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Biotic and abiotic stress-responsive genes are stimulated to resist drought stress in purple wheat

Journal of Integrative Agriculture 2020年第1期19卷 33-50页

作者： LI Xiao-lan LÜXiang WANG Xiao-hong PENG Qin ZHANG Ming-sheng REN Ming-jian School of Life Sciences Guizhou University/State Engineering Technology Institute for Karst Desertification Control/Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous RegionMinistry of EducationGuiyang 550025P.R.China School of Agriculture Guizhou University/Guizhou Sub-Center of National Wheat Improvement CenterGuiyang 550025P.R.China Special Key Laboratory of Microbial Resources and Drug Development from Higher Education Institution of Guizhou Province/Research Center for Medicine and Biology Zunyi Medical UniversityZunyi 563006P.R.China

Triticum aestivum L. cv. Guizi 1(GZ1) is a drought-tolerant local purple wheat cultivar. It is not clear how purple wheat resists drought stress, but it could be related to anthocyanin biosynthesis. In this study, transcriptome data from droughttreated samples and controls were compared. Drought slightly reduced the anthocyanin, protein and starch contents of GZ1 grains and significantly reduced the grain weight. Under drought stress, 16 682 transcripts were reduced, 27 766 differentially expressed genes(DEGs) were identified, and 379 DEGs, including DREBs, were related to defense response. The defense-response genes included response to water deprivation, reactive oxygen, bacteria, fungi, etc. Most of the structural and regulatory genes in anthocyanin biosynthesis were downregulated, with only Ta DFR, Ta OMT, Ta5,3GT, and Ta MYB-4 B1 being upregulated. Ta CHS, Ta F3H, TaCHI, Ta4CL, and TaF3’H are involved in responses to UV, hormones, and stimulus. Ta CHS-2D1, Ta DFR-2D2, Ta DFR-7D, TaOMT-5A, Ta5,3 GT-1B1, Ta5,3GT-3A, and Ta5,3GT-7B1 connect anthocyanin biosynthesis with other pathways, and their interacting proteins are involved in primary metabolism, genetic regulation, growth and development, and defense responses. There is further speculation about the defense-responsive network in purple wheat. The results indicated that biotic and abiotic stress-responsive genes were stimulated to resist drought stress in purple wheat GZ1, and anthocyanin biosynthesis also participated in the drought defense response through several structural genes.

关键词： transcriptome purple wheat drought anthocyanin differentially expressed genes defense response stress

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Molecular characterization of a defense-related AMP-binding protein gene, OsBIABP1, from rice

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2009年第10期10卷 731-739页

作者： Xin-chun ZHANG Xin YU Hui-juan ZHANG Feng-ming SONG State Key Laboratory for Rice Biology Institute of Biotechnology Zhejiang University Hangzhou 310029 China

We cloned and characterized a rice gene OsBIABPI encoding an AMP-binding protein. The full-length cDNA of OsBIABP1 is 1912-bp long and is predicted to encode a 558-aa protein. OsBIABP1 contains a typical AMP-binding signature motif and shows high similarity to members of AMP-binding protein family. OsBIABP1 is expressed in stems, leaves and flowers of rice plants, but is not expressed, or expressed at a very low level, in rice roots. The expression of OsBIABP1 was induced by some defense-related signal molecules, e.g., salicylic acid （SA）, benzothiadiazole, jasmonic acid （JA）, and 1-amino cyclopropane- 1-carboxylic acid, which mediate SA- and JA/ethylene （ET）-dependent defense signaling pathways, respectively. Furthermore, the expression of OsBIABP1 is activated by the infection ofMagnaporthe oryzae, and the induced expression is quicker and stronger during early stages ofpathogenesis in incompatible interaction than that in compatible interaction between rice and M. oryzae. Our results suggest that OsBIABP1 may be a defense-related AMP-binding protein that is involved in the regulation of defense response through SA and/or JA/ET signaling pathways.

关键词： Rice Oryza sativa L. Magnaporthe oryzae defense response OsBIABP1 AMP-binding protein

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Comparative transcriptome analysis reveals key genes and pathways in response to Alternaria alternata apple pathotype infection

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Horticultural Plant Journal 2024年第3期10卷 641-656页

作者： Kai Liu Zhaolin Liang An Yang Jiadi Yan Peihua Cong Xiaolei Han Caixia Zhang Key Laboratory of Horticultural Crop Germplasm Utilization of the Ministry of Agriculture and Rural Affairs(MOA) Research Institute of PomologyChinese Academy of Agricultural SciencesXingchengLiaoning 125100China

Apple leaf spot,caused by the Alternaria alternata apple pathotype(AAAP),is an important fungal disease of *** understand the molecular basis of resistance and pathogenesis in apple leaf spot,the transcriptomes of two apple cultivars‘Hanfu'(HF)(resistant)and‘Golden Delicious'(GD)(susceptible)were analyzed at 0,6,18,24 and 48 h after AAAP inoculation by *** each time point,a large number of significantly differentially expressed genes(DEGs)were screened between AAAP-inoculated and uninoculated apple *** of the common DEGs at four time points revealed significant differences in the resistance of‘HF'and‘GD'apple to AAAP ***,RNL,and JA signal-related genes were upregulated in both cultivars to restrict AAAP ***,genes encoding CNLs,TNLs,WRKYs,and AP2s were only activated in‘HF'as part of the resistance response,of which,some play major roles in the regulation of ET and SA signal *** analysis showed that many DEGs with opposite expression trends in the two hosts may play important regulatory roles in response to AAAP *** expression of one such gene MdERF110 in‘GD'apple leaves improved AAAP ***,this study highlights the reasons for differential resistance to AAAP infection between‘HF'and‘GD'apples which can theoretically assist the molecular breeding of disease-resistant apple crops.

关键词： Apple Transcriptome Alternaria alternata apple pathotype Infection defense response

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BnaWRKY75 positively regulates the resistance against Sclerotinia sclerotiorum in ornamental Brassica napus

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Horticultural Plant Journal 2024年第3期10卷 784-796页

作者： Kexin Yu Yijie Zhang Xiaoyuan Fei Luyue Ma Rehman Sarwar Xiaoli Tan Zheng Wang School of Life Sciences Jiangsu UniversityZhenjiangJiangsu 212013China School of Food and Biological Engineering Jiangsu University ZhenjiangJiangsu 212013China Key Laboratory for Theory and Technology of Intelligent Agricultural Machinery and Equipment Jiangsu UniversityZhenjiangJiangsu 212013China

With the development of tourism at home and abroad,Rapeseed(Brassica napus)has become an important ornamental ***,its ornamental value at the inflorescence stage is greatly reduced by Sclerotinia *** of important genes in the defense responses is critical for molecular breeding,which is an important strategy for controlling the *** this study,we isolated a *** WRKY transcription factor gene,***75 was found to encode a nucleus-localized protein and exhibited relatively high expression in the *** thaliana transgenic plants expressing BnaWRKY75 showed enhanced resistance to ***,and both ProBnaWRKY75:GUS and gene expression analyses showed that BnaWRKY75 was highly responsive to *** infection,indicating the involvement of BnaWRKY75 in response to this ***,overexpression(OE)of BnaWRKY75 in *** significantly enhanced the resistance to ***,whereas the resistance was reduced in RNAi transgenic *** ***,the BnaWRKY75-OE *** plants exhibited constitutive activation of salicylic acid-,jasmonic acid-,and ethylene-mediated defense responses and the inhibition of both H_(2)O_(2)and O_(2)·^(-)accumulation in response to this *** contrast,BnaWRKY75-RNAi plants showed a reverse pattern,suggesting that BnaWRKY75 is involved in hormonal signaling pathways and in the control of reactive oxygen species *** conclusion,these data indicate that BnaWRKY75,a regulator of multiple defense responses,positively regulates resistance against ***,which may guide the improvement of resistance in rapeseed.

关键词： Brassica napus BnaWRKY75 defense response Sclerotinia sclerotiorum

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iTRAQ-based quantitative proteomics analysis of defense responses triggered by the pathogen Rhizoctonia solani infection in rice

iTRAQ-based quantitative proteomics analysis of defense resp...

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江苏省遗传学会2019年学术研讨会

作者：冯志明 ZHAO Jianhua ZHANG Huimin ZHANG Yafang CHEN Zongxiang 左示敏 Yangzhou University

The soil-borne necrotrophic fungus Rhizoctonia solani（R. solani） is destructive, leading to drasticyield losses in various important crops. However, the host defense mechanism in response to invasion of thispathogen are poorly understood. In our study, an i TRAQ-based quantitative proteomic analysis was employed to investigate R. solani-responsive proteins in the disease resistant rice cultivar YSBR1. As awhole, 319 differentially accumulated proteins（DAPs） were identified after inoculation of rice plants withR. solani. Functional categorization analysis indicated that these DAPs covered a broad range of ***, a substantial portion of DAPs were involved in cell redox homeostasis, carbohydrate metabolism, phenylpropanoid biosynthesis and pathogenesis-related proteins, indicating that these processes are important for rice defense against R. solani. Additionally, a little part of proteins involved in photosynthesisand chlorophyll biosynthetic process, and phenylpropanoid biosynthesis were markedly down-accumulatedin response to R. solani infection, implying that R. solani probably attacks photosynthetic system of riceplants to cause disease, and inhibits phenylpropanoid biosynthesis to facilitate their infection andcolonization. In conclusion, our results suggest that the defense response of rice plants to R. solani invasionis wide-ranging and active and involves the induction of proteins from various innate immune-relatedpathways.

关键词： rice sheath blight Rhizoctonia solani proteomic defense response

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Cardinium infection alters cotton defense and detoxification metabolism of its whitefly host

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Insect Science 2023年第2期30卷 473-485页

作者： Ying Liu Kun Yang Jicheng Wang Dong Chu Shandong Engineering Research Center for Environment-Friendly Agricultural Pest Management College of Plant Health and MedicineQingdao Agricultural UniversityQingdaoShangdong ProvinceChina

Field monitoring revealed that the infection ratio of the bacterial symbiont Cardinium in the whitefly(Bemisia tabaci MED)was relatively low in northern ***,the role of this symbiont and the symbiont-whitefly-host plant interaction mechanism are poorly *** investigated the influence of Cardinium on the competitiveness of the host whitefly and the physiological interaction between the host plants and host ***-infected whiteflies were displaced by uninfected whiteflies after 5 generations,which showed that Cardinium infection reduced whitefly *** defense response genes of cotton significantly decreased under infestation by infected whiteflies compared to uninfected *** expression of detoxification metabolism genes,especially the uridine 5ʹ-diphospho-glucuronyltransferase and P450 genes,in infected whiteflies significantly *** results demonstrated that Cardinium could inhibit the defense response of the host plant and decrease the detoxification metabolism ability of the host *** reduced competitiveness of infected whiteflies may be associated with the inhibition of the whitefly detoxification metabolism by Cardinium,resulting in the reduced performance of infected ***,Cardinium infection can suppress plant defenses,which may benefit both infected and uninfected whiteflies when they *** research illustrates the symbiont–whitefly–host plant interaction mechanism and the population dynamics of the whitefly.

关键词： Bemisia tabaci MED Cardinium defense response detoxification metabolism

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Jasmonic Acid is Induced in a Biphasic Manner in response of Pea Seedlings to Wounding

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Journal of Integrative Plant Biology 2009年第6期51卷 562-573页

作者： Hao-Ru Yang Ke Tang Hong-Tao Liu Qiu-Hong Pan Wei-Dong Huang College of Food Science and Nutritional Engineering China Agricultural University Beijing 100083 China Institute of Geographic Sciences and Natural Resources Research the Chinese Academy of Sciences Beijing 100101 China

The role of jasmonic acid （JA） in plant wounding response has been demonstrated. However, the source of JA in wound signaling remains unclear. In the present study, pea seedlings were used as material to investigate the systemic induction of JA and the activation of lipoxygenase （LOX）-dependent octadecanoid pathway upon wounding. The results showed that endogenous JA could induce two peaks in the wounded leaves and the stalks, while only one peak in the systemic leaves. LOX activity and its protein amount were also induced and the stimulation mainly occurred in the late phase, while one peak of induction was present after pretreatment with JA. Applied nordihydroguaiaretic acid （NDGA）, an inhibitor of LOX activity, only inhibited the induction of JA in the late phase, and the resistance of pea was impaired. Furthermore, 13（S）- hydroperoxy-9（Z）, 11 （E）-octadecadienoic acid （13（S）-H（P）ODE） was confirmed to be the main product of LOX throughout the experimental time. in addition, immunocytochemical analysis also revealed the occurrence of JA biosynthesis and transport upon wounding. These results demonstrated that wound-induced JA in wounded leaves resulted from its biosynthesis and conversion from its conjugates, while in systemic leaves resulted from its transport and biosynthesis; and proved that the LOX pathway was vital to the wound-induced defense response involved in JA biosynthesis.

关键词： defense response jasmonic acid lipoxygenase pea (Pisum sativum L.).

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The jasmonate-induced bHLH gene SlJIG functions in terpene biosynthesis and resistance to insects and fungus

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Journal of Integrative Plant Biology 2022年第5期64卷 1102-1115页

作者： Yunyun Cao Lun Liu Kangsheng Ma Wenjing Wang Hongmei Lv Ming Gao Xinman Wang Xichun Zhang Shuxin Ren Na Zhang Yang-Dong Guo College of Horticulture China Agricultural UniversityBeijing 100193China College of Horticulture Science and Engineering Shandong Agricultural UniversityTai'an 271018China Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhan 430070China College of Plant Science and Technology Beijing University of AgricultureBeijing 102206China School of Agriculture Virginia State UniversityPetersburg 23806VAUSA Sanya Institute of China Agricultural University Sanya 572000China

Jasmonic acid(JA)is a key regulator of plant defense *** the transcription factor MYC2,the master regulator of the JA signaling pathway,orchestrates a hierarchical transcriptional cascade that regulates the JA responses,only a few transcriptional regulators involved in this cascade have been ***,we identified the basic helix-loop-helix(bHLH)transcription factor gene in tomato(Solanum lycopersicum),METHYL JASMONATE(MeJA)-INDUCED GENE(SlJIG),the expression of which was strongly induced by MeJA *** and molecular biology experiments revealed that SlJIG is a direct target of *** knockout plants generated by gene editing had lower terpene contents than the wild type from the lower expression of TERPENE SYNTHASE(TPS)genes,rendering them more appealing to cotton bollworm(Helicoverpa armigera).Moreover,SlJIG knockouts exhibited weaker JA-mediated induction of TPSs,suggesting that SlJIG may participate in JA-induced terpene *** out SlJIG also resulted in attenuated expression of JA-responsive defense genes,which may contribute to the observed lower resistance to cotton bollworm and to the fungus Botrytis *** conclude that SlJIG is a direct target of MYC2,forms a MYC2-SlJIG module,and functions in terpene biosynthesis and resistance against cotton bollworm and ***.

关键词： basic helix-loop-helix transcription factor defense response jasmonic acid MYC2 terpene biosynthesis

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Comparative proteomic profiles of resistant/susceptible cucumber leaves in response to downy mildew infection

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Horticultural Plant Journal 2021年第4期7卷 327-340页

作者： Chengzhen Sun Xiaofei Song Jinshuang Zheng Xiaoli Li Zhihong Feng Liying Yan Hebei Key Laboratory of Horticultural Germplasm Excavation and Innovative Utilization QinhuangdaoHebei 066004China College of Horticulture Science and Technology Hebei Normal University of Science and TechnologyQinhuangdaoHebei 066004China

Downy mildew is a serious disease in cucumber production worldwide,which is caused by Pseudoperonospora cubensis(Berk.&Curt.)*** the mechanism of cucumber response to downy mildew infection is important for breeding improvement and production;however,the research remains largely elusive.A comparative proteomic approach was used to reveal the differential accumulation of the proteomes from leaves of cucumber(susceptible line and resistant line)that were inoculated with *** or not by two-dimensional electrophoresis and MALDI-TOF/TOF MS.A total of 76 protein spots were successfully identified with significant changes in abundance(>2-fold,Pdefense(17%),and protein biosynthesis,folding and degradation(13%),unclassified(7%),nucleotide metabolism(3%),signal transduction(1%)and cellular process(1%).Among the 17 differentially expressed proteins between the resistant and susceptible cucumber line,most of the protein spots were concentrated in cell rescue and defense(4)and energy and metabolism(4).Moreover,a schematic diagram containing majority of the metabolic pathways of cucumber leaves in response to downy mildew was *** network revealed the positive effect of several functional components in cucumber seedlings’resistant to downy mildew such as accumulation of energy supply and resistance-related proteins,hastened protein metabolism and photorespiratory,inhibited photosynthesis,and triggered photosystem repair and programmed cell *** together,these results have advanced a further understanding of the key metabolic pathways of cucumber resistance to downy mildew and pathogen control in the proteomic level.

关键词： defense response Downy mildew Proteomics Cucumber

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OsWRKY03, a rice transcriptional activator that functions in defense signaling pathway upstream of OsNPR1

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Cell Research 2005年第8期15卷 593-603页

作者： Xiao Qiang LIU1,2, Xian Quan BAI1,2, Qian QIAN3, Xiu Jie WANG1, Ming Sheng CHEN1, Cheng Cai CHU1,* 1National Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China 2Graduate School of the Chinese Academy of Sciences, Yuquan Road, Beijing 100039, China 3China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310006, China National Key Laboratory of Plant Genomics Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing China Graduate School of the Chinese Academy of Sciences Yuquan Road Beijing China China National Rice Research Institute Chinese Academy of Agricultural Sciences Hangzhou China

WRKY family proteins are a class of plant specific transcription factors that involve in many stress response pathways. It has been shown that one Arabidopsis WRKY protein, AtWRKY29/22, is activated by MAP kinase signaling cascade and confers resistance to both bacterial and fungal pathogens. However, little is known about the biological roles of WRKY proteins in rice. In this study, we investigated the expression patterns of rice AtWRKY29/22 homolog, OsWRKY03, under different conditions, and also its possible role involved in plant defense. Our results showed that OsWRKY03 was up-regulated by several defense signaling molecules or different treatments. Further analysis revealed that the expres- sion of OsWRKY03 was light dependent. Transcriptional activation activity of OsWRKY03 was also demonstrated by yeast functional assay. Transient expression of OsWRKY03-GFP fusion protein in onion epidermis cells showed that OsWRKY03 was a nuclear localized protein. OsNPR1 as well as several other pathogenesis-related genes, such as OsPR1b, phenylalanine ammonia-lyase (ZB8) and peroxidase (POX22.3), were induced in OsWRKY03-overexpressing transgenic plants. These results indicated that OsWRKY03 is located upstream of OsNPR1 as a transcriptional activator in salicylic acid (SA)-dependent or jasmonic acid (JA)-dependent defense signaling cascades.

关键词： defense response OsWRKY03 transcription factor rice.

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