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distal-less homeobox genes of insects and spiders： genomic organization, function, regulation and evolution

Insect Science 2016年第3期23卷 335-352页

作者： Bin Chen William H. Piel Antonia Monteiro Institute of Entomology and Molecular Biology College of Life Sciences Chongqing Normal University Chongqing 401331 P.R. China Yale-NUS College Singapore Department of Biological Sciences National University of Singapore Singapore

The distal-less （DlI） genes are homeodomain transcription factors that are present in most Metazoa and in representatives of all investigated arthropod groups. In Drosophila, the best studied insect, Dll plays an essential role in forming the proximodistal axis of the legs, antennae and analia, and in specifying antennal identity. The initiation of Dll expression in clusters of cells in mid-lateral regions of the Drosophila embryo represents the earliest genetic marker of limbs. Dll genes are involved in the development of the peripheral nervous system and sensitive organs, and they also function as master regulators of black pigmentation in some insect lineages. Here we analyze the complete genomes of six insects, the nematode Caenorhabditis elegans and Homo sapiens, as well as multiple Dll sequences available in databases in order to examine the structure and protein features of these genes. We also review the function, expression, regulation and evolution of arthropod Dll genes with emphasis on insects and spiders.

关键词： distal-less evolution function genomic organization regulation

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Increased expression of DLX2 correlates with advanced stage of gastric adenocarcinoma

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World Journal of Gastroenterology 2013年第17期19卷 2697-2703页

作者： Peng Tang Hua Huang Jiang Chang Gong-Fang Zhao Ming-Liang Lu Yan Wang Department of Gastroenterology the Second Affiliated Hospital of Kunming Medical University Department of Pathology the Second Affiliated Hospital of Kunming Medical University

AIM:To investigate the expression of distal-less homeobox 2 (DLX2) in gastric adenocarcinoma and its clinicopathological significance. METHODS:Gastric adenocarcinoma tissues were obtained from gastrectomy specimens of 129 patients from the Department of Surgery and Pathology, the Second Affiliated Hospital of Kunming Medical University. Sixty cases of normal gastric tissues were collected from gastrectomy specimens of adjacent gastric cancer margins greater than 5 cm. Patient diagnosis was established pathologically, and no patient had received chemotherapy or radiotherapy before surgery. All tissue specimens were formalin-fixed and paraffin-embedded. Immunohistochemistry was carried out to investigate the expression of DLX2 in 129 gastric adenocarcinoma tissues and 60 adjacent normal tissues. The immunos-taining reaction was semiquantitatively evaluated based on the proportion of positive cells and the median staining intensity in normal gastric epithelial cells or tumor cells. All patients had follow-up records for more than 5 years. Correlations of DLX2 expression with clinicopathological features and prognosis of patients with gastric adenocarcinoma were analyzed. All statistical analyses were performed using the SPSS 17.0 software. RESULTS:The positive expression of DLX2 was detected in 68 (52.7%) cases of 129 gastric adenocarcinoma tissues and 14 (23.3%) cases of 60 adjacent normal tissues. The difference in DLX2 expression between gastric adenocarcinoma tissues and adjacent normal tissues was statistically significant (Ⅹ2 = 14.391, P < 0.001). Moreover, high expression of DLX2 was detected in 48 (37.2%) cases of 129 human gastric cancer tissues, but not in adjacent normal tissues. The expression of DLX2 correlated with the size of tumor (P = 0.001), depth of invasion (P = 0.008), lymph node metastasis (P = 0.023) and tumor-node-metastasis stages (P = 0.020), but was not correlated with age, gender, histological differentiation and distant metastasis. The Kapl

关键词： Gastric adenocarcinoma distal-less homeobox 2 Immunohistochemistry Invasion Metastasis Prognosis

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异位表达Dichaete对果蝇足部发育的影响及其作用机制

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昆虫学报 2018年第2期61卷 149-155页

作者：董玮孔悦马恩波张建珍张敏张徐波山西大学应用生物学研究所太原030006 山西大学生命科学学院太原030006

【目的】果蝇Dichaete基因是Sox家族B亚家族基因,其表达贯穿整个胚胎发育阶段,在个体发育过程中发挥重要作用。Dichaete在野生型果蝇幼虫足芽上也有微弱表达,然而其在足发育过程中的作用少有报道。本研究旨在研究异位表达Dichaete对果... 详细信息

【目的】果蝇Dichaete基因是Sox家族B亚家族基因,其表达贯穿整个胚胎发育阶段,在个体发育过程中发挥重要作用。Dichaete在野生型果蝇幼虫足芽上也有微弱表达,然而其在足发育过程中的作用少有报道。本研究旨在研究异位表达Dichaete对果蝇足部形态构成的影响,探索相关的作用机制。【方法】黑腹果蝇Drosophila melanogaster en-Gal4品系雄性成虫与UAS-Dichaete转基因品系处女蝇杂交,驱动Dichaete异位表达,解剖子一代成虫足,在显微镜下观察异位表达的Dichaete对成虫足形态结构的影响;同时采用免疫组化技术检测异位表达Dichaete对果蝇足芽细胞凋亡和distal-less(Dll)表达的影响。【结果】异位表达Dichaete导致黑腹果蝇成虫足末端结构缺陷,引起3龄幼虫足芽细胞凋亡增加,上调了distal-less基因的表达,但对Wg和Dpp信号均无影响;抑制细胞凋亡也无法拯救该缺陷。【结论】异位表达Dichaete导致黑腹果蝇成虫足末端结构缺陷,Dichaete可能通过上调distal-less基因影响足部发育。

关键词：黑腹果蝇 Dichaete 异位表达足细胞凋亡 distal-less

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