检索结果-南通市图书馆

2020年全国口腔颌面-头颈肿瘤学术大会暨中华口腔医学...

作者：刘潇李向军河北医科大学口腔医院

Purpose/Background:Xerostomia is a common disease caused in radiation therapy for oral cancer and systemic conditions such as Sjogren’s syndrome, etc. At present, there is no effective treatment for the treatment of the *** present study was designed to test the feasibility of inducing gland-differentiation from dental pulp cells by co-culturing them with cells derived from mandibular gland.

关键词： dental pulp cells differentiation Salivary glands Regenerative Fibroblasts salivary gland dysfunction diseases

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Influence of Adiponectin and Leptin on the Kinetics of Human pulp cells

引用

Open Journal of Stomatology 2020年第12期10卷 360-371页

作者： Isao Tamura Katsura Ueda Yoshifumi Matsuda Aiko Kamada Yoshihiro Yoshikawa Eisuke Domae Takashi Ikeo Shunji Kumabe Department of Oral Anatomy School of Dentistry Osaka Dental University 8-1 Kuzuhahanazono-cho Hirakata-shi Osaka Japan Department of Biochemistry School of Dentistry Osaka Dental University 8-1 Kuzuhahanazono-cho Hirakata-shi Osaka Japan

Adipocytokines, such as adiponectin and leptin, are expressed by adipocytes and are known as anti-metabolic syndrome factors. They are also thought to mediate calcification in mineralized tissue. We investigated the effects of adiponectin and leptin on the kinetics of human pulp cells using ELISA and western blot. After gaining informed consent, we obtained human pulp cells from three patients. After cultivation in Dulbecco’s Modified Eagle Medium (DMEM) without serum, cells from the 4th to 6th passages were incubated with various concentrations of adiponectin (1, 10, or 100 ng/mL) in DMEM or leptin (0.1, 1, or 10 ng/mL) in DMEM for 24 h. We confirmed that human pulp cells expressed adiponectin receptor 1 and leptin receptor. Although the proliferation index of these cells as measured by 5-bromo-2'-deoxyuridine (BrdU) incorporation increased in the presence of adiponectin in a dose-dependent manner, pulp cells stimulated with leptin showed no significant changes in BrdU incorporation. Alkaline phosphatase activity showed no significant changes after stimulation with either adipocytokine. Adiponectin induced expression of BMP-2 and osteopontin more strongly than did leptin. In particular, expression of BMP-2 increased in the presence of adiponectin in a dose-dependent manner. In contrast, the expression of dentin sialoprotein increased after stimulation with leptin. The expression of Runx2 was not observed from the cultured pulp cells stimulated with both molecules. These results indicate that adiponectin and leptin contribute to growth and differentiation of human pulp cells and may consequently affect the formation of secondary dentin or reparative dentin in the dentin-pulp complex.

关键词： dental pulp cells Adiponectin Leptin Bone Morphogenetic Protein-2 Dentin Sialoprotein Osteopontin ECM Formation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

A New Route for Preparation of β-TCP/ PLLA Composite

引用

Chinese Chemical Letters 2006年第6期17卷 833-836页

作者： Feng ZHANG Fang MEI Xin Zhi WANG Xiao Yang HU Yong Ming LUO Xu Liang DENG School and Hospital of Stomatology Peking University Beijing 100081 Department of Histology and Embryology Health Science Center Peking University Beijing 100083 Department of Environmental Science and Engineering Kunming University of Science and Technology Kunming 650093 The 2nd Hospital of Beijing Armed Police Forces Beijing 100037

A new facile route for preparation of β-TCP/PLLA composites is reported in this letter. SEM images display that β-TCP particles with average diameter of 400 nm were well bonded and distributed within the pore walls of the PLLA scaffolds. The mixture of the novel complex and human dental pulp cells was transplanted subcutaneously into the dorsal surface of a nude mouse. Two months later histological examination showed that new collagen and new dentin formed. The results revealed that the new nano β-TCP/PLLA composite combined with human pulp cells could induce dentin formation offering a new way to dental tissue engineering.

关键词： β-TCP particles β-TCP/PLLA composite dental pulp cells dental tissue engineering.

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Evaluating the potential of an amelogenin-derived peptide in tertiary dentin formation

引用

Regenerative Biomaterials 2021年第2期8卷 85-93页

作者： Xiu Peng Sili Han Kun Wang Longjiang Ding Zhenqi Liu Linglin Zhang State Key Laboratory of Oral Diseases Department of Cariology and EndodonticsNational Clinical Research Center for Oral DiseasesWest China Hospital of StomatologySichuan UniversityChengduChina

Several novel biomaterials have been developed for dental pulp capping by inducing tertiary dentin *** aim of this study was to evaluate the effect of QP5,an amelogenin-based peptide,on the mineralization of dental pulp cells(DPCs)in vitro and in *** cell viability of human DPCs(hDPCs)after treatment with QP5 was determined using the Cell Counting Kit-8(CCK-8).Migration of hDPCs was assessed using scratch assays,and the pro-mineralization effect was determined using alkaline phosphatase(ALP)staining,alizarin red staining and the expression of mineralization-related genes and *** results showed that QP5 had little effect on the cell viability,and significantly enhanced the migration capability of ***5 promoted the formation of mineralized nodules,and upregulated the activity of ALP,the expression of mRNA and proteins of mineralization-related genes.A pulp capping model in rats was generated to investigate the biological effect of *** results of micro-computed tomography and haematoxylin and eosin staining indicated that the formation of tertiary dentin in QP5-capping groups was more prominent than that in the negative control *** results indicated the potential of QP5 as a pulp therapy agent.

关键词： tertiary dentin amelogenin-derived peptide pulp capping dental pulp cells

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Insulin Effect on Gene Expression of dental pulp Cell during Osteodifferentiation

引用

Journal of Biosciences and Medicines 2022年第1期10卷 99-107页

作者： Fidan Huseynova Alamdar Mammadov Irada Huseynova Frédéric Cuisinier Véronique Barragan-Montero LBN University Montpellier Montpellier France Institute Molecular Biology and Biotechnology of ANAS Baku Azerbaijan

Insulin, an old but still actual molecule, has many roles in various cellular processes including bone formation and also angiogenesis. Insulin effects on different cell types were investigated, and we intended to check its effect on dental pulp cells (DPC) during osteoblast differentiation. First, bone differentiation ability of isolated dental pulp cells was assessed by alizarin red (AR) staining. Insulin increased dental pulp cell proliferation after 25 hours of culture. It increased mRNA expression of osteogenic markers such as Col1, RunX2, ALP, Osc, Mef2C and expression of genes involved in TGF b pathway such as Smad3, TSP1, VEGF at different time points.

关键词： dental pulp cells Gene Expression Osteodifferentiation Insulin

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：