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Salivary C-reactive protein and mean platelet volume as possible diagnostic markers for late-onset neonatal pneumonia

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World Journal of Clinical Pediatrics 2024年第1期13卷 41-51页

作者： Wafaa Ahmed Metwali Abdelrahman Mohamed Elmashad Sahar Mohey Eldin Hazzaa Mohammed Al-Beltagi Mohamed Basiony Hamza Department of Pediatric Faculty of MedicineTanta UniversityTanta 31511AlgahrbiaEgypt Department of Clinical Pathology Faculty of MedicineTanta UniversityTanta 31511AlgahrbiaEgypt Department of Pediatric University Medical CenterDr.Suliaman Al Habib Medical GroupManama 26671ManamaBahrain Department of Pediatric University Medical CenterKing Abdulla Medical CityArabian Gulf UniversityManama 26671ManamaBahrain

BACKGROUND Neonatal sepsis,a formidable threat to newborns,is a leading cause of neonatal mortality,with late-onset sepsis manifesting after 72 hours post-birth being particularly concerning.Pneumonia,a prevalent sepsis presentation,poses a significant risk,especially during the neonatal phase when lung defenses are compromised.Accurate diagnosis of pneumonia is imperative for timely and effective interventions.Saliva,a minimally invasive diagnostic medium,holds great promise for evaluating infections,especially in infants.AIM To investigate the potential of serum C-reactive protein(CRP),salivary CRP(sCRP),and mean platelet volume(MPV)as diagnostic markers for late-onset neonatal pneumonia(LONP).METHODS Eighty full-term neonates were systematically examined,considering anthropometric measurements,clinical manifestations,radiology findings,and essential biomarkers,including serum CRP,sCRP,and MPV.RESULTS The study reveals noteworthy distinctions in serum CRP levels,MPV,and the serum CRP/MPV ratio between neonates with LONP and healthy controls.MPV exhibited a robust discriminatory ability[area under the curve(AUC)=0.87]with high sensitivity and specificity at a cutoff value of>8.8.Correlations between serum CRP,sCRP,and MPV were also identified.Notably,sCRP demonstrated excellent predictive value for serum CRP levels(AUC=0.89),underscoring its potential as a diagnostic tool.CONCLUSION This study underscores the diagnostic promise of salivary and serum biomarkers,specifically MPV and CRP,in identifying and predicting LONP among neonates.These findings advocate for further research to validate their clinical utility in larger neonatal cohorts.

关键词： Neonatal sepsis Late-onset pneumonia Salivary C-reactive protein Mean platelet volume diagnostic markers Newborn infections

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MicroRNAs as diagnostic markers and therapeutic targets for traumatic brain injury

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Neural Regeneration Research 2017年第11期12卷 1749-1761页

作者： Bridget Martinez Philip V.Peplow Department of Molecular and Cellular Biology University of California Department of Anatomy University of Otago

Traumatic brain injury （TBI） is characterized by primary damage to the brain from the external mechanical force and by subsequent secondary injury due to various molecular and pathophysiological responses that eventually lead to neuronal cell death. Secondary brain injury events may occur minutes, hours, or even days after the trauma, and provide valuable therapeutic targets to prevent further neuronal degeneration. At the present time, there is no effective treatment for TBI due, in part, to the widespread impact of numerous complex secondary biochemical and pathophysiological events occurring at different time points following the initial injury. MicroRNAs control a range of physiological and pathological functions such as develop- ment, differentiation, apoptosis and metabolism, and may serve as potential targets for progress assessment and intervention against TBI to mitigate secondary damage to the brain. This has implications regarding improving the diagnostic accuracy of brain impairment and long-term outcomes as well as potential novel treatments. Recent human studies have identified specific microRNAs in serum/plasma （miR-425-p, -21, -93, -191 and -499） and cerebro-spinal fluid （CSF）（miR-328, -362-3p, -451, -486a） as possible indicators of the diagnosis, severity, and prognosis of TBI. Experimental animal studies have examined specific microRNAs as biomarkers and therapeutic targets for moderate and mild TBI （e.g., miR-21, miR-23b）. MicroRNA profil- ing was altered by voluntary exercise. Differences in basal microRNA expression in the brain of adult and aged animals and alterations in response to TBI （e.g., miR-21） have also been reported. Further large-scale studies with TBI patients are needed to provide more information on the changes in microRNA profiles in different age groups （children, adults, and elderly）.

关键词： traumatic brain injury microRNAs diagnostic markers therapeutic targets： humans animal models

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Screening of Postpartum Depression diagnostic markers Based on Immune-Related Genes and Immune Infiltration

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Psychosomatic Medicine Research 2022年第1期4卷 8-18页

作者： Yi-Dan Sun Xiao-Jiang Li Pei-Ying Yang Ying-Jie Jia First Teaching Hospital of Tianjin University of Traditional Chinese Medicine TianjinChina

Background:Postpartum depression(PPD)is a mild to severe non-psychotic depressive episode,one of the main factors leading to pregnancy-related morbidity and mortality,and a mental disorder that has not been fully diagnosed and treated.Compared with women without polycystic ovary syndrome,women with polycystic ovary syndrome are more likely to have a variety of pregnancy complications,including PPD.However,there is currently limited research on whether polycystic ovary syndrome is related to anxiety and depression during pregnancy,and whether this increases the risk of postpartum depression in women.Study design:The GSE10558 data set gene expression profile matrix was used for PPD expression profiles from Gene Expression Synthesis(GEO).The differentially expressed genes were selected and analyzed.Perform gene ontology(GO)enrichment and gene set variation analysis(GSVA)for annotation,visualization,and integrated discovery.At the same time,CIBERSORT and ESTIMATE were used to analyze the immune infiltration situation of the GSE10558 expression profile matrix,including the immune infiltration pattern of ovarian samples,and construct the immune cell infiltration(ICI)score.Then we screened the differentially expressed genes(DEGs)clustered with three groups of immune subtypes,and constructed a protein-protein interaction(PPI)and mRNA-miRNA-TF molecular interaction network.And further predicted the drug target of the hub gene and the target of small molecule compounds,and constructed a network.Based on the intersection of the phenotypic gene set,the pivot gene was identified.Finally,evaluate the expression differences of Hub genes between the data set groups,and generate receiver operating characteristic(ROC)curves to verify the diagnostic value of differentially expressed genes(DEG).Finally,genes with high area under the curve(AUC)values are validated.Results:We analyzed 222 DEGs with statistically significant differences in the GSE10558 data set by bioinformatics methods,of which 18 DEGs have significant differences.GO

关键词： Postpartum Depression diagnostic markers Immune-related genes Immune infiltration

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Cerebrospinal fluid diagnostic markers for two-dimensional electrophoresis-mass spectrometry in Parkinson’s disease patients

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Neural Regeneration Research 2010年第12期5卷 890-894页

作者： Ying Chen Gang Yu Wenbin Tu Hanchun Long Side Jiang Jincheng Wan Guoguang Peng Department of Neurology the First Affiliated Hospital. Chongqing Medical University Chongqing 400016 China

BACKGROUND： Previous studies have confirmed the existence of specific proteins in body fluid of Parkinson＇s disease （PD） patients. However, the existing research has contained several interference factors with poor reproducibility and has not focused on patients grouped according to disease duration. OBJECTIVE： To verify differential expression of proteins in cerebrospinal fluid of PD patients grouped in order of disease severity through the use of two-dimensional electrophoresis-mass spectrometry methods. DESIGN, TIME AND SETTING： The proteomic-based, case-control study was performed between September 2008 and June 2009 at the Key Laboratory of Neurology in the First Affiliated Hospital of Chongqing Medical University. PARTICIPANTS： A total of 52 outpatients and/or inpatients, who were admitted to the Department of Neurology in the First Affiliated Hospital of Chongqing Medical University between 2008 and 2009, were randomized into the present study. Among them, 27 PD patients served as the PD group and were assigned to three subgroups according to modified Webster, Hoehn, and Yahr rating scales： 14 = mild, 8 = moderate, and 5 = severe; non-PD group of 16 patients included 5 cases of viral meningitis, 3 cases of acute myelitis, 1 case of Guillain-Barre syndrome, 2 cases of tuberculous meningitis, 2 cases of restless legs syndrome, and 3 cases of essential tremor; control group （n = 9） consisted of muscular tension headache in 6 cases, as well as syncope, trigeminal neuralgia, idiopathic orthostatic hypotension in 1 case. METHODS： Cerebrospinal fluid was collected from the involved patients using the lumbar puncture method. Proteins in the cerebrospinal fluid were separated by two-dimensional electrophoresis. MAIN OUTCOME MEASURES： Characteristics of protein electrophoresis patterns were analyzed, differentially expressed proteins were detected using matrix-assisted laser desorption ionization time of flight mass spectrometry, and protein data were analyzed in the Mascot database. RESULTS： Five protein electrophe

关键词： cerebrospinal fluid two-dimensional gel electrophoresis mass spectrometry diagnostic markers proteomics Parkinson＇s disease neurodegenerative disease neural regeneration

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Thick tongue coating: diagnostic markers for metastatic colorectal cancer?

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TMR Cancer 2020年第4期3卷 144-152页

作者： Hao-Ran Zhang Xue Yang Cui-Hong Zhu Dan Chen Xiong-Zhi Wu Tianjin Hospital of Integrated Traditional Chinese and Western Medicine Tianjin 300100China Department of Medical Oncology Tianjin Medical University General HospitalTianjin 300052China Tianjin Cancer Hospital Airport Hospital Tianjin 300308China Tianjin Jingui Linglan Science and Technology Ltd.Tianjin 300000 China Tianjin Medical University Tianjin 300384China

Background:According to World Health Organization,colorectal cancer is the third most common cancer in the world.The prognosis assessment and condition judgment of the colorectal cancer remains a challenge clinically.Therefore,identification of diagnostic markers to evaluate the prognosis of colorectal cancer clinically should be urgently developed.We have observed that a lot of cancer patients had thick tongue clinically,but what is the relationship between tongue coating and the tumor?Methods:Seventy-four patients with colorectal carcinoma were collected through the outpatients of Tianjin Medical University Cancer Institute and Hospital from May 2010 to September 2011,in which there were 49 patients confirmed with recurrence or metastasis.All photos of patients’tongue were taken with a SONY camera in the same room and under constant conditions such as brightness or distance.Regression equation predicting thickness of tongue coating was constructed using binary logistic regression analysis.The optimal cut off of probabilities to diagnosis thick tongue coating was determined by receiver operating curve analysis.χ2 test for paired data and kappa test were used to determine the diagnostic value for recurrence and/or metastasis in colorectal cancer patients.Kolmogorov-Smirnov test was used to determine the distribution of ALT,AST,ALP ALB,TP,GLO,TBIL,DBIL,GGT,LDH,GLU,UA,CA724,CA199,CA242 and CEA.Data with the skewed distribution were presented as median(quartile interval).The association between the thickness of tongue coating and clinical-pathological character was evaluated by chi square test and two-independent-sample test.The two-independent-samplesχ2 was used to determine whether there were significant differences in the thin coating and thick coating between patients with recurrence and/or metastasis and patients without recurrence and/or metastasis.Kaplan-Meier method was used to analyze survival time.Statistical analysis was performed by SPSS(version 16.0).Results:Through retrospective clinical study we found

关键词： Colorectal cancer Tongue coating thickness Prognosis EGF lactic dehydrogenase diagnostic markers

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Fine-mapping and characterisation of genes on barley(Hordeum vulgare)chromosome 2H for salinity stress tolerance during germination

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The Crop Journal 2022年第3期10卷 754-766页

作者： Edward Mwando Yong Han Tefera Angessa Xiao-Qi Zhang Chengdao Li Western Crop Genetics Alliance College of ScienceHealthEngineering and EducationMurdoch UniversityMurdochWA 6150Australia Western Australian State Agricultural Biotechnology Centre Murdoch UniversityMurdochWA 6150Australia Department of Primary Industries and Regional Development 3 Baron-Hay CourtSouth PerthWA 6151Australia

Salinity causes a detrimental impact on plant growth,particularly when the stress occurs during germination and early development stages.Barley is one of the most salt-tolerant crops;previously we mapped two quantitative trait loci(QTL)for salinity tolerance during germination on the short arm of chromosome 2 H using a CM72/Gairdner doubled haploid(DH)population.Here,we narrowed down the major QTL to a region of 0.341 or 0.439 Mb containing 9 or 24 candidate genes belonging to 6 or 20 functional gene families according to barley reference genomes v1 and v3 respectively,using two DH populations of CM72/Gairdner and Skiff/CM72,F_(2)and F;generations of CM72/Gairdner/;Spartacus CL,Two Receptorlike kinase 4(RLPK4)v1 or Receptor-like kinase(RLK)v3 could be the candidates for enhanced germination under salinity stress because of their upregulated expression in salt-tolerant variety CM72.Besides,several insertion/deletion polymorphisms were identified within the 3 rd exon of the genes between CM72 and Gairdner.The sequence variations resulted in shifted functional protein domains,which may be associated with differences in salinity tolerance.Two molecular markers were designed for selecting the locus with receptor-like protein kinase 4,and one was inside HORVU2 Hr1 G111760.1 or HORVU.MOREX.r3.2 HG0202810.1.The diagnostic markers will allow for pyramiding of 2 H locus in barley varieties and facilitate genetic improvement for saline soils.Further,validation of the genes to elucidate the mechanisms involved in enhancing salinity tolerance at germination and designing RLPK4 specific markers is proposed.For this publication,all the analysis was based on barley reference genome of2017(v1),and it was used throughout for consistence.However,the positions of the markers and genes identified were updated according to new genome(v3)for reference.

关键词： Barley Germination Salinity tolerance diagnostic markers Receptor-like protein kinase 4 Gene expression Sequence analysis

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MMP14 is a diagnostic gene of intrahepatic cholangiocarcinoma associated with immune cell infiltration

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World Journal of Gastroenterology 2023年第19期29卷 2961-2978页

作者： Jun Wu Yang Guo Zhi-Fan Zuo Zi-Wei Zhu Lei Han China Medical University The General Hospital of Northern Theater Command Training Base for GraduateShenyang 110016Liaoning ProvinceChina Department of Hepatobiliary Surgery The General Hospital of Northern Theater CommandShenyang 110016Liaoning ProvinceChina Gynecological Radiotherapy Ward Liaoning Provincial Cancer HospitalShenyang 110801Liaoning provinceChina

BACKGROUND Intrahepatic cholangiocarcinoma(ICC)is a malignant tumor of the hepatobiliary system with concealed onset,strong invasiveness and poor prognosis.AIM To explore the disease characteristic genes that may be helpful in the diagnosis of ICC and affect immune cell infiltration.METHODS We downloaded two ICC-related human gene expression profiles from GEO database as the training group(GSE26566 and GSE32958 datasets)for difference analysis,and performed enrichment analysis on differential genes.The least absolute shrinkage and selection operator(LASSO),support vector machinerecursive feature elimination(SVM-RFE)and random forest(RF),three machine learning algorithms,were used to screen the characteristic genes.Double verification was carried out on GSE107943 and The Cancer Genome Atlas,two verification groups.Receiver operating characteristic curve and area under the curve(AUC)were used to evaluate the diagnostic efficacy of genes for ICC.CIBERSORT and ssGSEA algorithms were used to evaluate the effect of characteristic genes on immune infiltration pattern.Human Protein Atlas(HPA)was used to analyze the protein expression level of the target gene.RESULTS A total of 1091 differential genes were obtained in the training group.Enrichment analysis showed that the above genes were mainly enriched in small molecular catabolism,complement and coagulation cascade,bile secretion and other functions and pathways.Twentyfive characteristic genes were screened by LASSO regression,19 by SVM-RFE algorithm,and 30 by RF algorithm.Three algorithms were used in combination to determine the characteristic gene of ICC:MMP14.The verification group confirmed that the genes had a high diagnostic accuracy(AUC values of the training group and the verification group were 0.960,0.999,and 0.977,respectively).Comprehensive analysis of immune infiltration showed that MMP14 could affect the infiltration of monocytes,activated memory CD4 T cells,resting memory CD4 T cells,and other immune cells,and was closely related to the expression of CD

关键词： Intrahepatic cholangiocarcinoma MMP14 Machine learning Immune infiltration Characteristic gene diagnostic markers

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DNA methylation signatures in circulating cell-free DNA as biomarkers for the early detection of cancer

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Science China(Life Sciences) 2017年第4期60卷 356-362页

作者： Junyun Wang Xiao Han Yingli Sun Key Laboratory of Genomic and Precision Medicine China Gastrointestinal Cancer Research Center Beijing Institute of Genomics Chinese Academy of Sciences Beijing 100101 China University of Chinese Academy of Sciences Beijing 100049 China

Detecting cell-free DNA(cfDNA) or circulating tumor DNA(ctDNA) in plasma or serum could serve as a "liquid biopsy", which would be useful for numerous diagnostic applications. cfDNA methylation detection is one of the most promising approaches for cancer risk assessment. Here, we reviewed the literature related to the use of serum or plasma circulating cell-free DNA for cancer diagnosis in the early stage and their power as future biomarkers.

关键词： circulating cell-free DNA DNA methylation diagnostic markers cancer detection

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Clinical applications of exosome membrane proteins

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Precision Clinical Medicine 2020年第1期3卷 54-66页

作者： Qian Hu Hang Su Juan Li Christopher Lyon Wenfu Tang Meihua Wan Tony Ye Hu Center of Cellular and Molecular Diagnosis Tulane University School of MedicineNew OrleansLA 70112USA Department of Integrated Traditional Chinese and Western Medicine West China Hospital of Sichuan UniversityChengdu 610041Sichuan ProvinceChina Health Management Center West China Hospital of Sichuan UniversityChengdu 610041Sichuan ProvinceChina Department of Biochemistry&Molecular Biology Tulane University School of MedicineNew OrleansLA 70112USA

Extracellular vesicles(EVs)are small membranous particles that can mediate cell-to-cell communication and which are divided into at least three categories according to their subcellular origin and size:exosomes,microvesicles,and apoptotic bodies.Exosomes are the smallest(30–150 nm)of these EVs,and play an important role in EV-mediated cell-to-cell interactions,by transferring proteins,nucleic acids and,lipids from their parental cells to adjacent or distant cells to alter their phenotypes.Most exosome studies in the past two decades have focused on their nucleic acid composition and their transfer ofmRNAs and microRNAs to neighboring cells.However,exosomes also carry specific membrane proteins that can identify the physiological and pathological states of their parental cells or indicate their preferential target cells or tissues.Exosome membrane protein expression can also be directly employed or modified to allow exosomes to serve as drug delivery systems and therapeutic platforms,including in targeted therapy approaches.This review will briefly summarize information on exosome membrane proteins components and their role in exosome–cell interactions,including proteins associated with specific cell-interactions and diseases,and the potential for using exosome membrane proteins in therapeutic targeting approaches.

关键词： Exosome membrane protein endocytosis membrane fusion diagnostic markers exosome mimetics target therapy

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Research progress on O-GlcNAcylation in the occurrence,development,and treatment of colorectal cancer

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World Journal of Gastrointestinal Surgery 2021年第2期13卷 96-115页

作者： Yao Liu Fang-Xing Peng Department of Gastrointestinal Surgery The Second Affiliated Hospital of North Sichuan Medical CollegeMianyang 621000Sichuan ProvinceChina Department of Gastrointestinal Surgery Sichuan Mianyang 404 HospitalMianyang 621000Sichuan ProvinceChina

For a long time,colorectal cancer(CRC)has been ranked among the top cancerrelated mortality rates,threatening human health.As a significant posttranslational modification,O-GlcNAcylation plays an essential role in complex life activities.Related studies have found that the occurrence,development,and metastasis of CRC are all related to abnormal O-GlcNAcylation and participate in many critical biological processes,such as gene transcription,signal transduction,cell growth,and differentiation.Recently,nucleotide sugar analogs,tumorspecific carbohydrate vaccine,SIRT1 longevity gene,dendritic cells as targets,and NOTCH gene have become effective methods to induce antitumor therapy.Not long ago,checkpoint kinase 1 and checkpoint kinase 2 were used as therapeutic targets for CRC,but there are still many problems to be solved.With an in-depth study of protein chip,mass spectrometry,chromatography,and other technologies,O-GlcNAcylation research will accelerate rapidly,which may provide new ideas for the research and development of antitumor drugs and the discovery of new CRC diagnostic markers.

关键词： Colorectal cancer O-GlcNAcylation Antitumor therapy CHK1 and CHK2 Chip diagnostic markers

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