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Phenolic Constituents with Antioxidative,Tyrosinase Inhibitory and Anti‑aging Activities from dendrobium loddigesii Rolfe

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Natural Products and Bioprospecting 2019年第5期9卷 329-336页

作者： Rui-Jing Ma Liu Yang Xue Bai Jin-Yu Li Ming-Yan Yuan Ya-Qin Wang Yong Xie Jiang-Miao Hu Jun Zhou State Key Laboratory of Phytochemistry and Plant Resources in West China Kunming Institute of BotanyChinese Academy of SciencesKunming 650201People’s Republic of China College of Pharmacy Guilin Medical UniversityGuilin 541004People’s Republic of China R&D Center of Dr.Plant Kunming Institute of BotanyChinese Academy of SciencesKunming 650201People’s Republic of China

Aqueous ethanol extracts of powdered stems of dendrobium loddigesii afforded three new phenolics including threo-7-Oethyl-9-O-(4-hydroxyphenyl)propionyl-guaiacylglycerol(1),(R)-4,5,4ʹ-trihydroxy-3,3ʹ,α-trimethoxybibenzyl(2)and(S)-5,5′,7-trihydroxy-3′,4′-dimethoxyflavanone(3),together with eleven known *** structures were determined by extensive spectroscopic *** identify natural antioxidants,whitening,and anti-aging agents,the abilities of these phenolics were assessed to scavenge the 1,2-diphenyl-2-picrylhydrazyl(DPPH)radical,their abilities to inhibit tyrosinase production,and their abilities to stimulate collagen production by human dermal fibroblasts-adult(HDFa)*** was found that compounds 1,4-8,13 and 14 exhibited significant DPPH radical scavenging activities,compound 10 exhibited tyrosinase inhibitory activity(IC_(50)37.904μg/mL),and compound 9 showed significant collagen production with an EC_(50)value of 3.182μg/*** results suggest that phenolic constituents from *** may be candidate antioxidants,skin-whitening and/or anti-aging agents.

关键词： dendrobium loddigesii Phenolic constituents Antioxidative Tyrosinase inhibitory Anti-aging

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Comparison of Stomatal Characteristics between dendrobium loddigesii and dendrobium candidum

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Agricultural Science & Technology 2011年第8期12卷 1161-1165页

作者：孙志蓉王美云张宏桂金家兴李碧彪北京中医药大学中药学院北京100102 河北省廊坊市人民医院河北廊坊065000 贵州吉仁堂药业公司贵州兴义562400

[Objective]This study was to reveal the physiological and ecological mechanism of growth and development of test-tube seedlings.[Methods]Leaf stomata of test-tube seedlings of *** and *** at different growth and development stages were observed under scanning electron microscope,and their fluorescence induction response curve was assayed by using modulate fluorometer.[Results] At each growth and development stage,*** test-tube seedling has a higher leaf stomatal density over ***,while a lower stomata opening *** with the growth,the size of stomata of both *** and *** did not change obviously,but their stoma density increased by 83% and 17.6% *** stomata opening rate reached the highest at the age of *** the conditions of three settled light intensities,stomatal opening degree of *** was increased by the intensity of illumination,the maximum aperture occurred at the light intensity of 54 μmol/m2·*** the same culture condition,both the maximum electron transport rate（14 μmol/m2·s）and light saturation point（318 μmol/m2·s）of *** test-tube seedling are lower than that of ***（20 μmol/m2·s,483 μmol/m2·s）.Moreover,the potential photosynthetic capacity of *** is relatively poor.[Conclusion]The photosynthetic rate varies among different species of dendrobium,which could be attributed to their different stomatal ***,the light intensity of tissue culture chamber is set up as 27 μmol/（m2·s）,where both *** and *** do not acquire an optimal state for their leaf stomatal *** the characteristics to select suitable culture condition,the stomatal aperture and photosynthetic rate could be enhanced,which can promote the growth and development of test-tube seedlings.

关键词： dendrobium loddigesii dendrobium candidum Tissue culture Stomata Photosynthetic characteristics

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