Effect of chitooligosaccharides on cyclin D1,bcl-xl and bcl-2 mRNA expression in A549 cells using quantitative PCR

作     者：LI Xian WANG Ju CHEN XiaoJia TIAN JinHuan LI LiHua ZHAO MingYan JIAO YanPeng ZHOU ChangRen 

作者机构：Department of Materials Science and Engineering Jinan University Guangzhou 510632 China1 Bioengineering Institute Jinan University Guangzhou 510632 China 

出 版 物：《Chinese Science Bulletin》 (Chin. Sci. Bull.)

年 卷 期：2011年第56卷第15期

页      面：1629-1632页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 

基　　金：supported by the National High Technology Research and Development Program of China (2007AA091603) 

主　　题：细胞周期蛋白D1 mRNA表达水平 A549细胞 壳寡糖 定量PCR 肿瘤细胞凋亡 抗肿瘤活性 cyclin 

摘      要：Chitooligosaccharide (COS) is derived from the chemical and enzymatic hydrolysis of chitosan and has been reported to have potent antitumor *** study investigated the effects of five chitooligomers ranging from the dimer form to the hexamer form (chitobiose,chitotriose,chitotetraose,chitopentaose,chitohexaose) on the expression of cyclin D1,bcl-2 and bcl-xl mRNA in A549 cells using reverse transcription quantitative real-time *** demonstrated that,of the five chitooligomers used,chitohexaose (COS6) had the most potent inhibitory effect on A549 cell ***6 also significantly down regulated cyclin D1 and bcl-xl mRNA expression *** data suggested that COS6 exerts its antitumor activity by two different mechanisms:(1) COS6-mediated inhibition of Cyclin D1 levels leads to suppression of tumor cell proliferation;and (2) COS6-mediated down-regulation of the pro-survival protein,Bcl-xl,promotes the apoptosis of tumor cells.