Purification and bioactive analysis of a recombinant scorpion insect toxin BmK IT

作     者：Renjia Yang Jun Du Yuejun Fu Aihua Liang (Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education,Institute of Biotechnology,Shanxi University,Taiyuan,030006,PR China) 

会议名称：《第九届中国生物毒素学术研讨会》

会议日期：2009年

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：supported by grants from“National Natural Science Foundation of China”(No.30700534) “Natural Science Foundation of Shanxi Province”(2008021039) 

关 键 词：BmK IT Purification Cytotoxicity assay Electrophysiology 

摘      要：正The scorpion venoms are a rich source of toxic *** IT is an excitatory scorpionβ-toxin in Buthus martens ii Karsch which is a sort of scorpion mainly distributed in our *** toxin is composed of 69 amino acids residues cross-linked by four disulfide *** this study,the toxin was purified and characterized by in vitro studies.