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Ratiometric fluorescence detection of bleomycin based on proximity-dependent fluorescence conversion of dna-templated silver nanoclusters

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Chinese Chemical Letters 2021年第2期32卷 906-909页

作者： Lingyuan Liu Shuyun Zhu Jing Sun Meng Xia Xian’en Zhao Guobao Xu College of Chemistry and Chemical Engineering Qufu Normal UniversityQufu 273165China Qinghai Key Laboratory of Qinghai-Tibet Plateau Biological Resources Northwest Institute of Plateau BiologyChinese Academy of SciencesXining 810001China State Key Laboratory of Electroanalytical Chemistry Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchun 130022China

We design a ratiometric fluo rescent sensing platform for bleomycin(BLM) by using proximity-dependent dna-templated silver nanoclusters(dna-AgNCs) *** ratiometric sensing system is constructed with dna-AgNCs as single *** proposed strategy is based on the two following facts:(1) a covert dna can approach and transform the dna-AgNCs with green emission(G-dna-AgNCs) into red emission through hybridization reaction.(2) The specific cleavage of the convert dna by BLM in the presence of Fe(Ⅱ) inhibits the discoloration of ***,benefiting from the specific recognition of BLM and unique properties of G-dna-AgNCs,a hignly-sensitive ratiometric sensor for BLM has been successfully *** detection limit is as low as 30 pmol/*** label-free fluorescence probe possesses advantages of convenient synthetic process and low ***,this ratiometric method has been applied to the detection of BLM in human serum samples,illustrating a promising tool for analysis of BLM in cancer therapy.

关键词： Ratiometric nanosensor dna-templated silver nanoclusters Fluorescence transformation Bleomycin dna scission

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dna-templated silver nanoclusters light up tryptophan for combined detection of plasma tryptophan and albumin in sepsis

DNA-templated silver nanoclusters light up tryptophan for co...

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第十四届全国免疫学学术大会

作者： Jingpu Zhang Lei Pan Tie Fu Liu Shanghai Public Health Clinical Center

Tryptophan(Trp) as an essential amino acid plays critical roles in regulating multiple cell activities,and the changes of its circulating level usually indicate disease status such as the severity of acute inflammation ***,the current technology for Trp detection mostly relies on chromatography that cannot meet the rapid and simple detection requirement in clinical ***,a label-free fluorescent nanosensor was constructed to detect Trp and its carrier proteinhuman serum albumin(HSA) in *** nanosensor consisted of a cytosine(C)-rich signal probe of dna-templated silver nanocluster(AgNCs/dna) and a Trp(Trp) aptamer-based capture probe with a guanine(G)-rich *** UV irradiation,Trp was found to trigger G-rich sequence-mediated fluorescence enhancement effect for AgNCs/*** on this unique property,the nanosensor exhibited the limit of detection of 0.43 μM for Trp,superior to the current fluorescence-based detection method,and it gave specific photochemical response to indole *** applying in plasma detection,the nanosensor resisted physiological level of NaCl in plasma,but be quenched by trace volume of HSA,which facilitates the combined HSA detection with only 1 μL plasma ***,total Trp detection based on the nanosensor in deproteinized plasma samples successfully distinguished sepsis from normal *** simple procedure of "mix,exposure and detection" together with its ultralow sampling volume,time-saving and cost-effective,sensitive and selective properties offers the nanosensor great potential for future sepsis diagnosis and classification.

关键词： dna-templated silver nanoclusters photochemical reaction aptamer tryptophan albumin

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