Single CD271 marker isolates mesenchymal stem cells from human dental pulp

作     者：Ruth Alvarez Hye-Lim Lee Christine Hong Cun-Yu Wang 

作者机构：Division of Oral Biology and MedicineSchool of DentistryUniversity of California at Los Angeles Section of OrthodonticsSchool of DentistryUniversity of California at Los Angeles Department of BioengineeringHenry Samueli School of Engineering and Applied ScienceUniversity of California at Los Angeles 

出 版 物：《International Journal of Oral Science》 (国际口腔科学杂志（英文版）)

年 卷 期：2015年第7卷第4期

页      面：205-212页

核心收录：

学科分类：1003[医学-口腔医学] 10[医学] 

基　　金：supported by National Institute of Dental and Craniofacial Research grant T90DE022734 

主　　题：dental mesenchymal stem cells odontogenic differentiation cell surface markers dental pulp fluorescence-activated cellsorting 

摘      要：Mesenchymal stem cells （MSCs） are a promising tool in regenerative medicine due to their capacity to differentiate into multiple lineages. In addition to MSCs isolated from bone marrow （BMSCs）, adult MSCs are isolated from craniofacial tissues including dental pulp tissues （DPs） using various stem cell surface markers. However, there has been a lack of consensus on a set of surface makers that are reproducibly effective at isolating putative multipotent dental mesenchymal stem cel^s （~M^Cs）. II1 ~his stucly, we used clif^et（~nt combinations of surface markers （CD51/CD140a, CD271, and STRO-1/CD146） to isolate homogeneous populations of DMSCs from heterogeneous dental pulp cells （DPCs） obtained from DP and compared their capacity to undergo multilineage differentiation. Fluorescence-activated cell sorting revealed that 27.3% of DPCs were CD51＋/CD140a＋, 10.6% were CD271＋, and 0.3% were STRO-1＋/CD146＋. Under odontogenic conditions, all three subsets of isolated DMSCs exhibited differentiation capacity into odontogenic lineages. Among these isolated subsets of DMSCs, CD271＋ DMSCs demonstrated the greatest odontogenic potential. While all three combinations of surface markers in this study successfully isolated DMSCs from DPCs, the single CD271 marker presents the most effective stem cell surface marker for identification of DMSCs with high odontogenic potential. Isolated CD271＋ DMSCs could potentially be utilized for future clinical applications in dentistry and regenerative medicine.