotective combination of Chinese medicines with minimal ***:Aqueous and alcoholic extracts from 38 traditional Chinese herbs were *** produce drugcontaining sera,rats received six doses of each extract via oral gavage at 12-h ***,human lung epithelial BEAS-2B cells were cultured in these drug-containing *** cell viability was assessed after different doses of irradiation to identify the radioprotective effects of Chinese herbal *** efficacy of a selected Chinese herbal extract combination was further confirmed through cell viability analysis via in vitro colony formation and survival rate assessments in C57BL/6 mice ***:Extracts from Angelicae Sinensis Radix(ASR;two extracts),Citri Reticulatae Pericarpium(CRP),Platycladi Cacumen(PC),Schisandrae chinensis Fructus(SF),Scutellariae Radix(SR),and Glycyrrhizae Radix et Rhizome(GRR)demonstrated radioprotective *** combination of the seven Chinese herbal extracts notably increased the survival and viability of the BEAS-2B cells exposed to varying doses of ***,the group of mice receiving Chinese medicine combination and irradiation exhibited prolonged survival after exposure to 6.5 Gy Xrays with a median survival of(14=2)d compared to(11=2)d in the irradiated group without the herbal ***,the Chinese medicine combination group displayed a significantly higher 28 d survival rate(30%)compared to the irradiation-only group(16.6%,P<0.05).Conclusion:The novel combination of Chinese herbal extracts from ASR,CRP,PC,SF,SR,and GRR has the potential for radiation protection applications.

关键词： Radiation protection Acute radiation syndrome Chinese medicine drug-containing serum

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Chang'an Ⅱ Decoction(肠安Ⅱ号方)-containing serum Ameliorates Tumor Necrosis Factor-α-Induced Intestinal Epithelial Barrier Dysfunction via MLCK-MLC Signaling Pathway in Rats

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Chinese Journal of Integrative Medicine 2020年第10期26卷 745-753页

作者： CHEN Ting YIN Xiao-Ian KANG Nan WANG Xiao-ge LI Bao-shuang JI Hai-jie ZHANG Yin-qiang BIAN Li-qun ZHANG Bei-hua WANG Feng-yun TANG Xu-dong Department of Gastroenterology Xiyuan HospitalChina Academy of Chinese Medical SciencesBeijing(100091)China Graduate School of China Academy of Chinese Medical Sciences Beijing(100700)China Department of Internal Medicine Jining Medical College Affiliated HospitalJining(272029)Shandong ProvineeChina Department of Gastroenterology Henan University of Chinese Medicine Affiliated HospitalZhengzhou(136300)China Department of Liver Diseases Xiyuan HospitalChina Academy of Chinese

Objective To investigate the effect of Chang’an Ⅱ Decoction(肠安Ⅱ号方))-containing serum on intestinal epithelial barrier dysfunction in *** Tumor necrosis factor(TNF)-α-induced injury of Caco-2 monolayers were established as an inflammatory model of human intestinal ***-2 monolayers were treated with blank serum and Chang’an Ⅱ Decoction-containing serum that obtained from the rats which were treated with distilled water and Chang’an Ⅱ Decoction intragastrically at doses of 0.49,0.98,1.96 g/(kg·d)for 1 week,*** preparation of containing serum,cells were divided into the normal group,the model group,the Chang’an Ⅱ-H,M,and L groups(treated with 30 ng/mL TNF-αand medium plus 10%high,middle-,and low-doses Chang’an Ⅱ serum,respectively).Epithelial barrier function was assessed by transepithelial electrical resistance(TER)and permeability of fluorescein isothiocyanate(FITC)-labeled *** electron microscopy was used to observe the ultrastructure of tight junctions(TJs).Immunofluorescence of zonula occludens-1(ZO-1),claudin-1 and nuclear transcription factor-kappa p65(NF-κBp65)were measured to determine the protein *** mRNA expression of myosin light chain kinase(MLCK)was measured by real-time polymerase chain *** expression levels of MLCK,myosin light chain(MLC)and p-MLC were determined by Western *** Chang’an Ⅱ Decoction-containing serum significantly attenuated the TER and paracellular permeability induced by TNF-α.It alleviated TNF-α-induced morphological alterations in TJ *** increases in MLCK mRNA and MLCK,MLC and p-MLC protein expressions induced by TNF-αwere significantly inhibited in the Chang’an Ⅱ-H ***,Chang’an Ⅱ Decoction significantly attenuated translocation of NF-κBp65 into the *** High-dose Chang’an Ⅱ-containing serum attenuates TNF-α-induced intestinal barrier *** underlying mechanism may be involved in inhibiting the MLCK-MLC ph

关键词： myosin light chain kinase-myosin light chain signaling pathway intestinal epithelial cells tight junction tumor necrosis factor-α Chang’an II Decoction drug-containing serum

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How do Chinese medicines that tonify the kidney inhibit dopaminergic neuron apoptosis?

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Neural Regeneration Research 2013年第30期8卷 2820-2826页

作者： Shaogang Lin Shuifen Ye Jinmu Huang Yun Tian Yihui Xu Mengqi Wu Jingxia Wang Songying Wu Jing Cai Hospital of Putian University Academy of Integrative Medicine Fujian University of Traditional Chinese Medicine College of Pharmaceutical Sciences Zhejiang University The Second People’s Hospital of Fujian University of Traditional Chinese Medicine

Wistar rats were intragastrical y perfused with Chinese medicines used for tonifying the kidney. These included 0.180 g/mL of Herba Epimedi （Epimedium）, Semen Cuscutae （Dodder Seed）, or Herba Cistanches （Desertliving Cistanche）, 0.04 mg/mL monoamine oxidase-B inhibitor selegiline, or distil ed water for 14 consecutive days to prepare drug-containing serum or blank serum. MES23.5 cells in the logarithmic phase were cultured in media supplemented with 15%drug-containing serum for 24 hours, fol owed by incubation in culture solution containing 100μmol/L H2O2 for 3 hours. 3-（4,5-Dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide （MTT） and flow tometry results showed that al drug-containing serums improved the survival rate of H 2 O 2-injured MES23.5 cells, inhibited pro-apoptotic FasL and caspase-3 expression, promoted anti-apoptotic Bcl-2 expression. However, drug-containing serums had little influence on Fas expression in H 2 O 2-injured MES23.5 cells. Enzyme-linked immunosorbent assay results showed that serum containing Herba Cistanches or Herba Epimedi increased the expression of nerve growth factor, brain-derived neurotrophic factor, and glial cellline-derived neurotrophic factor in injured MES23.5 cells;serum containing Semen Cuscutae only increased brain-derived neurotrophic factor expres-sion; while expression of the above neurotrophic factors remained the same in cells treated with serum containing selegiline. These findings indicate that Chinese medicines used to tonify the kid-ney can protect nerve cells by regulating the expression of apoptosis-related factors and neuro-trophic factors in MES23.5 cells.

关键词： neural regeneration traditional Chinese medicine drug-containing serum MES23.5 dopaminergicnerve cells neurotrophic factors apoptosis factors Parkinson's disease neuroprotection

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Experimental Study on the Suppression of Sodium Nitroprussiate-lnduced Chondrocyte Apoptosis by Tougu Xiaotong Capsule(透骨消痛胶囊)-containing serum

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Chinese Journal of Integrative Medicine 2011年第6期17卷 436-443页

作者：李西海吴明霞叶蕻芝陈文列林久茂郑良朴刘献祥 Academy of Integrative Medicine of Fujian University of Traditional Chinese Medicine Fujian Provincial Key Lab for Integrative Medical Study on Senile Diseases

Objective：To study the mechanism of action of Tougu Xiaotong Capsule（透骨消痛胶囊,TGXTC） ex vivo in suppressing chondrocyte（CD） apoptosis induced by sodium nitroprussiate（SNP）.Methods：Thirty New Zealand rabbits,2 months old,were randomized by lottery into five groups,six in each：the blank group treated with saline,the positive control group treated with Zhuanggu Guanjie Pill（壮骨关节丸,70 mg/kg）,and the three experimental groups,EGA,EGB,and EGC,treated with low dose（35 mg/kg）,moderate dose（70 mg/kg）,and high dose（140 mg/kg） of TGXTC,*** treatments were administered via gastrogavage twice a day for 3 *** blood was collected from the abdominal aorta and drug or drug metabolites-containing serum was *** obtained from knee joints of 16 four-week-old New Zealand rabbits were cultured to the third passage and confirmed by toluidine blue *** of various final concentrations（0,0.5,1.0,and 2.0 mmol/L） was used to induce CD apoptosis,and the dosage-effect relationship of SNP in inducing CD apoptosis was *** samples from the blank,control,and three dosages of TGXTC-treated rabbits were tested in the CD culture in the presence of *** apoptosis was determined by Hoechst 33342 staining,viability of CDs was quantified by MTT,CD apoptosis rate was determined by annexin V-FITC/PI staining,levels of p53 and Bcl-2 mRNA expression in CDs were determined with RT-PCR,and contents of caspase-3 and caspase-9 proteins were determined by ***：CD apoptosis was induced by SNP at all concentrations tested and in a dose-dependent *** SNP concentration of 1 mmol/L and treatment duration of 24 h appeared to be optimal and were selected for the *** samples from the positive control rabbits and from the two higher doses of TGXTC-treated rabbits showed reduction of SNP-induced CD apoptosis,decrease in p53 mRNA expression,inhibition of catalytic activities of caspase-3 and caspase-9,and increase in Bcl-2 mRNA expression when

关键词： osteoarthritis drug-containing serum chondrocyte cell apoptosis Tougu Xiaotong Capsule osteoarthritis drug-containing serum chondrocyte cell apoptosis Tougu Xiaotong Capsule osteoarthritis drug-containing serum chondrocyte cell apoptosis Tougu Xiaotong Capsule

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