Deterministic Lateral Displacement (DLD) based immunocapture microfluidics chip for simple and rapid detection of Cryptococcus neoformans in Cerebral spinal Fluid samples
作者单位：Daping hospital Third military medical university
会议名称：《第十二届全国免疫学学术大会》
会议日期：2017年
学科分类：1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1010[医学-医学技术（可授医学、理学学位）] 100401[医学-流行病与卫生统计学] 10[医学]
关 键 词：Cryptococcus neoformans Deterministic Lateral Displacement immunocapture microfluidic chip
摘 要：Background/Objective:Cryptococcus neoformans infection can directly invade the central nervous system,causing Cryptococcus neoformans meningitis,which further results to severe illness,high mortality and *** rapid and accurate diagnosis of Cryptococcus neoformans is of great significance to the treatment of Cryptococcus neoformans *** current detection methods include smear ink stain detection,capsular antigen detection ***,Microfluidic chip as a miniaturization,high-throughput,integrated platform,has received significant attention in the early diagnosis of circulating tumor cells,pathogenic microbial ***,use of label free enrichment methods has achieved rapid and simple tumor cell and microbial separation based on physical characteristics including particle size,deformability,density and electric charge,and on the other hand,use of antigen antibody recognition also further improve the specificity and *** this study,we designed a label free immunocapture microfluidic chip for the diagnosis of Cryptococcus neoformans in cerebrospinal fluid according to the combination of label free Deterministic Lateral Displacement(DLD) technique and immunological capture by anti-capsular antibody,finally,to provide a new method for the diagnosis of Cryptococcus neoformans ***:A microfluidic device made of an array triangular micropiUars,three inlet channels,and three outlet channels was designed based on Deterministic Lateral Displacement principle using Auto-CAD *** microfluidic chip was fabricated following conventional AZ4620 photolithographic and PDMS casting methods,a clean glass as the *** neoformans were cultured using Sabouraud culture ***-capsular antibody was incubated on the triangular micropillars to capture the ***264.7 cells were used as the control *** efficiency and purity were ***:Cryptococcus neoformans can be efficiently capture