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cyclin gene SlCycB1 alters plant architecture in association with histone H3.2 in tomato

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Horticultural Plant Journal 2022年第3期8卷 341-350页

作者： Tingyan Zhang Yaru Wang Shoaib Munir Taotao Wang Zhibiao Ye Junhong Zhang Yuyang Zhang Key Laboratory of Horticultural Plant Biology(Ministry of Education) College of Horticulture and Forestry ScienceHuazhong Agricultural UniversityWuhan 430070China Hubei Hongshan Laboratry Wuhan 430070China

Cell cycle regulation plays a critical role in plant growth and *** this study,the role of a tomato cell cycle gene SlCycB1 has been *** analysis revealed that SlCycB1 was mostly expressed in stem,root,and leaves,with relative lower expression in flower and *** plants overexpressing SlCycB1 exhibited a reduction in cell number and increased cell size leading to the growth ***,yeast two-hybrid analysis and bimolecular fluorescence complementation revealed that SlCycB1 interacted with histone H3.2,an essential component of the *** H3.2 was transcriptionally up-regulated in the SlCycB1 overexpressing tomato ***,the overexpression of histone H3.2 in transgenic plants showed similar phenotypes to SlCycB1 overexpressing *** on these findings,we concluded that SlCycB1 overexpression altered tomato architecture in association with histone H3.2.

关键词： Tomato cyclin SlCycB1 Histone Growth and development

cyclin Cyc2p is required for micronuclear bouquet formation in Tetrahymena thermophila

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Science China(Life Sciences) 2019年第5期62卷 668-680页

作者： Jing Xu Xiaoxiong Li Weibo Song Wei Wang Shan Gao Institute of Evolution & Marine Biodiversity Ocean University of China College of Life Science Shanxi University Key Laboratory of Chemical Biology and Molecular Engineering of the Ministry of Education Institute of Biotechnology Shanxi University Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and Technology College of Marine Life Sciences Ocean University of China

Meiotic bouquet formation(known as crescent formation in Tetrahymena thermophila) is indispensable for homologous pairing and recombination, but the regulatory mechanism of bouquet formation remains largely unknown. As a conjugation specific cyclin gene, CYC2 knockout mutants failed to form an elongated crescent structure and aborted meiosis progress in T. thermophila. γ-H2 A.X staining revealed fewer micronuclear DNA double-strand breaks(DSBs) in cyc2Δ cells than in wild-type cells. Furthermore, cyc2Δ cells still failed to form a crescent structure even though DSBs were induced by exogenous agents,indicating that a lack of DSBs was not completely responsible for failure to enter the crescent stage. Tubulin staining showed that impaired perinuclear microtubule structure may contribute to the blockage in micronuclear elongation. At the same time,expression of microtubule-associated kinesin genes, KIN11 and KIN141, was significantly downregulated in cyc2Δ ***, micronuclear specific accumulation of heterochromatin marker trimethylated H3 K23 abnormally increased in the cyc2Δ mutants. Together, these results show that cyclin Cyc2 p is required for micronuclear bouquet formation via controlling microtubule-directed nuclear elongation in Tetrahymena.

关键词： Tetrahymena meiosis cyclin Cyc2p bouquet formation

2,3,7,8-Tetrachlorodibenzo-p-dioxin Promotes Proliferation of Astrocyte Cells via the Akt/STAT3/cyclin D1 Pathway

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Biomedical and Environmental Sciences 2019年第4期32卷 281-290页

作者： WU Chang Yue YIN Kai Zhi ZHANG Yan JIAO Man ZHAO Xin Yuan WU Qi Yun Medical Colledge Nantong University Department of Nutrition and Food Hygiene School of Public HealthNantong University Xishan District Center for Disease Control and Preventive Department of Occupational Medicine and Environmental Toxicology School of Public HealthNantong University

Objective The compound 2,3,7,8-tetrachlorodibenzo-p-dioxin(TCDD), a persistent organic pollutant, is harmful to the nervous system, but its effects on the brain are still unclear. This study aimed to investigate the effects of TCDD on astrocytes proliferation and underlying molecular mechanism. Methods The cell proliferation was measured by EdU-based proliferation assay and PI staining by flow cytometry. Protein expression levels were detected by Western blotting. Immunofluorescence, cytoplasmic and nuclear fractions separation were used to assess the distribution of signal transducer and activator of transcription 3(STAT3). Results C6 cells treated with 10 and 50 nmol/L TCDD for 24 h showed significant promotion of the proliferation of. The exposure to TCDD resulted in the upregulation in the expression levels of phosphorylated protein kinase B(p-Akt), phosphorylated STAT3, and cyclin D1 in a dose-and time-dependent manner. The inhibition of Akt expression with LY294002 or STAT3 expression with AG490 abolished the TCDD-induced cyclin D1 upregulation and cell proliferation. Furthermore, LY294002 suppressed the activation of STAT3. Finally, TCDD promoted the translocation of STAT3 from the cytoplasm to the nucleus, and LY294002 treatment blocked this effect. Conclusion TCDD exposure promotes the proliferation of astrocyte cells via the Akt/STAT3/cyclin D1 pathway, leading to astrogliosis.

关键词： 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) Akt STAT3 cyclin D1 Proliferation Astrocytes

Brucea javanica oil emulsion improves the effect of radiotherapy on esophageal cancer cells by inhibiting cyclin D1-CDK4/6 axis

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World Journal of Gastroenterology 2019年第20期25卷 2463-2472页

作者： Zhong-Hua Qiu Wei-Wei Zhang Hong-Hua Zhang Gui-Hua Jiao Department of Neurology Liangshan County People's HospitalJining272600Shandong ProvinceChina Department of Neurology Liangshan County People's HospitalJining272600Shandong ProvinceChina

BACKGROUND Esophageal cancer is one of the most common cancers around the world, and it has high incidence and mortality rates. The conventional therapy for esophageal cancer is radiotherapy, although its effect is highly limited by the resistance of esophageal cancer cells. Thus, strong radiosensitizers can be very crucial during radiotherapy against esophageal cancer. Brucea javanica oil emulsion (BJOE) is a widely used drug against various cancers, such as liver, colon, and ovarian cancer. However, its anti-cancer effect and mechanism and the use of BJOE as a radiosensitizer have not been explored in esophageal cancer. AIM To evaluate the anti-cancer effect and mechanism of BJOE and explore the potential use of BJOE as a radiosensitizer during radiotherapy. METHODS The inhibitory effect of BJOE and its enhancement function with radiation on cell viability were examined with the calculated half-maximal effective concentration and half-maximal lethal concentration. The influence of BJOE on cell migration and invasion were measured with EC109 and JAR cells by wound-healing and transwell assay. Clonogenesis and apoptotic rate, which was measured by Hoechst staining, were investigated to confirm its enhancement function with radiation. To investigate the molecular pathway underlying the effect of BJOE, the expressions of several apoptosis- and cycle-related proteins was detected by western *** lines more than normal cell lines, and it markedly reduced migration and invasion in esophageal cancer cells (EC109 and JAR). Moreover, it promoted cell apoptosis and enhanced the effect of radiotherapy against esophageal cancerous cells. In the viability test, the values of half-maximal effective concentration and half-maximal lethal concentration were reduced. Compared to the control, only around 1/5 colonies formed when using BJOE and radiation together in the clonogenic assay. The apoptotic rate in EC109 was obviously promoted when BJOE was added during radiotherap

关键词： Esophageal cancer Brucea javanica oil emulsion Radiosensitizer Apoptosis cyclin D1-CDK4/6 axis

Effect of chitooligosaccharides on cyclin D1,bcl-xl and bcl-2 mRNA expression in A549 cells using quantitative PCR

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Chinese Science Bulletin 2011年第15期56卷 1629-1632页

作者： LI Xian WANG Ju CHEN XiaoJia TIAN JinHuan LI LiHua ZHAO MingYan JIAO YanPeng ZHOU ChangRen Department of Materials Science and Engineering Jinan University Guangzhou 510632 China1 Bioengineering Institute Jinan University Guangzhou 510632 China

Chitooligosaccharide (COS) is derived from the chemical and enzymatic hydrolysis of chitosan and has been reported to have potent antitumor *** study investigated the effects of five chitooligomers ranging from the dimer form to the hexamer form (chitobiose,chitotriose,chitotetraose,chitopentaose,chitohexaose) on the expression of cyclin D1,bcl-2 and bcl-xl mRNA in A549 cells using reverse transcription quantitative real-time *** demonstrated that,of the five chitooligomers used,chitohexaose (COS6) had the most potent inhibitory effect on A549 cell ***6 also significantly down regulated cyclin D1 and bcl-xl mRNA expression *** data suggested that COS6 exerts its antitumor activity by two different mechanisms:(1) COS6-mediated inhibition of cyclin D1 levels leads to suppression of tumor cell proliferation;and (2) COS6-mediated down-regulation of the pro-survival protein,Bcl-xl,promotes the apoptosis of tumor cells.

关键词：细胞周期蛋白D1 mRNA表达水平 A549细胞壳寡糖定量PCR 肿瘤细胞凋亡抗肿瘤活性 cyclin

Epstein-Barr virus-encoded latent membrane protein 1 modulates cyclin D1 by c-Jun/Jun B heterodimers

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Science China(Life Sciences) 2005年第4期48卷 385-393页

作者： SONG Xin1, TAO Yongguang1, ZENG Liang1, YANG Jing1, TANG Faqing1, Leo M. Lee2, GONG Jianping3, WU Qiao4 & CAO Ya1 1. Cancer Research Institute, Xiangya School of Medicine, Central South University, Changsha 410078, China 2. Laboratory of Molecular Technology SAIC-Frederick, National Cancer Institute P.O. Box B, Frederick, MD 21702, USA 3. Molecular Medical Center, Tongji Hospital, Tongji Medical University, Wuhan 430030, China 4. Key Laboratory of the Ministry of Education for Cell Biology, and Tumor Cell Engineering, School of Life Sciences, Xiamen University, Xiamen 361005, China 1. Cancer Research Institute Xiangya School of Medicine Central South University 410078 Changsha China 2. Laboratory of Molecular Technology SAIC-Frederick National Cancer Institute P.O. Box B 21702 Frederick MD USA 3. Molecular Medical Center Tongji Hospital Tongji Medical University 430030 Wuhan China 4. Key Laboratory of the Ministry of Education for Cell Biology and Tumor Cell Engineering School of Life Sciences Xiamen University 361005 Xiamen China

In our recent studies, we found that LMP1 encoded by Epstein-Barr virus could accelerate the formation of active c-Jun/Jun B heterodimer. We studied the regulation of cyclinD1 by c-Jun/Jun B heterodimers by laser scanning confocal influorescence microscopy, Western blot, luciferase activity assay, super-EMSA and flow cytometry in the Tet-on-LMP1 HNE2 cell line, in which LMP1 expression was regulated by Tet-on system. c-Jun/Jun B heterodimers induced by LMP1 could up regulate cyclin D1 promoter activity and expression. Overexpression of cy-clinD1 accelerated the progression of cell cycle.

关键词： latent membrane protein1, c-Jun, Jun B, heterodimer, cyclin D1, cell cycle.

RNA干扰cyclin D1基因表达对瘢痕疙瘩成纤维细胞增殖和G1期调控的影响(英文)

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生物化学与生物物理进展 2008年第2期35卷 159-169页

作者：于冬梅郝立君王冬艳王慧冬哈尔滨医科大学附属第二院整形美容科哈尔滨150086 哈尔滨医科大学附属第四医院哈尔滨150007

为研究siRNA干扰瘢痕疙瘩成纤维细胞cyclin D1基因表达,对瘢痕疙瘩成纤维细胞的增殖、细胞周期和G1期调控的影响,构建了靶向cyclin D1的siRNA表达质粒.利用LipofectamineTM2000转染体外培养的瘢痕疙瘩成纤维细胞,应用荧光定量PCR、RT-PC... 详细信息

为研究siRNA干扰瘢痕疙瘩成纤维细胞cyclin D1基因表达,对瘢痕疙瘩成纤维细胞的增殖、细胞周期和G1期调控的影响,构建了靶向cyclin D1的siRNA表达质粒.利用LipofectamineTM2000转染体外培养的瘢痕疙瘩成纤维细胞,应用荧光定量PCR、RT-PCR检测cyclin D1 mRNA的干扰效果,应用MTT法、流式细胞仪检测细胞增殖和细胞周期的变化,应用免疫组织化学染色检测成纤维细胞中cyclin D1、CDK4、P16、pRb蛋白表达的影响.主要结果如下:a.靶向cyclin D1的特异性siRNA序列可以高效地抑制成纤维细胞cyclin D1基因表达,对照组与实验组在mRNA水平其表达抑制率分别为63.68%和92.83%(Pcyclin D1、CDK4和pRb蛋白,在瘢痕疙瘩成纤维细胞中均出现了不同程度的表达下调,而低表达的P16则呈上调表现.由上述结果可见,构建的靶向cyclin D1的RNAi表达质粒,可有效地抑制瘢痕疙瘩成纤维细胞cyclin D1基因表达,通过改变G1期相关周期蛋白的水平,影响G1/S期的进程,显著地抑制成纤维细胞的增殖.

关键词：瘢痕疙瘩 RNA干扰 cyclin D1 CDK4 pRb P16 细胞周期

cyclin G1在小鼠卵巢的表达及其与卵泡发育的关系

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四川大学学报（医学版） 2006年第6期37卷 893-897页

作者：柳朝华张惠何亚平张金虎岳利民四川大学华西基础医学与法医学院生理学教研室成都610041

目的观察细胞周期素G1(cyclinG1)在小鼠卵巢中各级卵泡的表达及其与卵泡发育的关系。方法性成熟前小鼠用孕马血清促性腺激素(PMSG)刺激卵泡生长,用绒毛膜促性腺激素(hCG)刺激卵泡排卵及向黄体转化。于给药后不同时间取出卵巢,用免疫组... 详细信息

目的观察细胞周期素G1(cyclinG1)在小鼠卵巢中各级卵泡的表达及其与卵泡发育的关系。方法性成熟前小鼠用孕马血清促性腺激素(PMSG)刺激卵泡生长,用绒毛膜促性腺激素(hCG)刺激卵泡排卵及向黄体转化。于给药后不同时间取出卵巢,用免疫组化方法检测不同发育阶段卵泡cyclinG1蛋白的表达和分布;用细胞免疫荧光方法检测不同成熟阶段的卵细胞中cyclinG1蛋白表达。结果免疫组化染色结果显示,颗粒细胞中Cy-clinG1的表达水平随卵泡发育成熟而逐渐增强,且一直定位在胞核;cyclinG1在各个时期卵泡的卵母细胞中均有表达,该表达的定位与卵泡生长发育的不同阶段有关,随着卵泡的发育,cyclinG1在卵母细胞的表达由胞核转至胞浆。至排卵前卵泡颗粒细胞,cyclinG1表达水平减弱,当颗粒细胞分化为黄体细胞后表达水平进一步减弱。闭锁卵泡中cyclinG1的表达水平很弱。细胞免疫荧光染色结果显示,处于生发泡时期(GV)卵母细胞仅有较弱的cyclinG1表达,当卵母细胞生发泡破裂(GVBD)后,该表达增强;与处于第二次减数分裂中期(M)的卵母细胞相比,受精卵的cyclinG1表达明显增强。结论cyclinG1在小鼠卵泡发育及卵母细胞成熟过程中的表达具有时空特异性,可能作为细胞周期的正调节因子参与了卵泡生长发育和卵母细胞成熟过程的调控。

关键词： cyclin G1卵泡卵母细胞小鼠

cyclin D1调控肺癌细胞的浸润和转移

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生理学报 2012年第1期64卷 55-61页

作者：李尊岭邵淑红焦飞岳真马颖滨州医学院生物化学与分子生物学教研室分子遗传学研究所山东省教育厅肿瘤分子生物学重点实验室烟台264003 滨州医学院基础心理学教研室烟台264003

cyclin D1作为细胞周期的一个调控因子,许多肿瘤细胞都过表达cyclin D1基因,比如肺癌、乳腺癌、甲状腺癌等。但是cyclin D1在肿瘤细胞浸润和转移中的作用报道较少。本研究旨在研究cyclin D1对肺癌细胞浸润、转移的调控作用。以肺腺癌细... 详细信息

cyclin D1作为细胞周期的一个调控因子,许多肿瘤细胞都过表达cyclin D1基因,比如肺癌、乳腺癌、甲状腺癌等。但是cyclin D1在肿瘤细胞浸润和转移中的作用报道较少。本研究旨在研究cyclin D1对肺癌细胞浸润、转移的调控作用。以肺腺癌细胞A549(高表达cyclin D1)和肺鳞癌细胞SK-MES-1(低表达cyclin D1)为研究对象。取裸鼠尾静脉注射A549或SK-MES-1细胞,4周后处死,从肺脏中分离出肿瘤细胞再接种另外的裸鼠,如此经过2次循环接种构建裸鼠高转移动物模型。向A549细胞转染cyclin D1的干扰RNA抑制cyclin D1的表达,同时向SK-MES-1细胞转染cyclin D1的表达载体促进cyclin D1的表达,随后通过细胞迁移实验观察基因处理后A549和SK-MES-1细胞的转移能力变化。用Western blot检测亲本细胞、不同转移层次裸鼠的癌组织中cyclin D1和WNT/TCF信号通路相关蛋白因子的表达。结果显示,随着转移次数增加,从动物癌组织中分离出的癌细胞转移能力显著增强,cyclin D1表达也明显升高;在A549细胞中,干扰cyclin D1的表达可使细胞迁移能力变弱;在SK-MES-1细胞中,过表达cyclin D1可使细胞迁移能力增强。高转移肺癌动物模型肿瘤组织中β-catenin、LEF、TCF蛋白表达高于低转移肿瘤组织,而二者的上述蛋白表达均高于亲本肺癌细胞。以上结果提示,cyclin D1的表达与肺癌的浸润和转移密切相关,推测WNT/TCF信号通路能够促进cyclin D1的表达。

关键词：肺肿瘤 cyclin D1 干扰RNA 肿瘤转移 WNT信号通路