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dental mesenchymal stromal/stem cells in different microenvironments— implications in regenerative therapy

World Journal of stem cells 2021年第12期13卷 1863-1880页

作者： Ivana Okić-Đorđević Hristina Obradović Tamara Kukolj Anđelija Petrović Slavko Mojsilović Diana Bugarski Aleksandra Jauković Laboratory for Experimental Hematology and stem cells Institute for Medical ResearchUniversity of BelgradeBelgrade 11129Serbia

Current research data reveal microenvironment as a significant modifier of physical functions,pathologic changes,as well as the therapeutic effects of stem *** comparing regeneration potential of various stem cell types used for cytotherapy and tissue engineering,mesenchymal stem cells(MSCs)are currently the most attractive cell source for bone and tooth regeneration due to their differentiation and immunomodulatory potential and lack of ethical issues associated with their *** microenvironment of donors and recipients selected in cytotherapy plays a crucial role in regenerative potential of transplanted MSCs,indicating interactions of cells with their microenvironment indispensable in MSC-mediated bone and dental *** a variety of MSC populations have been procured from different parts of the tooth and tooth-supporting tissues,MSCs of dental origin and their achievements in capacity to reconstitute various dental tissues have gained attention of many research groups over the *** review discusses recent advances in comparative analyses of dental MSC regeneration potential with regards to their tissue origin and specific microenvironmental conditions,giving additional insight into the current clinical application of these cells.

关键词： Microenvironment dental mesenchymal stem cells Modulation of regenerative potential Tissue origin Hypoxia microenvironment Inflammatory microenvironment Clinical application

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Single CD271 marker isolates mesenchymal stem cells from human dental pulp

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International Journal of Oral Science 2015年第4期7卷 205-212页

作者： Ruth Alvarez Hye-Lim Lee Christine Hong Cun-Yu Wang Division of Oral Biology and Medicine School of DentistryUniversity of California at Los Angeles Section of Orthodontics School of DentistryUniversity of California at Los Angeles Department of Bioengineering Henry Samueli School of Engineering and Applied ScienceUniversity of California at Los Angeles

mesenchymal stem cells （MSCs） are a promising tool in regenerative medicine due to their capacity to differentiate into multiple lineages. In addition to MSCs isolated from bone marrow （BMSCs）, adult MSCs are isolated from craniofacial tissues including dental pulp tissues （DPs） using various stem cell surface markers. However, there has been a lack of consensus on a set of surface makers that are reproducibly effective at isolating putative multipotent dental mesenchymal stem cel^s （~M^Cs）. II1 ~his stucly, we used clif^et（~nt combinations of surface markers （CD51/CD140a, CD271, and STRO-1/CD146） to isolate homogeneous populations of DMSCs from heterogeneous dental pulp cells （DPCs） obtained from DP and compared their capacity to undergo multilineage differentiation. Fluorescence-activated cell sorting revealed that 27.3% of DPCs were CD51＋/CD140a＋, 10.6% were CD271＋, and 0.3% were STRO-1＋/CD146＋. Under odontogenic conditions, all three subsets of isolated DMSCs exhibited differentiation capacity into odontogenic lineages. Among these isolated subsets of DMSCs, CD271＋ DMSCs demonstrated the greatest odontogenic potential. While all three combinations of surface markers in this study successfully isolated DMSCs from DPCs, the single CD271 marker presents the most effective stem cell surface marker for identification of DMSCs with high odontogenic potential. Isolated CD271＋ DMSCs could potentially be utilized for future clinical applications in dentistry and regenerative medicine.

关键词： dental mesenchymal stem cells odontogenic differentiation cell surface markers dental pulp fluorescence-activated cellsorting

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Multidifferentiation potential of dental-derived stem cells

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World Journal of stem cells 2021年第5期13卷 342-365页

作者： Jing-Yao Yin Xing-Hong Luo Wei-Qing Feng Sheng-Hong Miao Ting-Ting Ning Qian Lei Tao Jiang Dan-Dan Ma Department of Stomatology Nanfang HospitalSouthern Medical UniversityGuangzhou 510515Guangdong ProvinceChina Department of Endodontics Stomatological HospitalSouthern Medical UniversityGuangzhou 510280Guangdong ProvinceChina

Tooth-related diseases and tooth loss are widespread and are a major public health *** loss of teeth can affect chewing,speech,appearance and even ***,the science of tooth regeneration has emerged,and attention has focused on tooth regeneration based on the principles of tooth development and stem cells combined with tissue engineering *** undifferentiated stem cells in normal tooth tissues,dental mesenchymal stem cells(DMSCs),which are a desirable source of autologous stem cells,play a significant role in tooth *** hope to reconstruct the complete tooth tissues with normal functions and vascularization by utilizing the odontogenic differentiation potential of ***,DMSCs also have the ability to differentiate towards cells of other tissue types due to their *** review focuses on the multipotential capacity of DMSCs to differentiate into various tissues,such as bone,cartilage,tendon,vessels,neural tissues,muscle-like tissues,hepatic-like tissues,eye tissues and glands and the influence of various regulatory factors,such as non-coding RNAs,signaling pathways,inflammation,aging and exosomes,on the odontogenic/osteogenic differentiation of DMSCs in tooth *** application of DMSCs in regenerative medicine and tissue engineering will be improved if the differentiation characteristics of DMSCs can be fully utilized,and the factors that regulate their differentiation can be well controlled.

关键词： dental mesenchymal stem cells Regenerative medicine Tissue engineering Multipotency Odontogenic differentiation Osteogenic differentiation

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Characterization of the osteogenic potential of mesenchymal stem cells from human periodontal ligament based on cell surface markers

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International Journal of Oral Science 2015年第4期7卷 213-219页

作者： Ruth Alvarez Hye-Lim Lee Cun-Yu Wang Christine Hong Division of Oral Biology and Medicine School of DentistryUniversity of California at Los Angeles Section of Orthodontics School of DentistryUniversity of California at Los Angeles

mesenchymal stem cell （MSC）-mediated therapy has been shown to be clinically effective in regenerating tissue defects. For improved regenerative therapy, it is critical to isolate homogenous populations of MSCs with high capacity to differentiate into appropriate tissues. The utilization of stem cell surface antigens provides a means to identify MSCs from various tissues. However, few surface markers that consistently isolate highly regenerative MSCs have been validated, making it challenging for routine clinical applications and making it all the more imperative to identify reliable surface markers. In this study, we used three surface marker combinations： CD51/CD140a, CD271, and STRO-1/CD146 for the isolation of homogenous populations of dental mesenchymal stem cells （DMSCs） from heterogeneous periodontal ligament cells （PDLCs）. Fluorescence-activated cell sorting analysis revealed that 24% of PDLCs were CD51＋/CD140a＋, 0.8% were CD271＋, and 2.4% were STRO-1＋/CD146＋. Sorted cell populations were further assessed for their multipotent properties by inducing osteogenic and chondrogenic differentiation. All three subsets of isolated DMSCs exhibited differentiation capacity into osteogenic and chondrogenic lineages but with varying degrees. CD271＋ DMSCs demonstrated the greatest osteogenic potential with strong induction of osteogenic markers such as DLX5, RUNX2, and BGLAP. Our study provides evidence that surface marker combinations used in this study are sufficient markers for the isolation of DMSCs from PDLCs. These results provide important insight into using specific surface markers for identifying homogenous populations of DMSCs for their improved utilization in regenerative medicine.

关键词： cell surface markers dental mesenchymal stem cells periodontal ligament

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