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Genetic Diversity,Antibiotic Resistance,and Pathogenicity of Aeromonas Species from Food Products in Shanghai,China

Biomedical and Environmental Sciences 2022年第9期35卷 842-853页

作者： QU Feng Tian WANG Wen Qing LIU Qian ZHOU Hai Jian HU Jin Rui DU Xiao Li WANG Yue XUE Jia Qi CUI Zhi Gang XIE Gui Lin MENG Shuang College of Life Science Northeast Agricultural UniversityHa’erbin 150000HeilongjiangChina State Key Laboratory of Infectious Disease Prevention and Control National Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China Shanghai Pudong New Area Center for Disease Control and Prevention Shanghai 200136China Laboratory Medicine Beijing HospitalNational Center of GerontologyInstitute of Geriatric MedicineChinese Academy of Medical SciencesBeijing 100730China Clinic Medical College North China University of Science and TechnologyTangshan 063000HebeiChina

Objective Aeromonas has recently been recognized as an emerging human pathogen.Aeromonasassociated diarrhea is a phenomenon occurring worldwide.This study was designed to determine the prevalence,genetic diversity,antibiotic resistance,and pathogenicity of Aeromonas strains isolated from food products in Shanghai.Methods Aeromonas isolates(n=79)collected from food samples were analyzed using concatenated gyrB-cpn60 sequencing.The antibiotic resistance of these isolates was determined using antimicrobial susceptibility testing.Pathogenicity was assessed usingβ-hemolytic,extracellular protease,virulence gene detection,C.elegans liquid toxicity(LT),and cytotoxicity assays.Results Eight different species were identified among the 79 isolates.The most prevalent Aeromonas species were A.veronii[62(78.5%)],A.caviae[6(7.6%)],A.dhakensis[3(3.8%)],and A.salmonicida[3(3.8%)].The Aeromonas isolates were divided into 73 sequence types(STs),of which 65 were novel.The isolates were hemolytic(45.6%)and protease-positive(81.0%).The most prevalent virulence genes were act(73.4%),fla(69.6%),aexT(36.7%),and ascV(30.4%).The results of C.elegans LT and cytotoxicity assays revealed that A.dhakensis and A.hydrophila were more virulent than A.veronii,A.caviae,and A.bivalvium.Antibiotic resistance genes[tetE,blaTEM,tetA,qnrS,aac(6)-Ib,mcr-1,and mcr-3]were detected in the isolates.The multidrug-resistance rate of the Aeromonas isolates was 11.4%,and 93.7%of the Aeromonas isolates were resistant to cefazolin.Conclusion The taxonomy,antibiotic resistance,and pathogenicity of different Aeromonas species varied.The Aeromonas isolates A.dhakensis and A.hydrophila were highly pathogenic,indicating that food-derived Aeromonas isolates are potential risks for public health and food safety.The monitoring of food quality and safety will result in better prevention and treatment strategies to control diarrhea illnesses in China.

关键词： Aeromonas Genetic diversity Antibiotic resistance Virulence gene cytotoxicity assay Multidrug-resistance

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Hydrothermal synthesis of pyrrhotite(Fex-1S) nanoplates and their antibacterial, cytotoxic activity study

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Progress in Natural Science:Materials International 2018年第4期28卷 447-455页

作者： Liliana Argueta-Figueroa Nayely Torres-Gómez René García-Contreras A.R.Vilchis-Nestor O.Martínez-Alvarez L.S.Acosta-Torres M.C.Arenas-Arrocena Laboratorio de Investigación Interdisciplinaria área de Nanoestructuras y Biomateriales Escuela Nacional de Estudios Superiores Unidad León Universidad Nacional Autónoma de México Centro Conjunto de Investigación en Química Sustentable (CCIQS) UEAMex-UNAM Departamento de Ingeniería en Energía Universidad Politécnica de Guanajuato

The aim of this study was to synthesize and characterize Fex-1S 2D-nanostructures with pyrrhotite phase,as well as to explore their biological(antibacterial and cytotoxic)properties,namely the expression of reactive oxygen species(ROS)in the exposure of cells and bacteria.Based on hydrothermal synthesis,the characterization of asprepared 2D-nanostructures was performed by XRD,SEM,EDS,and TEM,in which the single-crystalline pyrrhotite phased Fex-1S nanoplate morphology was observed.The antibacterial activities of Fex-1S nanoplates against human pathogenic strains such as Staphylococcus aureus,Escherichia coli,and Enterococcus faecalis were tested.Minimum inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)were determined following the broth microdilution method.cytotoxicity and expression of intracellular ROS of pyrrhotite nanoplates on Human Gingival Fibroblast(HGF),Human Pulp Cells(HPC)and Human Osteoblast(HBC)were calculated.Cell viability was determined by the MTT method.All experiments were performed of three independent experiments and data were analyzed by Kruskal-Wallis and Mann-Whitney tests,also Pearson′s Correlation was performed.The nanoplates exhibited good bactericidal effect.All types of cells tested showed slight cytotoxicity.It was found that intracellular ROS is produced when cells and bacteria tested are exposed to pyrrhotite nanoplates in presence of both air and peroxide hydrogen.ROS production levels were higher in the bacteria than the cells exposed to these nanoplates.

关键词： Hydrothermal synthesis Iron sulfide nanoplates Minimal inhibitory concentration Antibacterial effect cytotoxicity assay

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Antioxidant,cytotoxic,and anti-venom activity of Alstonia parvifolia Merr.Bark

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Asian Pacific Journal of Tropical Biomedicine 2021年第10期11卷 460-468页

作者： Maria Carmen S.Tan Mary Stephanie S.Carranza Virgilio C.Linis Raymond S.Malabed Yves Ira A.Reyes Francisco C.Franco,Jr. Glenn G.Oyong Chemistry Department De La Salle University2401 Taft AvenueManila 1004Philippines Biology Department De La Salle University2401 Taft AvenueManila 1004Philippines Department of Chemistry Graduate School of ScienceOsaka UniversityOsaka 563-0034Japan Molecular Science Unit Laboratory Center for Natural Sciences and Environmental ResearchDe la Salle University2401 Taft AvenueManila 1004Philippines

Objective:To evaluate antioxidant,cytotoxic,and anti-venom capacity of crude bark extracts of Alstonia parvifolia Merr.Methods:Gas chromatography-mass spectrometry(GC-MS)and energy dispersive X-ray analyses were accomplished to characterize the chemical constituents of Alstonia parvifolia.Biochemical characterization was evaluated using an inhibitory phospholipase A_(2)(PLA_(2))assay,DPPH,and cytotoxicity assays.Using the constituents listed in the GC-MS analyses,molecular docking was conducted to inspect the binding energies between the chosen compounds and selected PLA_(2) isoforms.Results:GC-MS analyses showed that the Alstonia parvifolia crude extract consisted predominantly of acetylmarinobufogenin(14.89%),γ-sitosterol(10.44%),3-O-methyl-D-glucose(5.88%),3,5-dimethoxy-4-hydroxyphenylacetic acid(5.30%),(2α,5α)-17-methoxyaspidofractinin-3-one(AFM)(4.08%),and 2,3,5,6,7,8,9-heptahydro-1-phenyl-5-(p-chlorophenylimino)-1H-benzo[e][1,4]thiazepine(HPT)(1.37%).The principal elemental components of Alstonia parvifolia were Ca(4.012%)and K(1.496%),as exhibited by energy dispersive X-ray examination.Alstonia parvifolia showed significant free radical scavenging ability(IC_(50):0.287 mg/mL)and was non-cytotoxic to normal HDFn cells(IC_(50)>100μg/mL).Moreover,Alstonia parvifolia was favorably cytotoxic to MCF-7(IC_(50):4.42µg/mL),followed by H69PR,HT-29,and THP-1,with IC_(50) values of 4.94,5.07,and 6.27µg/mL,respectively.Alstonia parvifolia also displayed notable inhibition against PLA_(2) activity of Naja philippinensis Taylor venom with IC_(50) of(15.2±1.8)μg/mL.Docking and cluster analyses projected negative binding energies from AFM(−6.36 to−9.68 kcal/mol),HPT(−7.38 to−9.77 kcal/mol),and acetylmarinobufogenin(−7.22 to−9.59 kcal/mol).These calculations were for the particular interactions of Alstonia parvifolia constituents to PLA_(2) homologues where the utmost affinity was detected in HPT owing to the dipole interactions with amino acid residues.Conclusions:The bark extract of Alstonia parvifolia shows great potent

关键词： Alstonia parvifolia Merr. Naja philippinensis Taylor Gas chromatography-electron ionization-mass spectrometry Secretory phospholipase A_(2) cytotoxicity assay Anti-venom

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Purification and bioactive analysis of a recombinant scorpion insect toxin BmK IT

Purification and bioactive analysis of a recombinant scorpio...

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第九届中国生物毒素学术研讨会

作者： Renjia Yang Jun Du Yuejun Fu Aihua Liang (Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education,Institute of Biotechnology,Shanxi University,Taiyuan,030006,PR China)

＜正＞The scorpion venoms are a rich source of toxic peptides.BmK IT is an excitatory scorpionβ-toxin in Buthus martens ii Karsch which is a sort of scorpion mainly distributed in our country.This toxin is composed of 69 amino acids residues cross-linked by four disulfide bridges.In this study,the toxin was purified and characterized by in vitro studies.

关键词： BmK IT Purification cytotoxicity assay Electrophysiology

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