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Identification of Andrographis Herba and its common products using mini-barcode

Chinese Journal of Natural Medicines 2022年第5期20卷 393-400页

作者： XIONG Chao HUANG Chen-Hao WU Lan XU Ran XUE Jian-Ping LIU Zhi-Guo SUN Wei School of Life Science and Technology Wuhan Polytechnic UniversityWuhan 430023China Institute of Chinese Materia Medica China Academy of Chinese Medical SciencesBeijing 100700China College of Life Sciences Huaibei Normal UniversityHuaibei 235000China

Andrographis Herba,the aerial part of Andrographis paniculata(Burm.f.)Wall.ex Nees(Acanthaceae),has a wide geographic distribution and has been used for the treatment of fever,cold,inflammation,and other infectious diseases.In markets,sellers and buyers commonly inadvertently confuse with related species.In addition,most Chinese medicinal herbs are subjected to traditional processing procedures,such as steaming and boiling,before they are sold at dispensaries;therefore,it is very difficult to identify Andrographis Herba when it is processed into Chinese medicines.The identification of species and processed medicinal materials is a growing issue in the marketplace.However,conventional methods of identification have limitations,while dna barcoding has received considerable attention as a new potential means to identify species and processed medicinal materials.In this study,17 standard reference materials of A.paniculata,2 standard decoctions,27 commercial products and two adulterants were collected.Based on the ITS2 sequence,it could successfully identify A.paniculata and adulterants.Moreover,a nucleotide signature consisting of 71 bp was designed,this sequence is highly conserved and specific within A.paniculata while divergent among other species.Then,we used these new primers to amplify the nucleotide signature region from processed materials.In conclusion,the dna barcoding method developed in the present study for authenticating A.paniculata is rapid and cost-effective.It can be used in the future to guarantee the quality of Andrographis Herba of each regulatory link for clinical use.

关键词： dna barcoding Andrographis Herba Andrographis paniculata Molecular identification

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26S rdna D1-D3 Region:A Novel dna barcoding for Amomum villosum Lour.

26S rDNA D1-D3 Region:A Novel DNA Barcoding for Amomum villo...

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多学科交叉，产学研联合，实现西部药用植物资源的可持续利...

作者： Huang Qionglin~1,Yang Jinfen~1,Chen Hongyan~2,Zhan Ruoting~1,Ma Xinye~1,Xu Hui~(1,*), Chen Weiwen~(1*) 1 Research Center of Chinese Herbal Medicinal Science and Engineering,Guangzhou University of Chinese Medicine,Key Laboratory of Chinese Medicinal Resources of Ministry of Education,Guangzhou 510006 2College of Chinese Medicine,Guangzhou University of Chinese Medicine,Guangzhou 510006

In order to screen out appropriate dna barcoding for Amomum villosum Lour.and establish molecular identification method of three original plants of Sharen Fructus Amomi,we analyzed four candidate fragment(ITS2,ITS1-5.8S-ITS2,psbA-trnH,26S rdna D1-D3)of Amomun villosum Lour.from different produce area,Amomum villosum Lour.***.xanthioides T.L.Wu et Senjen and Amomum longiligulare T.L.Wu,and constructed phylogenetic trees based on the sequences.we

关键词： 26S rdna D1-D3 Amomum villosum Lour dna barcoding

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Molecular identification of the potentially forensically relevant cluster flies Pollenia rudis (Fabricius) and Pollenia vagabunda (Meigen) (Diptera: Polleniidae) — non-recorded species in Algeria

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Forensic Sciences Research 2022年第1期7卷 69-77页

作者： Meriem Taleb Ghania Tail Halide Nihal Açıkgöz Department of Population and Organism Biology University of Blida 1BlidaAlgeria Department of Forensic Biology Ankara UniversityMamakAnkaraTurkey

Cluster flies are represented by the genus Pollenia Robineau-Desvoidy,1830 of the family Polleniidae Brauer and Bergenstamm,1889.Their larvae are known to be internal parasites or predators of earthworms.Herein,we report for the first time the occurrence of the cluster flies Pollenia rudis Fabricius,1794 and Pollenia vagabunda(Meigen,1826)(Diptera:Polleniidae)on carcasses in Algeria and identify them through dna barcoding.A region of the mitochondrial cytochrome c oxidase I gene(COI)was amplified and sequenced.Genetic distances were determined.A phylogenetic tree was constructed with the maximum parsimony method using 10000 bootstrap replicates.A total number of 157 adults of P.rudis were collected together with 325 adults of Pollenia vagabunda.The occurrence of Pollenia on animal carcasses does not seem to be correlated with a particular stage of decomposition.All the sequences were correctly identified using the BLASTn tool from the GenBank database and the BOLD identification engine.Intra-and interspecific sequence divergence values were less than 1%and greater than 3%,respectively.COI barcodes obtained from this study were robust enough to identify and distinguish unambiguously between P.rudis and P.vagabunda.In the tree-based analysis,the cluster flies were all assigned to their respective species separately from each other confirming the morphological identification.These results provide dna barcodes that contribute to the growth of reference databases and allow fast and accurate identification.

关键词： Forensic sciences forensic entomology Pollenia cytochrome c oxidase I dna barcoding North Africa

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Molecular authentication of the fashionable dainty Eriocheir japonica sinensis based on mitochondrial dna barcoding

Molecular authentication of the fashionable dainty Eriocheir...

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中国甲壳动物学会第十一届年会暨学术研讨会

作者： Daizhen Zhang,Boping Tang,Huabin Zhang,Ge Ding Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Yancheng Normal University,Xiwang Road,224051 Yancheng,China

＜正＞Eriocheirjaponica sinensis has long been regarded as a fashionable dainty in China and other Asian countries for its good taste and rich nutrition.The largest and most economically important mitten crab belongs to E.j.sinensis.But now,E.j.sinensis resource has been intermixed with E.j.hepuensis,which is similar to E.j.sinensis in morphology.In addition,the germplasm resource will be declining rapidly for

关键词： AS-PCR SNP dna barcoding Molecular authentication Eriocheir japonica sinensis

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Differentiation of Dendrobium by potential dna barcoding sequences

Differentiation of Dendrobium by potential DNA barcoding seq...

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中华中医药学会第九届中药鉴定学术会议

作者： Hui Yao,Jingyuan Song,Xinye Ma,Ying Li,Shilin Chen*,Jianping Han,Linchun Shi,Xiwen Li (The Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences, Beijing 100193,PR China)

dna barcoding facilitate species identification and recognition using standard dna sequence.The primary challenge for barcoding plants has been to identify a suitable region on which to focus the effort.The genus Dendrobium includes 74 species and 2 varieties and more than half of them are the origin of Herba Dendrobii,which makes the authentication of the origin of Herba Dendrobii difficult for consumers and provide a challenging test for barcoding plants.In current study,the sequences of matK,rbcL.accD,ndhJ,rpoCl,rpoB,YCF5 and trnH-psbA intergenic spacer of 30 medicinal Dendrobium species and 1 species of Bulbophyllum odoratissimum were sequenced and analyzed to barcode the genus Dendrobium.The results indicated that six of the regions(rbcL,accD,ndhJ,rpoCl,rpoB,YCF5) were conserved and two of the regions(matK and trnH-psbA) showed promise for barcoding the genus Dendrobium.

关键词： dna barcoding Dendrobium Orchidaceae identification

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Molecular Identification of Corni Fructus Using dna barcoding

Molecular Identification of Corni Fructus Using DNA Barcodin...

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第十五届中国科协年会第21分会场：中药与天然药物现代研...

作者： HOU Dianyun SONG Jingyuan YAO Hui HAN Jianping PANG Xiaohui SHI Linchun YANG Pei WANG Xiaochen CHEN Shilin Institute of Medicinal Plant Development Henan University of Science and Technology

Genomic dna were extracted from Corni Fructus and its adulterants. The ITS (internal trascribed spacer) regions were amplified using polymerase chain reaction. Sequence assembly was performed using CodonCode Aligner V 3.5.4. Genetic distances were computed using MEGA V 5.0. Species identification was conducted using neighbor-joining (NJ) trees. The results showed that the ITS sequence length of Corni Fructus was 659 bp. The average intra-specific genetic distance of Corni Fructus was 0.005, markedly lower than the inter-specific genetic distance between Corni Fructus and its adulterants (0.357). NJ trees results indicated that Corni Fructus and its adulterants can be easily differentiated with monophyly. ITS regions can accurately and efficiently distinguish Corni Fructus and its adulterants. In addition, the results not only established the foundation for the clinical safety in the utilization of Corni Fructus, but also provided reference for molecular identification of other Chinese herbal medicine and Chinese herbal pieces.

关键词： Corni Fructus dna barcoding molecular identification

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Comparative performance of the 16S rRNA,12S rRNA and COI genes in dna barcoding of anurans

Comparative performance of the 16S rRNA,12S rRNA and COI gen...

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浙江省动物学会第十二届会员代表大会暨学术讨论会

作者： Yunjie Pan Sidan Tao Wei Wang Tingting wang Jiayong Zhang College of Chemistry and Life Science Zhejiang Normal University

The 5’ region of the mitochondrial dna(mtdna) gene cytochrome c oxidaseⅠ(COⅠ) is the first-choice marker for dna barcoding.However,because COⅠtends to be highly variable in amphibians,sequencing is often challenging.Consequently,other comparatively conserved mtdna genes,16S rRNA gene and 12S rRNA gene,are often advocated for amphibian barcoding. Herein,we directly compare the usefulness of COⅠ,16S and 12S in discriminating species of common economic frogs and the possiblity to identify species and discover cryptic species in resource protection using 327 individuals(Anura:7 Family,19 Genus,35 Species).Analysis of Kimura 2-parameter genetic distances(K2P) documents the mean intraspecific variation for COⅠ,16S rRNA and 12S rRNA genes to be 6.0%,0.8%and 1.2%;the mean interspecific variation to be 13.5%,9.7%and 9.4%;Intra- and interspecific genetic divergences occasionally overlap in all the three markers,thus reducing the value of a barcoding gap to identify genera.Except Pelophylax,Vibrissaphora and Bufo,all the three dna barcoding markers can identify species.In comparion of COⅠ,16S and 12S rRNA genes,COⅠis the best dna barcoding marker for frogs, and 12S rRNA gene is better than 16S rRNA gene.Genetic distances for COⅠ,16S and 12S rRNA genes of of P.nigromaculata,P.plancyi,P.terentievi in Pelophylax are far closer than the threshold value,implying species hybridization may exist.However,P.pleuraden have a significant genetic differences with other species of Pelophylax.Analysis of NJ phylogenetic tree documents P.pleuraden and Babina adenopleura forming a sister group relationship,showing P. pleuraden should be moved form Pelophylax to Babina,named as Babina pleuraden.Whereas genetic distances for COⅠ,16S and 12S Hoplobatrachus rugulosus,Frvarya limnoejecharis and Odorrana schmackeri are further than the threshold value to distinguish species,which implies the possibility of the existence cryptic species.Based on COⅠ,16S,12S rRNA genes,we analyse all samples of F.limnocharis based on Network,which all prove the ex

关键词： dna barcoding COⅠgene 16SrRNAgene 12SrRNAgene Genetic distance Cryptic species

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铁皮石斛遗传多样性、中国石斛属的系统发育dna barcoding研究

铁皮石斛遗传多样性、中国石斛属的系统发育DNA Barcoding研究

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第八届全国药用植物及植物药学术研讨会

作者：丁小余丁鸽李雪霞南京师范大学生命科学学院

铁皮石斛是中国特有的珍稀濒危多年生兰科植物,在我国南方曾有着广泛的分布,但现在该物种的数量已锐减,被载入《中国植物红皮书》。本研究采用AFLP分子标记的方法,对铁皮石斛12个野生居群进行了遗传多样性以及居群结构进行了探讨。结果... 详细信息

铁皮石斛是中国特有的珍稀濒危多年生兰科植物,在我国南方曾有着广泛的分布,但现在该物种的数量已锐减,被载入《中国植物红皮书》。本研究采用AFLP分子标记的方法,对铁皮石斛12个野生居群进行了遗传多样性以及居群结构进行了探讨。结果表明:POPGENE揭示了该物种具有较高的遗传多样性水平(H=0.269),AMOVA分析了居群之间的分化指数,φ值从0.047到0.578,平均有26.97%

关键词：铁皮石斛 AFLP 遗传多样性分子系统发育 dna barcoding

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Identification of Chinese commercial "Lingzhi"(Ganoderma) using dna barcoding based on ITS2 Loci

Identification of Chinese commercial "Lingzhi"(Ganoderma) us...

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2013全国中药与天然药物高峰论坛暨第十三届全国中药和...

作者： Baosheng Liao Xiaochen Chen Jingyuan Song Shilin Chen Jianping Han Institute of Medicinal Plant Development Chinese Academy of Medical Sciences and Peking Union Medical College

Since recorded in Shengnong’s Compendium of Material Medica,"Lingzhi" has been medicinally applied more than 2,000 years in China.Currently,"Chi-zhi" and "Zi-zhi" are the widely used species.There is a variety of pro... 详细信息

关键词： Ganoderma lucidum Ganoderma sinense identification dna barcoding

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Applying dna barcoding Technique to Identify Traditional SHE Medicine Shi-Liang-Cha and Some Chimonanthus Species

Applying DNA Barcoding Technique to Identify Traditional SHE...

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第十四届全国中药和天然药物学术研讨会

作者： Kejun Cheng Shuangjiao Ma Jinbiao Xu Qundan Lv Hui Yao Shilin Chen Lishui Institute of Agriculture Sciences Fudan University Chinese Academy of Medical Sciences & Peking Union Medical College China Academy of Chinese Medical Sciences

Traditional SHE Medicine is an important part of folk medicine with a long history and has attracted high attention of ethnic Han and SHE minorities in the southeast of China,especially in Zhejiang and Fujian Provinces.Shi-Liang-Cha,one of most widely used SHE medicines,was made from leaves of two species in genus Chimonanthus,C.salicifolius Hu and C.zhejiangensis M.C.Liu.The known studies revealed chemical differences and biodiversities between Shi-Liang-Cha and some other Chimonanthus species,such as C.nitens Oliv.,C.praecox（L.） Link.The taxonomy,origin and evolution of Chimonanthus have been controversial and sometimes authentication of the species is not easy by means of traditional morphological features.dna barcoding has become one of hotspots of species identification and biodiversity research in recent years.It is a new biological method using short and universal segments of dna,with which species identification is performed rapidly and accurately.In this study,dna barcoding technology was used to identify Shi-Liang-Cha（C.salicifolius Hu and C.zhejiangensis M.C.Liu.） and some other Chimonanthus species based on internal transcribed spacer 2（ITS2） in order to ensure the accuracy and safety of Chimonanthus during the clinical practice.A further study to identify other SHE medicines will be carried out on the basis of the successful identification of Chimonanthus and our research would provide technical support for the development and research of Traditional SHE Medicine,and make a new step on its application,especially in the field of quality controls.

关键词： Chimonanthus ITS2 Identification SHE medicine dna barcoding

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