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No consistent daily variation in DNA methylation detected in Populus nigra leaves by methylation-sensitive amplification polymorphism analysis

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Journal of Forestry Research 2017年第4期28卷 653-660页

作者： Shu Diao Yanbo Wang Changjun Ding Yingying Chang Lixiong Liang Yanan Gao Bingyu Zhang Xiaohua Su State Key Laboratory of Tree Genetics and Breeding Research Institute of ForestryChinese Academy of ForestryBeijing 100091People’s Republic of China Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration Research Institute of ForestryChinese Academy of ForestryBeijing 100091People’s Republic of China

DNA methylation, an epigenetic mechanism used by cells to control gene expression, has an important biological role in plant development and environmental fitness. Since plant DNA methylation is closely related to environmental conditions, variation during the day is expected. Here, in genetically identical plants of Populus nigra clone N46, DNA methylation changes in leaves over a 24 h period were detected using the methylation-sensitive amplification polymorphism method. The results showed different DNA methylation patterns in mature poplar leaves: not only in individuals at the same time, but also in samples at each of the six time during the day. In addition, night samples had a higher percentage of methylation than in morning samples. However, no statistically significant differences were found among the samples gathered at different times. Similar results were obtained for three other P. nigra clones with different genetic backgrounds. Real time qPCR showed that the DNA methyltransferase genes Pt-MET1 and Pt-SOM1 involved in CG DNA methylation in poplar were stable over a 24 h period in leaves of P. nigra N46 compared with circadian-controlled genes. That could be part of the reason that methylation of CCGG sites is stable in those leaves. That DNA methylation differed even in genetically identical plants indicates the specificity of DNA methylation changes in their genomes. No statistically significant differences in methylation changes were found between day and night, suggesting that DNA methylation is more stable than expected and is unlikely to be involved in circadian regulation in plants.

关键词： cytosine methylation Epigenetic Populus nigra methylation-sensitive amplification polymorphism (MSAP) Variation

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Study on DNA methylation Patterns of NB-LRR Family Encoding Genes in Arabidopsis thaliana

Study on DNA Methylation Patterns of NB-LRR Family Encoding ...

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中国植物病理学会2016年学术年会

作者： KONG Wei-wen LI Bin School of Horticulture and Plant Protection Yangzhou University

Nucleotide-binding site leucine-rich repeat（NB-LRR） proteins in plants constitute a large family and play very important roles in disease *** pivotal problems how NB-LRR genes in plants exist and how they are regulated at transcriptional level remain to be *** DNA methylation is a pervasive and conservative epigenetic mark in eukaryotes,and it is important in regulation of *** this study,NB-LRR encoding genes in model plant Arabidopsis thaliana（Columbia ecotype）are selected for study on the DNA methylation patterns in the wild type and mutants whose original genes are key factors regulating DNA *** on the results,this study explores further the issues between changes for gene expression at transcriptional levels and changes for DNA methylation *** main results are as *** vast majority of NB-LRR encoding genes in *** are modified by DNA *** methylation can be found in the promoters as well as the gene bodies for the most NB-LRR encoding genes,and the main methylation type is CG ***,there are three members including At1g58807,At1g59124 and At1g59218 without DNA methylation *** of key regulators AGO4,MET1,CMT3,DRM1/2 and DDM1 function separately leads to decrease of the methylation counts of NB-LRR encoding *** methylation levels of gene bodies of NB-LRR family in *** are higher than those of 500- bp and 1000- bp *** are observed in the wild type and almost all the mutants,especially in the CG type *** average of methylation levels of NB-LRR encoding genes changes regularly according to the different regions of *** genes encoded by NB-LRR family in *** are likely to be modulated by altered DNA methylation *** of transcriptome data from wild type and met1,cmt3,drm1/2 and ddm1 mutants reveals that differences at transcription levels between wild type and mutants are significant f

关键词： Arabidopsis thaliana NB-LRR encoding genes cytosine methylation methylation pattern Gene regulation

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Genetic and Epigenetic Diversities Shed Light on Domestication of Cultivated Ginseng （Panax ginseng）

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Molecular Plant 2015年第11期8卷 1612-1622页

作者： Ming-Rui Li Fena-Xue Shi Yu-Xin Zhou Ya-Ling Li Xin-Feng Wang Cui Zhang Xu-Tong Wang Bao Liu Hong-Xing Xiao Lin-Feng Li Key Laboratory of Molecular Epigenetics of Ministry of Education Northeast Normal University #5268 Renmin Street Changchun 130024 China Institute of Grassland Science Northeast Normal University Key Laboratory of Vegetation Ecology Ministry of Education Changchun 130024 China

Chinese ginseng （Panaxginseng） is a medically important herb within Panax and has crucial cultural values in East Asia. As the symbol of traditional Chinese medicine, Chinese ginseng has been used as a herbal remedy to restore stamina and capacity in East Asia for thousands of years. To address the evolutionary origin and domestication history of cultivated ginseng, we employed multiple molecular approaches to investigate the genetic structures of cultivated and wild ginseng across their distribution ranges in northeastern Asia. Phylogenetic and population genetic analyses revealed that the four cultivated ginseng landraces, COMMON, BIANTIAO, SHIZHU, and GAOLI （also known as Korean ginseng）, were not domesticated independently and Fusong Town is likely one of the primary domestication centers. In addition, our results from population genetic and epigenetic analyses demonstrated that cultivated ginseng maintained high levels of genetic and epigenetic diversity, but showed distinct cytosine methylation patterns compared with wild ginseng. The patterns of genetic and epigenetic variation revealed by this study have shed light on the domestication history of cultivated ginseng, which may serve as a framework for future genetic improvements.

关键词： Panax ginseng domestication traditional Chinese medicine cytosine methylation genetic and epigenetic diversity

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Epigenetic modulation of Wnt signaling contributes to the neuropathic pain in the rats

Epigenetic modulation of Wnt signaling contributes to the ne...

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中国中西医结合麻醉学会[CSIA]年会暨第二届全国中西医结合麻醉学术研讨会、江苏省中西医结合学会麻醉专业委员会成立大会

作者： Wei Feng Haichen Chu Ruynag Teng Yang Zhao Jie Gao Department of Anaesthiology Affiliated Hospital of Qingdao University

Emerging reports demonstrated that Wnt/β-catenin pathways critically contributed to the induction and maintenance of chronic pain induced by peripheral inflammation and nerve *** evidences also suggested the critical role of epigenetic mechanism in the setting of chronic *** present study aims to elucidate the potential epigenetic mechanism underlying the altered Wnt signaling and its functional significance in the rats with neuropathic pain induced by chronic constriction injury(CCI) in sciatic *** the present study significantly increased expression of Wnt3 a was observed in the dorsal horn of the rats with *** was also found that significantly increased histone H3 acetylation and decreased cytosine methylation in the promoter region of Wntia in the dorsal horn of the rats with *** application of XAV939,an inhibitor of Wnt signaling,significantly decreased the expression of the active P-catenin and attenuated the painful behavioral response to thermal and mechanical stimuli in the rat with *** results demonstrated an epigenetic upregulation of Wnt3 a in the dorsal horn,which critically contributed to the maintenance of painful behavior in the rats with neuropathic pain.

关键词： neuropathic pain Wnt histone acetylation cytosine methylation

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