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A comparative Study of Human and Animal Hairs:Microscopic Hair comparison and cytochrome c oxidase i Species identification

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Journal of Forensic Science and Medicine 2019年第1期5卷 20-23页

作者： Venusia cortellini Anna carobbio Gloria Brescia Nicoletta cerri Andrea Verzeletti Department of Medical and Surgical Specialties Radiological Sciences and Public HealthForensic Medicine UnitUniversity of BresciaBresciaItaly

Human and animal hairs have been used in forensic investigations for over a *** is stable under adverse natural conditions;hence,it is often recovered at the crime scene,and it is necessary to determine whether the hair is of human or animal *** and genetic characteristics are useful to differentiate human hair from animal *** the present study,we analyzed the distinguishing characteristics of hair of various *** addition,we explore species identification by cytochrome c oxidase i mitochondrial gene *** confirm that both the microscopic and molecular analyses of hairs are useful in forensic investigations.

关键词： cytochrome c oxidase i hair optical microscope species identification

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identifying chinese species of Gammarus(crustacea:Amphipoda) using DNA barcoding

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current Zoology 2009年第2期55卷 158-164页

作者： Zhong-e HOU Zhu Li Shu-qiang Li institute of Zoology Chinese Academy of Sciences Beijing 100101 China Beijing Museum of Natural History Beijing 100050 China

Using a standard cytochrome c oxidase i sequence,DNA barcoding has been shown to be effective to distinguish known species and to discover cryptic species. Here we assessed the efficiency of DNA barcoding for the amphipod genus Gammarus from china. The maximum intraspecific divergence for widespread species,Gammarus lacustris,was 3.5%,and mean interspecific divergence reached 21.9%. We presented a conservative benchmark for determining provisional species using maximum intraspecific divergence of Gammarus lacustris. Thirty-one species possessed distinct barcode clusters. Two species were comprised of highly divergent clades with strong neighbor-joining bootstrap values,and likely indicated the presence of cryptic species. Although DNA barcoding is effective,future identification of species of Gammarus should incorporate DNA barcoding and morphological detection.

关键词： Gammarus cytochrome c oxidase i DNA barcoding cryptic species Taxonomy

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Molecular identification of the potentially forensically relevant cluster flies Pollenia rudis (Fabricius) and Pollenia vagabunda (Meigen) (Diptera: Polleniidae) — non-recorded species in Algeria

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Forensic Sciences Research 2022年第1期7卷 69-77页

作者： Meriem Taleb Ghania Tail Halide Nihal Açıkgöz Department of Population and Organism Biology University of Blida 1BlidaAlgeria Department of Forensic Biology Ankara UniversityMamakAnkaraTurkey

cluster flies are represented by the genus Pollenia Robineau-Desvoidy,1830 of the family Polleniidae Brauer and Bergenstamm,*** larvae are known to be internal parasites or predators of ***,we report for the first time the occurrence of the cluster flies Pollenia rudis Fabricius,1794 and Pollenia vagabunda(Meigen,1826)(Diptera:Polleniidae)on carcasses in Algeria and identify them through DNA barcoding.A region of the mitochondrial cytochrome c oxidase i gene(cOi)was amplified and *** distances were determined.A phylogenetic tree was constructed with the maximum parsimony method using 10000 bootstrap replicates.A total number of 157 adults of *** were collected together with 325 adults of Pollenia *** occurrence of Pollenia on animal carcasses does not seem to be correlated with a particular stage of *** the sequences were correctly identified using the BLASTn tool from the GenBank database and the BOLD identification ***-and interspecific sequence divergence values were less than 1%and greater than 3%,*** barcodes obtained from this study were robust enough to identify and distinguish unambiguously between *** and *** the tree-based analysis,the cluster flies were all assigned to their respective species separately from each other confirming the morphological *** results provide DNA barcodes that contribute to the growth of reference databases and allow fast and accurate identification.

关键词： Forensic sciences forensic entomology Pollenia cytochrome c oxidase i DNA barcoding North Africa

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