检索结果-南通市图书馆

The occurrence order and Cross-talk of different tRNA modifications

在线全文

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2021年第9期64卷 1423-1436页

作者： Jing Li Wen-Yu Zhu Wen-Qing Yang Cai-Tao Li Ru-Juan Liu School of Life Science and Technology ShanghaiTech UniversityShanghai 201210China

Chemical modifications expand the composition of RNA molecules from four standard nucleosides to over 160 modified nucleosides,which greatly increase the complexity and utility of RNAs.Transfer RNAs(tRNAs)are the most heavily modified cellular RNA molecules and contain the largest variety of modifications.Modification of tRNAs is pivotal for protein synthesis and also precisely regulates the noncanonical functions of tRNAs.Defects in tRNA modifications lead to numerous human diseases.Up to now,more than 100 types of modifications have been found in tRNAs.Intriguingly,some modifications occur widely on all tRNAs,while others only occur on a subgroup of tRNAs or even only a specific tRNA.The modification frequency of each tRNA is approximately 7% to 25%,with 5-20 modification sites present on each tRNA.The occurrence and modulation of tRNA modifications are specifically noticeable as plenty of interplays among different sites and modifications have been discovered.In particular,tRNA modifications are responsive to environmental changes,indicating their dynamic and highly organized nature.In this review,we summarized the known occurrence order,Cross-talk,and cooperativity of tRNA modifications.

关键词： tRNA RNA modification RNA methylation occurrence order Cross-talk

基于肠源性高密度脂蛋白调控脂多糖介导Kupffer-肝细胞Cross-talk探讨脾虚膏脂转输障碍的分子生物学基础

在线全文

学校读者我要写书评

暂无评论

中华中医药学刊 2023年第2期41卷 75-77页

作者：翟亚荣孟嘉伟张琦裘雪莹于宁王杰曹慧敏王群隋国媛贾连群南方医科大学中西医结合医院广东广州510315 辽宁中医药大学中医脏象理论及应用教育部重点实验室辽宁沈阳110847

脾虚膏脂转输障碍是血脂异常的关键病机。脾失健运引起肝脏代谢功能障碍可能导致血脂异常。肠道功能紊乱是脾虚膏脂转输障碍的启动因素,以其介导“炎症-代谢”紊乱为切入点,深入揭示血脂异常疾病发病机制以提高中西医结合防治水平逐渐... 详细信息

脾虚膏脂转输障碍是血脂异常的关键病机。脾失健运引起肝脏代谢功能障碍可能导致血脂异常。肠道功能紊乱是脾虚膏脂转输障碍的启动因素,以其介导“炎症-代谢”紊乱为切入点,深入揭示血脂异常疾病发病机制以提高中西医结合防治水平逐渐成为基础和临床研究焦点和热点。从肠源性高密度脂蛋白(high-density lipoprotein 3,HDL3)调控脂多糖(lipopolysaccharide,LPS)介导Kupffer-肝细胞Cross-talk的角度,深入分析脾虚膏脂转输障碍的分子生物学机制,揭示健脾化浊法的效应机制,为临床防治血脂异常提供科学依据。

关键词：肠源性HDL3 脂多糖 Kupffer细胞肝细胞 Cross-talk

Cross-talk between T-cells and gut-microbiota in neurodegenerative disorders

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2019年第12期14卷 2091-2092页

作者： Rodrigo Pacheco Departamento de Ciencias Biológicas Facultad de Ciencias de la Vida Universidad Andres Bello Laboratorio de Neuroinmunología Fundación Ciencia & Vida

The emerging role of gut microbiota as a key player in the development of neurodegenerative disorders: Mammals have evolved together with commensal microbiota to establish a symbiotic relationship in which they regulate reciprocally by synthesizing and responding to several common chemical substances. In this regard, gut microbiota constitutes a consortium of bacteria that not only participates in the degradation of nutrients, but also produces metabolites, fatty acids and neurotransmitters that can act on the enzymes and receptors expressed in eukaryotic cells, which considerably affects the physiology of the host and contribute to maintaining homeostasis (Lyte, 2013).

关键词： Cross-talk T-cells neurodegenerative disorders

Beam test of signal Cross-talk and transmission for LMPRC

在线全文

学校读者我要写书评

暂无评论

Chinese Physics C 2011年第9期35卷 838-843页

作者：孙勇杰李澄唐泽波徐来林陈天翔邵明 Department of Modern Physics University of Science and Technology of China(USTC)

A new prototype of large area Multi-gap Resistive Plate Chamber （MRPC） with long readout strips was built. This Long-strip Multi-gap Resistive Plate Chamber （LMRPC） is double stacked and has ten 250 μm-thick gas gaps. Signals are read out from the two ends of each strip with an active area of 50 cmx 2.5 cm in each. The detector was tested at FOPI in GSI, using the secondary particles of proton beams （E = 3.5 GeV） colliding with a Pb target. The results show that the LMRPC prototype has a time resolution of about 60- 70 ps; the detecting efficiency is over 98% and the ratio of Cross-talk is lower than 2%. The detector also has a good spatial resolution of 0.36 cm along the strip direction.

关键词： LMRPC readout method Cross-talk

Cross-talk between MEK1/2-ERK1/2 signaling and G protein-couple signaling in synoviocytes of collagen-induced arthritis rats

在线全文

学校读者我要写书评

暂无评论

Chinese Medical Journal 2008年第22期121卷 2278-2283页

作者： ZHANG Ling-ling WEI Wei WANG Qing-tong CHEN Jing-yu CHEN Yin Institute of Clinical Pharmacology Anhui Medical University Key Laboratory of Anti-inflammatory and Immuno-pharmacology in Anhui Province Key Laboratory of Research & Development of Chinese Medicine in Anhui Province Hefei Anhui 230032 China

Background Signaling pathways that regulate the production of cytokines and destructive enzymes have been implicated in rheumatoid arthritis （RA） pathogenesis. There are co-relations between signaling pathways. The aim of this study was to investigate interactions and Cross-talks between MEK1/2-extracellular signal-related kinase （ERK1/2） signaling and G protein-couple signaling in synoviocytes of collagen-induced arthritis （CIA） rats by the stimulation of interleukin-1 （IL-1）, U0126, isoprenaline hydrochloride and aminophyline respectively. Methods Twenty Sprague-Dawley （SD） rats were induced by chicken type II collagen. Synoviocytes of CIA rats were isolated and cultured. The expressions of Gi, phosphorylated MEK1/2 （p-MEK1/2） and phosphorylated ERK1/2 （p-ERK1/2） were detected by Western blotting, cAMP level and protein kinase A （PKA） activity were measured by radioimmunoassay and kinase-glo luminescent kinase assay respectively. Results There was remarkable inflammation in CIA rats accompanied by swelling paws, hyperplastic synovium, pannus and cartilage erosion, cAMP level and PKA activity of synoviocytes decreased. Gi, p-ERK1/2 and p-MEK1/2 increased, rlL-la improved the expression of Gi, p-ERK1/2 and p-MEK1/2, cAMP and PKA increased with stimulation of rlL-1α. U0126 inhibited Gi, cAMP and PKA of synoviocytes stimulated by rlL-la. Isoprenaline hydrochloride enhanced Gi, cAMP and PKA, but had no effects on p-MEK1/2 and p-ERK1/2. Aminophyline increased cAMP and PKA, but inhibited p-MEK1/2 and p-ERK1/2. Conclusions Mitogen-activated protein kinases （MAPKs） and G protein-couple signaling are associated with synovitis. There are cross talks between MAPKs and G protein-couple signaling. The two signaling pathways represent potential therapeutic targets for RA.

关键词： arthritis signaling Cross-talk G protein MAP kinases

Cross-talk between Extracellular S1P/S1P2 and P42/44 MAPK in bcr/abl Positive Chronic Myeloid Leukemia Cells

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Cancer Research 2009年第1期21卷 20-27页

作者： Yin Xu Fei-qun Zheng Qun-wei Zhang Yang Liu Hai-feng Duan Chun-ping Cui Bin Wu Yi-de Qin Deparment of Biochemistry and Molecular Biology Anhui Medical University Hefei 230032 China Department of Experimental Hematology Beijing Institute of Radiation Medicine Beijing 100850 China Key Laboratory of Carcinogenesis and Translational Research （Ministry of Education） Department of lnterventional Therapy Peking University School of Oncology Beijing Cancer Hospital ＆ Institute Beijing 100142 China

Objective： BCR/ABL oncoprotein-expression is associated with uncontrolled cell growth. Sphingosine kinase 1 （SPK1） regulates the production of sphingosine 1-phosphate （S1P）, a key lipid signal molecular in cell proliferation and survival. The objective of this study was to elucidate the roles of S1P and its receptors in bcr/abl positive chronic myeloid leukemia （CML） cells. Methods： The expressions of SIP receptors： S1P1, S1P2 and S1P3 in CML cells were detected by RT-PCR. SPK1 expression, activity and extracellular S1P were determined in ECV304 and HL-60 cells which were transfected with bcr/abl gene. To elucidate the relationship between the BCR/ABL, ERK/MAPK （extracellular signal-regulated kinase/mitogen-activated protein kinase）, SPK/S 1P and S 1P/S 1 P2 signal pathways, bcr/abl positive CML cell line K562 was treated with STI571, PD98059, N,N-dimethyl sphingosine （DMS） and JTE-013. Results： Retrovirus-mediated overexpression of bcr/abl gene in ECV304 and HL-60 cells resulted in upregulation of the expression, activity of SPK1 and increase of the secretion of SIP, whereas treatment of STI571 and PD98059 decreased the BCR/ABL-induced S1P secretion. Treatment of DMS reduced S1P secretion and P42/44MAPK phosphorylation. S1P2-selective antagonist JTE-013 could also decrease P42/44MAPK phosphorylation. Conclusion： These results suggest that BCR/ABL up-regulates extracellular sphingosine 1-phosphate through sphingosine kinase 1 and there is Cross-talk between SPK1/S1P/S1P2 and P42/44MAPK in bcr/abl positive CML cells.

关键词： Sphingosine kinase Sphingosine 1-phosphate CML Extracellular signal-regulated kinase/mitogen-activated protein kinase Cross-talk

基于GEO芯片联合网络药理学探讨当归饮子治疗慢性荨麻疹的Cross-talk及miRNA-mRNA机制

在线全文

学校读者我要写书评

暂无评论

云南中医学院学报 2020年第4期43卷 79-86页

作者：包成通魏歆然张依依钟峰匡泓俊牛子青刘小娟王璐李俊熙章薇湖南中医药大学湖南长沙410208 贵州中医药大学贵州贵阳550025 湖南中医药大学第一附属医院湖南长沙410007

目的采用GEO芯片联合网络药理学的研究方法,初步探讨当归饮子治疗慢性荨麻疹(CU)的Cross-talk及miRNA-mRNA机制,为后续实验研究提供理论基础。方法采用R语言limma包对GSE57178芯片进行差异分析;采用TCMSP、TCMID、PubChem、String、DAVI... 详细信息

目的采用GEO芯片联合网络药理学的研究方法,初步探讨当归饮子治疗慢性荨麻疹(CU)的Cross-talk及miRNA-mRNA机制,为后续实验研究提供理论基础。方法采用R语言limma包对GSE57178芯片进行差异分析;采用TCMSP、TCMID、PubChem、String、DAVID、TargetScan数据库对当归饮子有效成分、潜在靶点、PPI、KEGG、Cross-talk及miRNA-mRNA机制进行预测分析。结果GSE57178差异分析得出257个差异基因,其中240个基因表达上调(如:HIF1A、MMP12、STAT3等);有17个基因表达下调(如:FABP7、KRT15、LGR5等),其中有36个当归饮子治疗慢性荨麻疹的潜在靶点,拓扑分析出24个关键靶点基因在其中发挥重要作用;Cross-talk分析得出1个靶点(SELE)在TNF通路与细胞粘附分子(CAMs)2条通路中“串话”;1个靶点(ITGB2)在吞噬体与CAMs 2条通路中“串话”;2个靶点(CTSL、CTSS)在抗原处理表达、吞噬体与溶酶体3条通路中“串话”;2个靶点(VCAM1、ICAM1)在NF-kappa B、TNF与CAMs 3条通路中“串话”;1个靶点(TLR4)在HIF-1、NF-kappa B、吞噬体3条通路中“串话”;miRNA-mRNA预测分析得出24个潜在靶点mRNA,构成了477组miRNA-mRNA模式,在CU炎症反应中发挥作用。结论本研究从基因、分子角度预测和分析了当归饮子治疗CU的多靶点、多Cross-talk、多miRNA-mRNA作用机制,为后续研究提供了依据,可成为未来机制研究的新方向。

关键词： GEO 当归饮子慢性荨麻疹网络药理学 Cross-talk miRNA

内质网应激下肝癌细胞Akt和ERK通路间的Cross-talk研究

在线全文

学校读者我要写书评

暂无评论

中国现代医学杂志 2011年第20期21卷 2361-2364页

作者：严冬梅李敏军刘友平段春燕泸州医学院生物化学教研室四川泸州646000 泸州医学院数学与信息技术教研室四川泸州646000

目的研究内质网应激介导的肝癌细胞PI3K/AKt和M EK/ER K途径间的信号交流。方法采用PI3K抑制剂LY 294002/Akt激活型突变载体m yr-Akt和M EK抑制剂U 0126分别阻断/激活内质网应激介导的Akt和ER K活化,并利用W estern blot分析内质网应激... 详细信息

目的研究内质网应激介导的肝癌细胞PI3K/AKt和M EK/ER K途径间的信号交流。方法采用PI3K抑制剂LY 294002/Akt激活型突变载体m yr-Akt和M EK抑制剂U 0126分别阻断/激活内质网应激介导的Akt和ER K活化,并利用W estern blot分析内质网应激条件下PI3K/Akt和M EK/ER K途径间的信号交流。结果阻断PI3K/Akt明显促进内质网应激介导的M EK/ER K活化,而过度激活PI3K/Akt则抑制内质网应激介导的M EK/ER K活化。阻断M EK/ER K对内质网应激介导的PI3K/Akt活化无影响。结论 PI3K/Akt和M EK/ER K信号途径间在内质网应激肝癌细胞中存在信号交流。

关键词：内质网应激 Akt ERK Cross-talk 肝癌细胞

Testing Cross-talk Induced Delay Faults in Digital Circuit Based on Transient Current Analysis

在线全文

学校读者我要写书评

暂无评论

Wuhan University Journal of Natural Sciences 2006年第6期11卷 1445-1448页

作者： WANG Youren DENG Xiaoqian CUI Jiang YAO Rui ZHANG Zhai College of Automation Engineering Nanjing University of Aeronautics and Astronautics Nanjing 210016 Jiangsu China

The delay fault induced by Cross-talk effect is one of the difficult problems in the fault diagnosis of digital circuit. An intelligent fault diagnosis based on IDDT testing and support vector machines （SVM） classifier was proposed in this paper. Firstly, the fault model induced by Cross-talk effect and the IDDT testing method were analyzed, and then a delay fault localization method based on SVM was presented. The fault features of the sampled signals were extracted by wavelet packet decomposition and served as input parameters of SVM classifier to classify the different fault types. The simulation results illustrate that the method presented is accurate and effective, reaches a high diagnosis rate above 95%.

关键词： delay fault Cross-talk fault localization digital circuit IDDT SVM