Comparative proteomic profiles of resistant/susceptible cucumber leaves in response to downy mildew infection

作     者：Chengzhen Sun Xiaofei Song Jinshuang Zheng Xiaoli Li Zhihong Feng Liying Yan Chengzhen Sun;Xiaofei Song;Jinshuang Zheng;Xiaoli Li;Zhihong Feng;Liying Yan

作者机构：Hebei Key Laboratory of Horticultural Germplasm Excavation and Innovative UtilizationQinhuangdaoHebei 066004China College of Horticulture Science and TechnologyHebei Normal University of Science and TechnologyQinhuangdaoHebei 066004China 

出 版 物：《Horticultural Plant Journal》 (园艺学报（英文版）)

年 卷 期：2021年第7卷第4期

页      面：327-340页

核心收录：

学科分类：09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 090402[农学-农业昆虫与害虫防治] 

基　　金：This work was supported by the Hebei Province Scientific and Technological Support Program(Grant No.16226308D-4) Hebei Vegetable Innovation Projects of Modern Agricultural Industry Technology System(Grant No.HBCT2018030209) Hebei Province High Education Science and Technology Research Project(Grant No.BJ2018002) 

主　　题：Defense response Downy mildew Proteomics Cucumber 

摘      要：Downy mildew is a serious disease in cucumber production worldwide,which is caused by Pseudoperonospora cubensis(Berk.&Curt.)*** the mechanism of cucumber response to downy mildew infection is important for breeding improvement and production;however,the research remains largely elusive.A comparative proteomic approach was used to reveal the differential accumulation of the proteomes from leaves of cucumber(susceptible line and resistant line)that were inoculated with *** or not by two-dimensional electrophoresis and MALDI-TOF/TOF MS.A total of 76 protein spots were successfully identified with significant changes in abundance(2-fold,P0.05)in downy mildew infected or not leaves for the susceptible line and resistant *** the functional annotation,these proteins were classified into 8 groups including photosynthesis(29%),energy and metabolism(29%),cell rescue and defense(17%),and protein biosynthesis,folding and degradation(13%),unclassified(7%),nucleotide metabolism(3%),signal transduction(1%)and cellular process(1%).Among the 17 differentially expressed proteins between the resistant and susceptible cucumber line,most of the protein spots were concentrated in cell rescue and defense(4)and energy and metabolism(4).Moreover,a schematic diagram containing majority of the metabolic pathways of cucumber leaves in response to downy mildew was *** network revealed the positive effect of several functional components in cucumber seedlings’resistant to downy mildew such as accumulation of energy supply and resistance-related proteins,hastened protein metabolism and photorespiratory,inhibited photosynthesis,and triggered photosystem repair and programmed cell *** together,these results have advanced a further understanding of the key metabolic pathways of cucumber resistance to downy mildew and pathogen control in the proteomic level.