Association between childhood obesity and gut microbiota:16S rRNA gene sequencing-based cohort study

作     者：Xu-Ming Li Qing Lv Ya-Jun Chen Lu-Biao Yan Xin Xiong 

作者机构：Laboratory DepartmentNanjing Medical University Affiliated Obstetrics and Gynecology Hospital(Nanjing Maternal and Child Health Hospital)Nanjing 210004Jiangsu ProvinceChina Department of PediatricsShenzhen University General HospitalShenzhen 518055Guangdong ProvinceChina Department of Inspection DivisionWomen’s Hospital of Nanjing Medical University(Nanjing Maternity and Child Health Care Hospital)Nanjing 210004Jiangsu ProvinceChina Department of PediatricsWomen’s Hospital of Nanjing Medical University(Nanjing Maternity and Child Health Care Hospital)Nanjing 210004Jiangsu ProvinceChina Department of NeonatologyChenzhou First People’s HospitalChenzhou 423000Hunan ProvinceChina 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2024年第30卷第16期

页      面：2249-2257页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

主　　题：Childhood obesity Gut microbiota 16S rDNA sequencing Diversity analysis Genus identification Body mass index 

摘      要：BACKGROUND This study aimed to identify characteristic gut genera in obese and normal-weight children(8-12 years old)using 16S rDNA *** research aimed to provide insights for mechanistic studies and prevention strategies for childhood *** normal-weight and thirty age-and sex-matched obese children were *** and body measurements were collected,and fecal samples underwent 16S rDNA *** differences in body mass index(BMI)and body-fat percentage were observed between the *** of gut microbiota diversity revealed lowerα-diversity in obese *** in gut microbiota composition were found between the two *** and Firmicutes were more abundant in the obese group,while Bacteroides and Sanguibacteroides were more prevalent in the control *** To identify the characteristic gut genera in obese and normal-weight children(8-12-year-old)using 16S rDNA sequencing,and provide a basis for subsequent mechanistic studies and prevention strategies for childhood *** Thirty each normal-weight,1:1 matched for age and sex,and obese children,with an obese status from 2020 to 2022,were included in the control and obese groups,*** information was collected through questionnaires and body measurements were obtained from both obese and normal-weight *** samples were collected from both groups and subjected to 16S rDNA sequencing using an Illumina MiSeq sequencing platform for gut microbiota diversity *** Significant differences in BMI and body-fat percentage were observed between the two *** Ace and Chao1 indices were significantly lower in the obese group than those in the control group,whereas differences were not significant in the Shannon and Simpson ***-Wallis tests indicated significant differences in unweighted and weighted UniFrac distances between the gut microbiota of normal-weight and obese children(P0.01),suggest