检索结果-南通市图书馆

cystic fibrosis transmembrane conductance regulator prevents ischemia/reperfusion induced intestinal apoptosis via inhibiting PI3K/AKT/NF-κB pathway

引用

World Journal of Gastroenterology 2022年第9期28卷 918-932页

作者： Zhi-Wei Dong Hui Liu Fei-Fei Su Xiao-Zhou Fan Yong Zhang Peng Liu Department of General Surgery Air Force Medical CenterBeijing 100000China Department of Gastroenterology Second Affiliated Hospital of Dalian Medical UniversityDalian 116023Liaoning ProvinceChina Department of Cardiology Air Force Medical CenterBeijing 100000China Department of Ultrasound Air Force Medical CenterBeijing 100000China School of Chemistry and Biological Engineering University of Science and TechnologyBeijing 100000China Research Laboratory of Aero-Medical Support Air Force Medical CenterBeijing 100000China

BACKGROUND Intestinal ischemia/reperfusion(I/R)injury is a fatal syndrome that occurs under many clinical *** apoptosis of intestinal cells caused by ischemia can cause cell damage and provoke systemic dysfunction during ***,the mechanism of I/R-induced apoptosis remains *** fibrosis transmembrane conductance regulator(CFTR)is a cAMP-activated chloride *** researchers have paid attention to its role in intestinal I/R injury,or the relationship between CFTR and intestinal apoptosis induced by hypoxia/reoxygenation(H/R).AIM To investigate the effects of CFTR on I/R-induced intestinal apoptosis and its underlying molecular *** An intestinal I/R injury model was established in mice with superior mesenteric artery occlusion, and Caco2 cells were subjected to H/R for the simulation of I/R in *** results suggested that CFTR overexpression significantly increased the Caco2 cell viability anddecreased cell apoptosis induced by the H/R. Interestingly, we found that the translocation of p65,an NF-κB member, from the cytoplasm to the nucleus after H/R treatment can be reversed by theoverexpression of CFTR, the NF-κB P65 would return from the nucleus to the cytoplasm asdetermined by immunostaining. We also discovered that CFTR inhibited cell apoptosis in theH/R-treated cells, and this effect was significantly curbed by the NF-κB activator BA, AKTinhibitor GSK690693 and the PI3K inhibitor LY294002. Moreover, we demonstrated that CFTRoverexpression could reverse the decreased PI3K/AKT expression induced by the I/R treatment invivo or H/R treatment in *** results of the present study indicate that the overexpression of CFTR protects Caco2 cells fromH/R-induced apoptosis;furthermore, it also inhibits H/R-induced apoptosis through thePI3K/AKT/NF-κB signaling pathway in H/R-treated Caco2 cells and intestinal tissues.

关键词： Apoptosis cystic fibrosis transmembrane conductance regulator Intestinal ischemia-reperfusion injury PI3K/AKT/NF-κB Hypoxia/reoxygenation Caco2 cells

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Expression of cystic fibrosis transmembrane conductance regulator in Rat Ovary

引用

Journal of Huazhong University of Science and Technology(Medical Sciences) 2008年第5期28卷 584-587页

作者：靳镭汤瑞玲 Department of Obstetrics and Gynecology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology

The protein expression of cystic fibrosis transmembrane conductance regulator （CFTR）, a cAMP-activated Cl- channel, in ovarian stimulated premature female rat ovary during a cycle of follicle development and corpus luteum formation was investigated. Animals were injected with 10 U pregnant Mare＇s serum gonadotropin （PMSG） and subsequently 10 U hCG 48 h later. Time-dependent immunohistochemistry and Western blotting experiments were performed before and 24, 48, 72 h after hCG treatment. The immunohistochemistry revealed that administration of PMSG stimulated the CFTR expression in thecal cell layer and granulosa cell layer of mature follicles 48 h post injection, coincident with the PMSG-induced peak in follicular estradiol. However, the expression of CFTR in the granulose lutein cell layer and thecal lutein cell layer was time-dependently reduced following hCG injection, in accordance with the gradually increased progestogen level during luteum corpus formation. Western blotting analysis demonstrated that rat ovarian tissue expressed the special CFTR band at 170 kD. It is concluded that cAMP-dependent Cl- channels are involved in regulation of follicle development and luteum formation.

关键词： cystic fibrosis transmembrane conductance regulator Cl- channel rat ovary follicle corpus luteum

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Identification of risk genes in Chinese nonobstructive azoospermia patients based on whole-exome sequencing

引用

Asian Journal of Andrology 2023年第1期25卷 66-72页

作者： Yu-Jun Liu Xin-Jie Zhuang Jian-Ting An Hui Jiang Rong Li Jie Qiao Li-Ying Yan Xu Zhi Center for Reproductive Medicine Department of Obstetrics and GynecologyPeking University Third HospitalBeijing 100191China National Clinical Research Center for Obstetrics and Gynecology Beijing 100191China Key Laboratory of Assisted Reproduction Peking UniversityMinistry of EducationBeijing 100191China Peking-Tsinghua Center for Life Sciences Peking UniversityBeijing 100191China Department of Urology Peking University Third HospitalBeijing 100191China Department of Andrology Peking University Third HospitalBeijing 100191China

Nonobstructive azoospermia(NOA)is a severe condition in infertile men,and increasing numbers of causative genes have been identified during the last few *** certain causative genes can explain the presence of NOA in some patients,a proportion of NOA patients remain to be *** study aimed to investigate potential high-risk genes associated with spermatogenesis in idiopathic NOA patients by whole-exome ***-exome sequencing was performed in 46 male patients diagnosed with ***,screening was performed for 119 genes known to be related to male ***,further screening was performed to determine potential high-risk causative genes for NOA by comparisons with 68 healthy male ***,risk genes with high/specific expression in the testes were selected and their expression fluctuations during spermatogenesis were *** frequency of cystic fibrosis transmembrane conductance regulator(CFTR)gene pathogenic variant carriers was higher in the NOA patients compared with the healthy *** risk genes that may be causes of NOA were identified,including seven genes that were highly/specifically expressed in the *** risk genes previously reported to be involved in spermatogenesis(MutS homolog 5[MSH5],cilia-and flagella-associated protein 54[CFAP54],MAP7 domain containing 3[MAP7D3],and coiled-coil domain containing 33[CCDC33])and three novel risk genes(coiled-coil domain containing 168[CCDC168],chromosome 16 open reading frame 96[C16orf96],and serine protease 48[PRSS48])were identified to be highly or specifically expressed in the testes and significantly different in the 46 NOA patients compared with 68 healthy *** study on clinical NOA patients provides further evidence for the four previously reported risk *** present findings pave the way for further functional investigations and provide candidate risk genes for genetic diagnosis of NOA.

关键词： cystic fibrosis transmembrane conductance regulator nonobstructive azoospermia potential risk genes spermatogenesis whole-exome sequencing

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

CFTR mutations causing congenital unilateral absence of the vas deferens(CUAVD)and congenital absence of the uterus(CAU)in a consanguineous family

引用

Asian Journal of Andrology 2022年第4期24卷 416-421页

作者： Mahdieh Daliri Ghouchanatigh Ranjha Khan Majid Mojarrad Uzma Hameed Muhammad Zubair Ahmed Waqas Mohsen Jalali Mahmoudreza Kalantari Ali Shamsa Huan Zhang Qing-Hua Shi The First Affiliated Hospital of USTC Hefei National Laboratory for Physical Sciences at the MicroscaleSchool of Life SciencesCAS Center for Excellence in Molecular Cell ScienceUniversity of Science and Technology of ChinaCollaborative Innovation Center of Genetics and DevelopmentHefei 230027China Department of Medical Genetics School of MedicineMashhad University of Medical SciencesMashhad 91735Iran Institute of Industrial Biotechnology Government College UniversityLahore 54000Pakistan Department of Zoology Division of Science and TechnologyUniversity of Education LahoreMultan CampusMultan 60000Pakistan Department of Reproductive Medicine Armaghan Infertility CenterMashhad 91735Iran Department of Pathology Mashhad University of Medical SciencesMashhad 91735Iran.

cystic fibrosis(CF)is one of the most common recessive genetic diseases,with a wide spectrum of phenotypes,ranging from infertility to severe pulmonary *** in the cystic fibrosis transmembrane conductance regulator(CFTR)gene are considered the main genetic cause for *** this study,we recruited a consanguineous Iranian pedigree with four male patients diagnosed with congenital unilateral absence of the vas deferens(CUAVD),and one female patient diagnosed with congenital absence of the uterus(CAU).Testicular biopsy of one patient was performed,and hematoxylin and eosin(H and E)staining of testis sections displayed the presence of germ cell types ranging from spermatogonia to mature spermatids,indicating obstructive *** explore the underlying genetic factor in this familial disorder,we therefore performed whole-exome sequencing(WES)on all available family *** data filtration and CFTR haplotype analysis identified compound heterozygous mutations in CFTR among four patients(two CUAVD patients carried p.H949Y and p.L997F,and one CUAVD and the female CAU patient carried p.H949Y and p.I148T).All these mutations were predicted to be deleterious by at least half of the prediction software programs and were confirmed by Sanger *** study reported that CFTR compound heterozygous mutations in a consanguineous Iranian family cause infertility in both sexes.

关键词： congenital absence of the uterus congenital unilateral absence of the vas deferens cystic fibrosis transmembrane conductance regulator whole-exome sequencing

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Epithelial Sodium and Chloride Channels and Asthma

引用

Chinese Medical Journal 2015年第16期128卷 2242-2249页

作者： Wen Wang, Hong-Long Ji Department of Respiratory and Critical Care Medicine Beijing Chaoyang Hospital Capital Medical University Beijing Institute of Respiratory Medicine Beijing Key Laboratory of Respiratory and Pulmonary Circulation Disorders Beijing 100020 China Department of Cellular and Molecular Biology Texas Lung Injury Institute University of Texas Health Science Center at Tyler Tyler Texas 75708 USA

Objective：To focus on the asthmatic pathogenesis and clinical manifestations related to epithelial sodium channel （ENaC）/chlorine ion *** Sources：The data analyzed in this review were the English articles from 1980 to 2015 from journal databases,primarily PubMed and Google *** terms used in the literature search were：（1） ENaCs;cystic fibrosis （CF） transmembrane conductance regulator （CFTR）;asthma/asthmatic,（2） ENaC/sodium salt;CF;asthma/asthmatic,（3） CFTR/chlorine ion channels;asthma/asthmatic,（4） ENaC/sodium channel/scnn1a/scnn1b/scnn1g/scnn1d/amiloride-sensitive/amiloride-inhibtable sodium channels/sodium salt;asthma/asthmatic,lung/pulmonary/respiratory/tracheal/alveolar,and （5） CFTR;CF;asthma/asthmatic （ti）.Study Selection：These studies included randomized controlled trials or studies covering asthma pathogenesis and clinical manifestations related to ENaC/chlorine ion channels within the last 25 years （from 1990 to 2015）.The data involving chronic obstructive pulmonary disease and CF obtained from individual studies were also reviewed by the ***：Airway surface liquid dehydration can cause airway inflammation and *** and CFTR are closely related to the airway mucociliary *** transporters may play a critical role in pathogenesis of asthmatic ***：Ion channels have been the center of many studies aiming to understand asthmatic pathophysiological mechanisms or to identify therapeutic targets for better control of the disease.

关键词： Airway Surface Liquid Asthma cystic fibrosis transmembrane conductance regulator Epithelial Sodium Channel Mucociliary Clearance

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Identification of the second CFTR mutation in patients with congenital bilateral absence of vas deferens undergoing ART protocols

引用

Asian Journal of Andrology 2010年第6期12卷 819-826页

作者： Rossella.Giuliani Ivana Antonucci Isabella Torrente Paola Grammatico Giandomenico Palka Liborio Stuppia Department of Clinical Sciences and Bioimages ＇G. d＇Annunzio ＇University Chieti-Pescara 66013 Italy Department of Biomedical Sciences ＇G. d＇Annunzio＇ University Chieti-Pescara 66013 Italy IRCCS CSS-Mendel Institute Rome 00198 Italy Medical Genetics Laboratory Experimental Medicine Department Sapienza S. Camillo-Forlanini Hospital Universityof Rome Rome 00152 Italy Human Genetic Division Pescara Hospital Pescara 65100 Italy I.G.M. CNR c/o IOR Bologna 40136 Italy

Congenital bilateral absence of vas deferens （CBAVD） is a manifestation of the mildest form of cystic fibrosis （CF） and is characterized by obstructive azoospermia in otherwise healthy patients. Owing to the availability of assisted reproductive technology, CBAVD patients can father children. These fathers are at risk of transmitting a mutated allele of the CF transmembrane conductance regulator （CFTR） gene, responsible for CF, to their offspring. The identification of mutations in both CFTR alleles in CBAVD patients is a crucial requirement for calculating the risk of producing a child with full-blown CF if the female partner is a healthy CF carrier. However, in the majority of CBAVD patients, conventional mutation screening is not able to detect mutations in both CFTR alleles, and this difficulty hampers the execution of correct genetic counselling. To obtain information about the most represented CFTR mutations in CBAVD patients, we analysed 23 CBAVD patients, 15 of whom had a single CFTR mutation after screening for 36 mutations and the 5T allele. The search for the second CFTR mutation in these cases was performed by using a triplex approach：（i） first, a reverse dot-blot analysis was performed to detect mutations with regional impact; （ii） next, multiple ligation-dependent probe amplification assays were conducted to search for large rearrangements; and （iii） finally, denaturing high-performance liquid chromatography was used to search for point mutations in the entire coding region. Using these approaches, the second CFTR mutation was detected in six patients, which increased the final detection rate to 60.8%.

关键词： congenital bilateral absence of vas deferens cystic fibrosis transmembrane conductance regulator male infertility

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Interference with the formation of the epididymal microenvironment——a new strategy for male contraception?

引用

Asian Journal of Andrology 2000年第1期2卷 39-45页

作者： X.D.Gong G.P.H.Leung B.L.Y.Cheuk P.Y.D.Wong Department of Phsiology The Chinese University of Hong Kong Shatin N. T. Hong Kong ChinaDepartment of Phsiology The Chinese University of Hong Kong Shatin N. T. Hong Kong ChinaDepartment of Phsiology The Chinese University of Hong Kong Shatin N. T. Hong Kong ChinaDepartment of Phsiology The Chinese University of Hong Kong Shatin N. T. Hong Kong China

The cystic fibrosis transmembrane conductance regulator (CFIR) or the small conductance cAMP-activated chlo-ride channel encoded by the CFTR gene has been shown to play an important role in the formation of the epididymalfluid microenvironment. Recent work in our laboratory has shown that this protein is also expressed by developing germcells indicating a role of this protein in spermatogenesis. In view of the fact that the CFTR gene has a far reaching andwidespread effect on human reproduction, understanding the role of CFTR in the male reproductive tissues and its inter-vention by pharmacological agents can open a new avenue of research into the development of novel male contracep-tives. (Asian J Androl 2000; 2: 39 - 45 )

关键词： epididymis cyclooxygenase contraception cystic fibrosis transmembrane conductance regulator

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Ginkgolide C Stimulates CFTR-mediate Anion conductance in Distal Colon:Implication for Therapy of Gastrointestinal Diseases

引用

Chemical Research in Chinese Universities 2009年第6期25卷 909-913页

作者： LIAO Qi MA Xiao-yan WU Fu-ju YANG Li-xiao WANG Shuai Second Teaching Hospital of Jilin University Changchun 130041 P. R. China The Daqing Longnan Hospital Daqing 163453 P. R. China

The effects and the mechanisms of natural compounds ginkgolides on CFTR-mediate anion transport were investigated. The CFTR-mediate iodide influx rates were studied via a cell-based fluorescence assay done for FRT cells stably transfected by CFTR; transepithelial short-circuit current recordings of FRT cells and rat distal colon mucosa were respectively obtained. Cellular cAMP concentrations were measured via a radioimmunoassay analysis kit. Ginkgolide C dose-dependently increases CFTR-mediate anion transport, whereas ginkgolide A and B show no effect. The activation is sensitive to CFTR specific activator CFTRinh-172. Ginkgolide C stimulated amiloride and indomethacin pre-treated Cl currents in rat distal colon mucosa. Studies on FRT cells also manifest that ginkgolide C had additive effect with FSK/IBMX mixture and didn＇t elevate intracellular cAMP concentration, which implies it works through a direct binding mechanism. In conclusion, Ginkgolide C directly stimulates CFTR-mediate anion transport. Ginkgolide C may be a promising drug for the prevention and treatment of CFTR-related diseases such as idiopathic chronic pancreatitis（ICP）, habitual constipation, and kcratoconjunctivitis sicca（KCS）.

关键词： Ginkgolide cystic fibrosis transmembrane conductance regulator Fluorescence Mucosa

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Direct Activation of CFTR Chloride Channel by Natural Compound Theophylline

引用

Chemical Research in Chinese Universities 2009年第6期25卷 887-890页

作者： LIU Xin GE Hong ZHAO Xue-liang HOU Ting-ting SUN Juan-juan YANG Jing-bo HE Cheng-yan YANG Hong China-Japan Union Hospital of Jilin University Changchun 130033 P. R. China School of Life Sciences Liaoning Provincial Key Laboratory of Biotechnology and Drug Discovery Liaoning Normal University Dalian 116029 P. R. China School of Chemical Engineering and Technology Tianjin University Tianjin 300072 P. R. China

Theophylline has been widely used in the＇treatment of airway disease but molecular mechanism has not been clearly elucidated. Previous studies have manifested that theophylline increases intracellular cAMP concentra- tion. Because cystic fibrosis transmembrane conductance regulator（CFTR） is a cAMP-dependent Cl- channel that plays key roles in fluid secretion in vivo, we postulated that theophylline activates CFTR channel gating. We found （1） theophylline stimulated CFTR-mediated anion transport in a concentration-dependent manner, and CFTR specific blocker completely reversed the effect; （2） theophylline had no effect on △F508 or G551D mutant CFTR chloride channel activity; （3） theophylline had additive effect with forskolin（FSK） and 3-isobutyl-xanthin（IBMX）, thus a direct binding activation mechanism was suggested. In conclusion, the results may provide a clue to elucidating the molecular mechanism of theophylline activities and theophylline may present a novel lead drug in treating CFTR-related disease.

关键词： Theophylline cystic fibrosis transmembrane conductance regulator Activator

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

cystic fibrosis-related diabetes:The unmet need

引用

World Journal of Diabetes 2020年第6期11卷 213-217页

作者： Leonardo Pozo Fatimah Bello Yamely Mendez Salim Surani Internal Medicine University of Texas Rio Grande ValleyEdinburgTX 78539United States Research Department Baylor College of MedicineHoustonTexas.77030United States Medical Critical Care Services Corpus Christi Medical CenterCorpus ChristiTX 78404United States

cystic fibrosis(CF)is a common autosomal recessive *** expectancy of patients with CF continues to improve mainly driven by the evolving therapies for CF-related organ *** prevalence of CF-related diabetes(CFRD)increases exponentially as patients’*** care guidelines for CFRD from 2010,recommend insulin as the mainstay of *** patients with CFRD may not require exogenous insulin due to the heterogeneity of this clinical *** of euglycemia by enhancing endogenous insulin production,secretion and degradation with novel pharmacological therapies like glucagonlike peptide-1 agonist is an option that remains to be fully *** such,the scope of this article will focus on our perspective of glucagon-like peptide-1 receptor agonist in the context of *** potential options such as sodiumglucose cotransporter-2 and dipeptidyl peptidase 4 inhibitors and their impact on this patient population is limited and further studies are required.

关键词： cystic fibrosis cystic fibrosis-related diabetes cystic fibrosis transmembrane conductance regulator Gastric inhibitory polypeptide Glucagon-like peptide 1 Glucagon-like peptide-1 receptor agonist Dipeptidyl peptidase 4 inhibitors

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：