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An IB-LBM study of continuous cell sorting in deterministic lateral displacement arrays

Acta Mechanica Sinica 2016年第6期32卷 1023-1030页

作者： Qiang Wei Yuan-Qing Xu Xiao-Ying Tang Fang-Bao Tian School of Life Science Beijing Institute of Technology Key Laboratory of Convergence Medical Engineering System and Healthcare Technology The Ministry of Industry and Information Technology Beijing Institute of Technology School of Engineering and Information Technology University of New South Wales

The deterministic lateral displacement (DLD) is an important method used to sort particles and cells of different sizes. In this paper, the flexible cell sorting with the DLD method is studied by using a numerical model based on the immersed boundary-lattice Boltzmann method (IB-LBM). In this model, the fluid motion is solved by the LBM, and the cell membrane-fluid interaction is modeled with the LBM. The proposed model is validated by simulating the rigid particle sorted with the DLD method, and the results are found in good agreement with those measured in experiments. We first study the effect of flexibility on a single cell and multiple cells continuously going through a DLD device. It is found that the cell flexibility can significantly affect the cell path, which means the flexibility could have significant effects on the continuous cell sorting by the DLD method. The sorting characteristics of white blood cells and red blood cells are further studied by varying the spatial distribution of cylinder arrays and the initial cell-cell distance. The numerical results indicate that a well concentrated cell sorting can be obtained under a proper arrangement of cylinder arrays and a large enough initial cell-cell distance.

关键词： IB-LBM Cell sorting deterministic lateral displacement

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deterministic lateral displacement (DLD) based immunocapture microfluidics chip for simple and rapid detection of Cryptococcus neoformans in Cerebral spinal Fluid samples

Deterministic Lateral Displacement (DLD) based immunocapture...

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第十二届全国免疫学学术大会

作者： Xu Huan Daping hospital Third military medical university

Background/Objective:Cryptococcus neoformans infection can directly invade the central nervous system,causing Cryptococcus neoformans meningitis,which further results to severe illness,high mortality and *** rapid and accurate diagnosis of Cryptococcus neoformans is of great significance to the treatment of Cryptococcus neoformans *** current detection methods include smear ink stain detection,capsular antigen detection ***,Microfluidic chip as a miniaturization,high-throughput,integrated platform,has received significant attention in the early diagnosis of circulating tumor cells,pathogenic microbial ***,use of label free enrichment methods has achieved rapid and simple tumor cell and microbial separation based on physical characteristics including particle size,deformability,density and electric charge,and on the other hand,use of antigen antibody recognition also further improve the specificity and *** this study,we designed a label free immunocapture microfluidic chip for the diagnosis of Cryptococcus neoformans in cerebrospinal fluid according to the combination of label free deterministic lateral displacement(DLD) technique and immunological capture by anti-capsular antibody,finally,to provide a new method for the diagnosis of Cryptococcus neoformans ***:A microfluidic device made of an array triangular micropiUars,three inlet channels,and three outlet channels was designed based on deterministic lateral displacement principle using Auto-CAD *** microfluidic chip was fabricated following conventional AZ4620 photolithographic and PDMS casting methods,a clean glass as the *** neoformans were cultured using Sabouraud culture ***-capsular antibody was incubated on the triangular micropillars to capture the ***264.7 cells were used as the control *** efficiency and purity were ***:Cryptococcus neoformans can be efficiently capture

关键词： Cryptococcus neoformans deterministic lateral displacement immunocapture microfluidic chip

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