Molecular identification of the potentially forensically relevant cluster flies Pollenia rudis (Fabricius) and Pollenia vagabunda (Meigen) (Diptera: Polleniidae) — non-recorded species in Algeria

作     者：Meriem Taleb Ghania Tail Halide Nihal Açıkgöz Meriem Taleb;Ghania Tail;Halide Nihal A?ikg?z

作者机构：Department of Population and Organism BiologyUniversity of Blida 1BlidaAlgeria Department of Forensic BiologyAnkara UniversityMamakAnkaraTurkey 

出 版 物：《Forensic Sciences Research》 (法庭科学研究（英文）)

年 卷 期：2022年第7卷第1期

页      面：69-77页

核心收录：

学科分类：0710[理学-生物学] 1001[医学-基础医学(可授医学、理学学位)] 07[理学] 08[工学] 0838[工学-公安技术] 100105[医学-法医学] 071002[理学-动物学] 10[医学] 

基　　金：This study was approved by the Institutional Review Board of University of Blida 1(USDB1-SNV) 

主　　题：Forensic sciences forensic entomology Pollenia cytochrome c oxidase I DNA barcoding North Africa 

摘      要：Cluster flies are represented by the genus Pollenia Robineau-Desvoidy,1830 of the family Polleniidae Brauer and Bergenstamm,*** larvae are known to be internal parasites or predators of ***,we report for the first time the occurrence of the cluster flies Pollenia rudis Fabricius,1794 and Pollenia vagabunda(Meigen,1826)(Diptera:Polleniidae)on carcasses in Algeria and identify them through DNA barcoding.A region of the mitochondrial cytochrome c oxidase I gene(COI)was amplified and *** distances were determined.A phylogenetic tree was constructed with the maximum parsimony method using 10000 bootstrap replicates.A total number of 157 adults of *** were collected together with 325 adults of Pollenia *** occurrence of Pollenia on animal carcasses does not seem to be correlated with a particular stage of *** the sequences were correctly identified using the BLASTn tool from the GenBank database and the BOLD identification ***-and interspecific sequence divergence values were less than 1%and greater than 3%,*** barcodes obtained from this study were robust enough to identify and distinguish unambiguously between *** and *** the tree-based analysis,the cluster flies were all assigned to their respective species separately from each other confirming the morphological *** results provide DNA barcodes that contribute to the growth of reference databases and allow fast and accurate identification.