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diversity analysis of acute hepatopancreatic necrosis disease-positive Vibrio parahaemolyticus strains

Aquaculture and Fisheries 2017年第6期2卷 278-285页

作者： Bo Feng Haiquan Liu Meng Wang Xiaohong Sun Yingjie Pan Yong Zhao College of Food Science and Technology Shanghai Ocean UniversityShanghai 201306China Shanghai Engineering Research Center of Aquatic-Product Processing&Preservation Shanghai 201306China Laboratory of Quality&Safety Risk Assessment for Aquatic Products on Storage and Preservation(Shanghai) Ministry of AgricultureShanghai 201306China

Acute hepatopancreatic necrosis disease(AHPND),also called early mortality syndrome(EMS),has caused massive economic loss in aquaculture since *** was considered that the disease was caused by a novel type of Vibrio parahaemolyticus which harbors a large *** insecticidal Photorhabdus insect-related(pir)genes were found in the *** this study,81 isolates have been collected from a shrimp farm in Maoming,Guangdong Province,*** isolates were identified using 3 pairs of primer recently made *** the 16S rRNA gene and pir gene were ***-PCR was employed for analysis of strain diversity.12 isolates were amplified by PCR and identified as Vibrio parahaemolyticus by 16S rRNA sequence *** alignments of the pir gene showed few mutations in the 12 strains of *** 12 strains separated into 3 clusters at a cut of 90%similarity by *** virulence gene expression and toxicity profile showed differences between *** study generated comprehensive identification of pathogenic bacteria and microbial molecular resources,which will contribute to future research of AHPND.

关键词： Acute hepatopancreatic necrosis disease Challenge assay diversity analysis

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Association between childhood obesity and gut microbiota:16S rRNA gene sequencing-based cohort study

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World Journal of Gastroenterology 2024年第16期30卷 2249-2257页

作者： Xu-Ming Li Qing Lv Ya-Jun Chen Lu-Biao Yan Xin Xiong Laboratory Department Nanjing Medical University Affiliated Obstetrics and Gynecology Hospital(Nanjing Maternal and Child Health Hospital)Nanjing 210004Jiangsu ProvinceChina Department of Pediatrics Shenzhen University General HospitalShenzhen 518055Guangdong ProvinceChina Department of Inspection Division Women’s Hospital of Nanjing Medical University(Nanjing Maternity and Child Health Care Hospital)Nanjing 210004Jiangsu ProvinceChina Department of Pediatrics Women’s Hospital of Nanjing Medical University(Nanjing Maternity and Child Health Care Hospital)Nanjing 210004Jiangsu ProvinceChina Department of Neonatology Chenzhou First People’s HospitalChenzhou 423000Hunan ProvinceChina

BACKGROUND This study aimed to identify characteristic gut genera in obese and normal-weight children(8-12 years old)using 16S rDNA *** research aimed to provide insights for mechanistic studies and prevention strategies for childhood *** normal-weight and thirty age-and sex-matched obese children were *** and body measurements were collected,and fecal samples underwent 16S rDNA *** differences in body mass index(BMI)and body-fat percentage were observed between the *** of gut microbiota diversity revealed lowerα-diversity in obese *** in gut microbiota composition were found between the two *** and Firmicutes were more abundant in the obese group,while Bacteroides and Sanguibacteroides were more prevalent in the control *** To identify the characteristic gut genera in obese and normal-weight children(8-12-year-old)using 16S rDNA sequencing,and provide a basis for subsequent mechanistic studies and prevention strategies for childhood *** Thirty each normal-weight,1:1 matched for age and sex,and obese children,with an obese status from 2020 to 2022,were included in the control and obese groups,*** information was collected through questionnaires and body measurements were obtained from both obese and normal-weight *** samples were collected from both groups and subjected to 16S rDNA sequencing using an Illumina MiSeq sequencing platform for gut microbiota diversity *** Significant differences in BMI and body-fat percentage were observed between the two *** Ace and Chao1 indices were significantly lower in the obese group than those in the control group,whereas differences were not significant in the Shannon and Simpson ***-Wallis tests indicated significant differences in unweighted and weighted UniFrac distances between the gut microbiota of normal-weight and obese children(P<0.01),suggest

关键词： Childhood obesity Gut microbiota 16S rDNA sequencing diversity analysis Genus identification Body mass index

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A high-efficiency pretreatment method for elution of pathogenic bacteria in lettuce

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Food Quality and Safety 2022年第4期6卷 588-596页

作者：廖小艳普春敏崔妍白亚龙史贤明陈丽丽 Department of Public Health Laboratory Sciences College of Public HealthHengyang Medical SchoolUniversity of South ChinaHengyangHunanChina Institute for Agri-food Standards and Testing Technology Shanghai Academy of Agricultural SciencesShanghaiChina MOST-USDA Joint Research Center for Food Safety&Bor Luh Food Safety Center Department of Food Science and EngineeringSchool of Agriculture and BiologyShanghai Jiao Tong UniversityShanghaiChina

Many current studies on rapid detection of pathogenic bacteria in foods have focused on the construction of detection methods,neglecting *** is also a key step to efficiently elute pathogenic bacteria from food samples for rapid detection and can even determine the success or failure of an *** this study,we used Escherichia coli(***),Salmonella enteritidis(***),and Listeria monocytogenes(***)as model bacteria to compare the elution efficiency of different eluants;explore the effect of surfactant,ionic strength,protein(or amino acid and peptide),and enzyme on the recovery rate of bacteria in lettuce;and compare the compound effect caused by multiple ***,we developed an efficient bacterial recovery method and confirmed the superiority of this method to analyze the bacterial diversity of eluants from *** results showed that the recovery efficiency of ***,***,and ***,which were artificially contaminated with approximately 10^(5)CFU/g in lettuces,could reach 94.4%,90.6%,and 93.7%by using 10 mmol/L Tris·HCl(pH 9.5)with 0.1%peptone and 300 U/100 mL of cellulase,and furthermore,the elution efficiency could reach 99.6%,98.6%,and 100%with the aid of a 2-min *** the lettuce samples with only native bacteria,the recovery rate reached 92.1%for viable aerobic bacteria by this method,which was approximately 10%higher than that of the modified previous *** bacterial diversity of the eluted solution was analyzed,and the result was significantly *** these advantages,it is important to improve the elution efficiency to achieve rapid and accurate detection of pathogenic bacteria in lettuces.

关键词： Lettuce foodborne pathogens elution pretreatment diversity analysis

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Development of a Highly Informative Microsatellite (SSR) Marker Framework for Rice (Oryza sativa L.) Genotyping

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Agricultural Sciences in China 2010年第12期9卷 1697-1704页

作者： JIANG Shu-kun HUANG Cheng ZHANG Xi-juan WANG Jia-yu CHEN Wen-fu XU Zheng-jin Key Laboratory of Crop Physiology Ecology Genetics and Breeding Ministry of Agriculture/Key Laboratory of Northern Japonica Rice Breeding of Liaoning Province Shenyang Agricultural University Shenyang 110086 P.R. China Dandong Academy of Agricultural Sciences Dandong 118109 PR. China

To select highly informative microsatellite markers （SSRs） and establish a useful genetic SSR framework for rice genotyping, 15 rice （Oryza sativa L.） cultivars including six indica varieties and nine japonica varieties were used to analyze the polymorphism information content （PIC） value of 489 SSR markers. A total of 1 296 alleles were detected by 405 polymorphic markers with an average of 3.2 per locus. The PIC value of each chromosome was ranged from 0.4039 （chromosome 2） to 0.5840 （chromosome 11）. Among the two rice subspecies, indica （0.3685-0.4952） gave a higher PIC value than japonica （0.1326-0.3164） and displayed a higher genetic diversity. Genetic diversity of indica was high on chromosome 12 （0.4952） and low on chromosome 8 （0.3685）, while that for japonica was high on chromosome 11 （0.3164） and low on chromosome 2 （0.1326）. A SSR framework including 141 highly informative markers for genotyping was selected from 199 SSR markers （PIC〉0.50）. Ninety-three SSR markers distributed on 12 chromosomes were found to be related to indica-japonica differentiation. Of these 93 pairs of SSR primers, 17 pairs were considered as core primers （all the japonica varieties have the same specific alleles, while the indica varieties have another specific alleles）, 48 pairs as the second classic primers （all the japonica or indica varieties have the same specific alleles, while the indica or japanica varieties have two or more other specific alleles ） and 28 pairs as the third classic primers （all the japonica and indica varieties have two or more alleles, but the specific alleles are different between japonica and indica）. Thirty-two SSR markers were selected to be highly informative and useful for genetic diversity analysis of japonica varieties. This work provides a lot of useful information of SSR markers for rice breeding programs, especially for genotyping, diversity analysis and genetic mapping.

关键词： SSR marker core primers rice variety differentiation diversity analysis genetic mapping

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Variability and divergence in Pongamia pinnata for further use in tree improvement

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Journal of Forestry Research 2011年第2期22卷 193-200页

作者： B.N.Divakara Rameshwar Das Institute of Forest Productivity Indian Council of Forestry Research and Education

A total of 24 candidate plus trees（CPTs） of Pongamia pinnata（L.） *** selected to elucidate their variation and diversity based on thirteen quantitative traits（4 pod traits,6 seed traits of parent trees and 3 progeny traits） at Forest Research Centre,Institute of Forest Productivity-Mandar,Ranchi district during *** results show that,CPT-19 had maximum for seven traits viz,pod length（65.6 mm）,100-pod weight（542.4 g）,seed 2D（two dimension） area（351.2 mm2）,seed length（27.9 mm）,seed breadth（17.4 mm）,100-seed weight（217.9 g） and plant height（164.3 cm）.The traits,100-pod weight and 100-seed weight had a high heritability（98.4%,96.9%） accompanied with high genetic advance（46.0%,34.9%）.There is a positive significant correlation between 100-pod weight and 100-seed weight traits at both genotypic and phenotypic levels with plant height,collar diameter and volume index at 30 MAS（months after sowing）.Volume index expressed a moderate heritability（47.4%） accompanied with high genetic advance（48.4%）,indicating that the character is governed by additive gene *** divergence study,24 accessions were grouped into 6 clusters on the basis of non-hierarchical euclidian cluster *** genotypes in cluster IV（CPT-5,CPT-6,CPT-7,CPT-12,CPT-16,CPT18,CPT-22） and cluster III（CPT-4,CPT-8,CPT-9,CPT-20,CPT-21） were most heterogeneous and can be best used within group *** wide diversity exists between the cluster V and II,followed by cluster II and I and crosses between CPTs of these clusters may result in substantial *** is revealed that the existence of substantial variation and diversity can be utilized for genetic resource conservation and further tree improvement programmers of the species.

关键词： Pongamia pinnata heritability genetic advance correlation path analysis image analyzer diversity analysis

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