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Journal of Behavioral and Brain Science 2018年第3期8卷 117-125页

作者： William T. Prater Malli Swamy Megan D. Beane Deranda B. Lester Department of Psychology The University of Memphis Memphis TN USA

Carbon fiber microelectrodes (CFEs) are useful when combined with electrochemical techniques for measuring changes in neurotransmitter concentrations. We addressed conflicting details regarding the use of CFEs. Experimental groups consisted of CFEs at different ages (1 week, 1 month, or 2 months), cleaned in solvents (isopropanol or xylene), and exposed to in vitro use (flow cell calibrations) or in vivo use (in brain tissue). In order to determine if any of these factors affect CFE sensitivity, the present study utilized fixed potential amperometry and a flow injection system to calibrate CFEs for the measurement of Dopamine. The sensitivity index (nA/μM per 100 μm of exposed carbon fiber) was not affected by the age of CFEs or pre-cleaning with xylene or isopropanol. CFE sensitivity of the in vitro exposure group also did not differ from untreated CFEs, indicating the calibration process did not alter sensitivity. However, in vivo use in brain tissue did reduce sensitivity. This effect was negated and sensitivity restored by cleaning CFEs in isopropanol or xylene following in vivo brain recordings. Given that variations in CFE sensitivity can skew results, our findings can help standardize CFE use and explain discrepancies between researchers.

关键词： Fixed Potential Amperometry Carbon Fiber Electrode Dopamine Flow Injection System Electrode Calibration

Retinal Dopamine levels in both eyes are equally reduced in CBA/Ca J mice treated with monocular form deprivation

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Retinal dopamine levels in both eyes are equally reduced in ...

Electrochemical Determination of Epinephrine with a Pyrolytic Graphite Electrode in the Presence of Ascorbic Acid and Dopamine

中国神经科学学会第十二届全国学术会议

作者： Kang-Wei QIAN Yun-Yun LI Xiao-Hua WU Yong-Mei ZHONG Xiong-Li YANG Shi-Jun WENG Institutes of Brain Science State Key Laboratory of Medical Neurobiology and Collaborative Innovation Center for Brain Science Fudan University

Objective Unlike observed in most other species, retinal Dopamine（DA） levels are unaltered in C57 BL/6 mice with form-deprivation myopia（FDM）. Although implying a novel, DA-independent mechanism for FDM, this discrepancy might be attributed to the fact that the C57 BL/6 strain is deficient in melatonin, a hormone that interplays with DA. Here we explored whether and how form deprivation changed refractive development and retinal DA levels in a mouse strain proficient in melatonin. Methods CBA/Ca J mice were used in this study. Normal refractive development was measured every 5-10 days from P21 to P106. FDM was induced by wearing monocular occluder, starting at P21, for 5 weeks. Refractions were measured with an eccentric infrared photorefractor. Ocular biometry was performed with A-scan ultrasonography, optical coherence tomography and photokeratometry. Retinal DA, DOPAC and vitreal DOPAC（an indicator of DA release） levels were assessed by HPLC analysis. Retinal tyrosine hydroxylase（TH） levels were examined by quantitative Western blotting. Results Under normal visual conditions, the refraction at P21 was 4.544 ± 0.147 D. It increased continuously in hyperopic direction, reaching a peak（18.262 ± 0.378 D） at P86, and then leveled off. FDM was successfully induced with the refractive status of deprived eyes（8.489 ± 0.877 D） being significantly different（P = 0.00000003） from fellow eyes（13.048 ± 0.870 D）. As expected, axial length in deprived eyes（2.698 ± 0.007 mm） is significantly longer（P = 0.0011） than that in fellow eyes（2.675 ± 0.008 mm）;but unexpectedly, both values were significantly smaller（P = 0.0012, 0.000001） than that of normal eyes（2.736 ± 0.006 mm） in intact animals. Consistent with previous results in C57 BL/6 mice, either retinal DA（P = 0.234）, DOPAC（P = 0.643） or TH（P = 0.653） levels were similar between deprived and fellow eyes. But surprisingly, both deprived and fellow eyes exhibited significantly lower retinal DA（P = 0.006, 0.0004）, DOPAC（P = 0.00002, 0.00005） and TH（P = 0.015, 0.029） levels tha

关键词： form-deprivation myopia melatonin Dopamine refraction CBA/CaJ mouse

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Chinese Chemical Letters 2006年第5期17卷 657-660页

作者： Xiu Hui LIU Yan ZHANG Guo Fang ZUO Xiao Quan LU College of Chemistry ＆ Chemical Engineering Northwest Normal University Lanzhou 730070

A method for determination of epinephrine（EP） in the presence of ascorbic acid （AA） and Dopamine （DA） with bare pyrolytic graphite electrodes has been described for the first time. In pH 7.0 phosphate buffer solution, the linear relationship was observed between the reduction peak current of EP and its concentration over the range from 1×10^-4 to 5×10^-7 mol/L, the related coefficient is 0.9992 （N=8）.

关键词： Differential pulse voltammetry epinephrine ascorbic acid Dopamine pyrolytic graphite electrodes

Electrochemical Behaviour of Dopamine at Three-Dimensional Au Nanoparticles（NPs）/Polyelectrolyte Multilayer Hollow Nanofibers（HNFs） Modified Boron-Doped Diamond（BDD） Electrode

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Electrochemical Behaviour of Dopamine at Three-Dimensional A...

Effect of Dopamine neurotransmitter expression on sleep disorders in neurological inpatients

中国第四届静电纺丝大会(CICE2016)

作者： Chao Pan Feixu Zong Jingyi Gao College of Science Dalian Ocean University

Gold nanoparticles（Au NPs） and a polyelectrolyte（PE） have been assembled alternately on a polystyrene（PS） nanofibers（NFs） by a layer-by-layer（LBL） self-assembly technique to form three-dimensional（3D）（Au/PAH）4/（PSS/PAH）4 multilayer coated PS hollow nanofibers（HNFs）. The Au NPs/PE multilayer HNFs have been used to modify a boron-doped diamond（BDD） electrode. The electrochemical behaviors of Dopamine（DA） on the bare and modified BDD electrode are studied. The cyclic voltammetric studies indicate that the negatively charged, three-dimensional Au NPs/PE multilayer HNFs-modified BDD electrodes show enhanced electrocatalytic behavior for the oxidation of DA. The oxidation peak potential of DA shifted negatively to 0.308 V and the oxidation current increased by about 62% on Au NPs/PE HNFs modified ABDD electrode.

关键词： Electrospun Hollow nanofibers Boron-doped diamond（BDD） electrode Electrochemistry Dopamine

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Journal of Hainan Medical University 2019年第11期25卷 28-32页

作者： Xian-Jing Tang Ling Ding Neurology Department Changsha Third HospitalChina

Objective:To explore the effect of Dopamine neurotransmitter expression on sleep disorders in neurological inpatients.Methods: Thirty-four patients with sleep disorders and 30 volunteers from February 2018 to November 2018 in the neurology department of our hospital were selected as the study subjects. They were divided into the observation group and the control group. 64 participants stopped taking stimulating drinks such as coffee and alcohol two weeks before the experiment. The sleep quality and depression were measured by the Pittsburgh Sleep Quality Index (PSQI) and Hamilton Depression Scale (HAMD). Degree of depression was examined by brain MRI. Two days later,11C-Raclopril labeled brain Dopamine neurotransmitter binding to receptors was scanned by PET/CT. SPSS was used to analyze the correlation between Dopamine neurotransmitters and sleep disorders.Results: The HAMD and PSQI scores of the observation group were significantly higher than those of the control group (PDopamine binding ability of nucleus dorsal caudate putamen, caudate nucleus and putamen in the observation group was significantly lower than that in the control group (P0.05).Conclusion: The decrease of Dopamine receptor binding in patients with sleep disorders is related to the course of sleep disorders, suggesting that the decrease of Dopamine expression and sleep disorders have clinical significance.

关键词： Brain Dopamine Sleep disorder Nervous system

Mechanism of Buyin Qianzheng Fang on preventing Dopamine neuron from apoptosis by regulating intracellular Ca^(2+) in PD Mice

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解剖学杂志 2021年第S1期44卷 228-229页

作者： Zhao Haihong Feng Wandi Ma Haojie Cheng Cuicui Zhou Mengqi Gao Yushan Gai Cong Sun Hongsun Department of Anatomy Beijing University of Chinese MedicineBeijing 100029

The purpose of this study is to investigate the possible protective mechanism of Buyin Qianzheng Fang(BYQZF)on endoplasmic reticular and mitochondrial function through intracellular Ca^(2+) to protect Dopamine neurons in Parkinson's disease mice model.C57BL/6 mice were randomly divided into normal group,model group,BYQZF group.The behavior of mice was observed by climbing and beam hang tests.

关键词： C57BL/6 Dopamine protective

Study on the Expression of Dopamine Receptor-3(DRD3) in Rats after Sacral Spinal Cord Transection

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Medicinal Plant 2019年第1期10卷 62-65页

作者： Ying CHEN Xiang ZHANG Meng CHEN Xiaoguang WU Huanna TIAN Shufang MAO Xin MENG Liqun REN Chengde Medical College

[Objectives] This study aimed to study the distribution characteristics of DRD3(Dopamine receptor-3) and the changes in its expression before and after spinal cord injury(SCI), in order to lay a morphological basis for later research. [Methods] Adult male Wistar rats were randomly divided into sham operation group and SCI group. The rat spinal cord transection model at the sacral 2(S_2) segment was established. Rat tail spasticity score was performed 60 d after SCI, and the rats with 4-5 points were screened for perfusion. The expression of DRD3 in the sacral spinal cord(S+C segment) was detected by immunofluorescence. [Results] In normal rats, DRD3 was mainly distributed in the dorsal horn(DH), intermediate zone(IMZ) and ventral horn(VH) of the gray matter. It was also expressed in the white matter of the spinal cord. After SCI, the distribution of DRD3 in the segment below the injury section was similar to that of normal rats. However, the expression was different(P<0.05). [Conclusions] There was no significant change in the distribution of DRD3 in spinal cord after SCI. After the spinal cord S_2 was completed transected, the expression of DRD3 was significantly reduced in the DH, IMZ and VH regions of the gray matter of the spinal cord.

关键词： Spinal cord injury(SCI) Dopamine receptor-3(DRD3) Aromatic amino acid decarboxylase

Capillary electrophoresis with inhibited electrochemiluminescent detection for the trace analysis of epinephrine and Dopamine

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Journal of Pharmaceutical Analysis 2010年第2期22卷 77-82页

作者： Xue-Yang Xiao,Long-Hua Guo,Bin Qiu,Zhen-Yu Lin,Guo-Nan Chen Ministry of Education Key Laboratory of Analysis and Detection for Food Safety,Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety,Department of Chemistry,Fuzhou University,Fuzhou 350002,China. MinistryofEducationKeyLaboratoryofAnalysisandDetectionforFoodSafety FujianProvincialKeyLaboratoryofAnalysisandDetectionforFoodSafetyDepartmentofChemistryFuzhouUniversityFuzhou350002China

In this paper,a novel electrochemiluminescent (ECL) detection approach was developed for highly sensitive detection of ECL inhibitors based on the ECL inhibition of Ru(bpy)32+/2-(Dibutylamino)ethanol (DBAE) system. A microfluidic ECL detection cell was fabricated to couple with the capillary electrophoresis system,the electrochemical system and the postcolumn injection system. Both Ru(bpy)32+ and DBAE solutions were injected directly to the working electrode surface by a micro-infusion system to obtain a high and stable ECL signal. The performance of this setup was demonstrated by the analysis of two typical ECL inhibitors,Dopamine and epinephrine. Under the optimal conditions,the limit of detection (LOD) for Dopamine and epinephrine was 50nM and 5nM respectively. The proposed method was also successfully used for the trace analysis of Dopamine and epinephrine in human serum samples.

关键词： capillary electrophoresis inhibited electrochemiluminescence Ru(bpy)32+ epinephrine Dopamine

α-Cyclodextrin Incorporated Carbon Nanotube-coated Electrode for the Simultaneous Determination of Dopamine and Epinephrine

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Chinese Journal of Chemistry 2005年第3期23卷 297-302页

作者：王歌云刘秀娟罗国安王宗花 DepartmentofChemistry JiangxiInstituteofEducationNanchangJiangxi330029China DepartmentofChemistry TsinghuaUniversityBeijing100084China DepartmentofChemistry JiangxiInstituteofEducationNanchangJiangxi330029China//DepartmentofChemistryTsinghuaUniversityBeijing100084China

An α‐cyclodextrin (α‐CD) incorporated carbon nanotube (CNT)‐coated electrode was fabricated and applied to the simultaneous determination of Dopamine (DA) and epinephrine (EP). It has been found that the modified electrode shows strong catalytic effects on the electro‐separation of DA and EP, and the cathodic potential difference between DA and EP is about 390 mV. The reducing peak current is proportional to DA and EP concentrations in the range of 2.0×10 ‐6 –1.0×10 ‐3 and 1.0×10 ‐6 –1.0×10 ‐3 mol·L ‐1 , respectively. Their detection limits can reach 1×10 ‐6 and 5×10 ‐7 mol·L ‐1 , respectively. Because the oxidation of ascorbic acid (AA) is an irreversible reaction in α‐CD/CNT film, the interference of AA in determination of DA and EP is eliminated.

关键词： Dopamine epinephrine α‐cyclodextrin carbon nanotube‐coated electrode

Effect of electroacupuncture preconditioning on the contents of Dopamine and 5-hydroxytryptamine in lateral hypothalamus area and cerebellar fastigial nucleus of rats with myocardial ischemia-reperfusion injury

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China Medical Abstracts(Internal Medicine) 2021年第3期38卷 132-133页

作者：余情 Acupunct&Moxibust&Tuina Coll Anhui CM UnivHefei 230012

Objective To observe the effect of electroacupuncture(EA)preconditioning at heart meridian acupoints on the contents of Dopamine(DA)and 5-hydroxytryptamine(5-HT)in lateral hypothalamus area(LHA)and cerebellar fastigial nucleus(FN)in the rats with acute myocardial ischemia-reperfusion injury(MIRI),and explore the role and mechanism of LHA and FN in the effect of EA at heart meridian acupoints against acute MIRI.

关键词： acute Dopamine ischemia