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Development of a stable attenuated double-mutant of tobacco mosaic virus for cross-protection

Journal of Integrative Agriculture 2024年第7期23卷 2318-2331页

作者： Xiaojie Xu Shaoyan Jiang Chunju Liu Xujie Sun Qing Zhu Xiuzhai Chen Pengchao Jiang Fenglong Wang Yanping Tian Xiangdong Li The Engineering Research Institute of Agriculture and Forestry Ludong UniversityYantai 264025China Laboratory of Plant Virology Department of Plant PathologyCollege of Plant ProtectionShandong Agricultural UniversityTai’an 271018China Weifang Tobacco Corporation Weifang 261031China Linyi Tobacco Corporation Linyi 276000China Tobacco Research Institute Chinese Academy of Agricultural SciencesQingdao 266101China

Tobacco(Nicotiana tabacum)and tomato(Solanum lycopersicum)are two major economic crops in *** mosaic virus(TMV;genus Tobamovirus)is the most prevalent virus infecting both ***,some widely cultivated tobacco and tomato cultivars are susceptible to TMV and there is no effective strategy to control this ***-protection can be a safe and environmentally friendly strategy to prevent viral ***,stable attenuated TMV mutants are *** this study,we found that the substitutions in the replicase p126,arginine at position 196(R^(196))with aspartic acid(D),glutamic acid at position 614(E^(614))with glycine(G),serine at position 643(S^(643))with phenylalanine(F),or D at position 730(D^(730))with S,significantly reduced the virulence and replication of ***,only the mutation of S^(643) to F reduced the RNA silencing suppression activity of TMV p126.A double-mutant TMV-E614G-S643F induced no visible symptom and was genetically stable through six successive passages in tobacco ***,our results showed that TMV-E614G-S643F double-mutant could provide effective protection against the wild-type TMV infection in tobacco and tomato *** study reports a promising mild mutant for cross-protection to control TMV in tobacco and tomato plants.

关键词： cross-protection double-mutant p126 tobacco mosaic virus virulence

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Improving cross-protection against Influenza Virus Using Recombinant Vaccinia Vaccine Expressing NP and M2 Ectodomain Tandem Repeats

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Virologica Sinica 2019年第5期34卷 583-591页

作者： Wenling Wang Baoying Huang Xiuping Wang Wenjie Tan Li Ruan NHC Key Laboratory of Biosafety National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention

Conventional influenza vaccines need to be designed and manufactured ***,they occasionally provide poor protection owing to antigenic ***,there is an urgent need to develop universal vaccines against influenza *** nucleoprotein(NP)and extracellular domain of matrix protein 2(M2e)genes from the influenza A virus A/Beijing/30/95(H3N2),we constructed four recombinant vaccinia virus-based influenza vaccines carrying NP fused with one or four copies of M2e genes in different *** recombinant vaccinia viruses were used to immunize BALB/C *** and cellular responses were measured,and then the immunized mice were challenged with the influenza A virus A/Puerto Rico/8/34(PR8).NP-specific humoral response was elicited in mice immunized with recombinant vaccinia viruses carrying full-length NP,while robust M2e-specific humoral response was elicited only in the mice immunized with recombinant vaccinia viruses carrying multiple copies of *** recombinant viruses elicited NP-and M2e-specific cellular immune responses in *** immunization with RVJ-4M2eNP induced remarkably higher levels of IL-2 and IL-10 cytokines specific to ***,RVJ-4M2eNP immunization provided the highest cross-protection in mice challenged with 20 MLD5〇of ***,the cross-protection potentially correlates with both NP and M2e-specific humoral and cellular immune responses induced by RVJ-4M2eNP,which expresses a fusion antigen of full-length NP preceded by four M2e *** results suggest that the rational fusion of NP and multiple M2e antigens is critical toward inducing protective immune responses,and the 4M2eNP fusion antigen may be employed to develop a universal influenza vaccine.

关键词： Influenza A virus(IAV) cross-protection Recombinant vaccinia virus Conserved antigen

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Modeling the effects of cross-protection control in plant disease with seasonality

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International Journal of Biomathematics 2017年第6期10卷 277-300页

作者： Shujing Gao Lijun Xia Jialin Wang Zujin Zhang Key Laboratory of Jiangxi Province for Numerical Simulation and Emulation Techniques Gannan Normal University Ganzhou 341000 P. R. China Department of Basic Education Ji'an Polytechnic Ji'an 343000 P. R. China Key Laboratory of Jiangxi Province for Numerical Simulation and Emulation Techniques Cannan Normal University Ganzhou 341000 P. R. China

cross-protection in plants has been widely used to control losses caused by virus diseases in the world. Here, a non-autonomous plant-virus disease model was developed includ- ing cross-protection. Global dynamics of the model was discussed. Under the quite weak assumptions, integral form conditions were resolved for permanence of the system and extinction of diseases. Furthermore, we looked into the sufficient conditions that plants could be protected against the detrimental effects of infection by an infection with the mild virus isolates. Last, we performed numerical simulations. Our investigations sug- gested that cross-protection played an important role in controlling the spread of the challenging virus in plants.

关键词： Plant virus cross-protection seasonality permanence extinction.

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In search of a pan-coronavirus vaccine:next-generation vaccine design and immune mechanisms

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Cellular & Molecular Immunology 2024年第2期21卷 103-118页

作者： S.Cankat M.U.Demael L.Swadling Division of Infection and Immunity Institute of Immunity and TransplantationUniversity College LondonPears BuildingLondon NW32PPUK

Members of the coronaviridae family are endemic to human populations and have caused several epidemics and pandemics in recent *** this review,we will discuss the feasibility of and progress toward the ultimate goal of creating a pan-coronavirus vaccine that can protect against infection and disease by all members of the coronavirus *** will detail the unmet clinical need associated with the continued transmission of SARS-CoV-2,MERS-CoV and the four seasonal coronaviruses(HCoV-OC43,NL63,HKU1 and 229E)in humans and the potential for future zoonotic *** will highlight how first-generation SARS-CoV-2 vaccines and natural history studies have greatly increased our understanding of effective antiviral immunity to coronaviruses and have informed next-generation vaccine *** will then consider the ideal properties of a pan-coronavirus vaccine and propose a blueprint for the type of immunity that may offer ***,we will describe a subset of the diverse technologies and novel approaches being pursued with the goal of developing broadly or universally protective vaccines for coronaviruses.

关键词： Vaccine Coronavirus cross-reactive immunity cross-protection Universal

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An asymptomatic geminivirus activates autophagy and enhances plant defenses against diverse pathogens

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Stress Biology 2024年第1期4卷 156-173页

作者： Li Wang Zijie Yu Mengge Jiang Mengyuan Tian Hongsheng Zhou Wanying Zhao Ida Bagus Andika Qiaoxia Shang Liying Sun State Key Laboratory of Crop Stress Resistance and High-Efficiency Production College of Plant ProtectionNorthwest A&F UniversityYangling 712100China College of Plant Health and Medicine Qingdao Agricultural UniversityQingdao 266109China College of Bioscience and Resource Environment Key Laboratory for Northern Urban Agriculture of Ministry of Agriculture and Rural AffairsBeijing University of AgricultureBeijing 102206China

Plant viral diseases cause great losses in agricultural *** cross-protection is a strategy in which a mild virus is employed to shield plants against subsequent infections by severe viral ***,this approach is restricted to protection against the same *** this study,we observed that pre-inoculation with apple geminivirus(AGV)reduced the accumulation of secondarily infected heterologous viruses,such as cucumber mosaic virus,potato virus X,and tobacco mosaic virus in Nicotiana benthamiana,tomato,and pepper *** expression analysis showed that autophagy-related genes were transcriptionally up-regulated upon AGV inoculation at an early stage of ***,autophagic activity was observed to be elevated following AGV ***,AGV accumulation was reduced in autophagy-deficient plants,suggesting that autophagy activation promotes AGV infection in the ***,pre-inoculation with AGV provided cross-protection against infection with a phytopathogenic bacterium(Pseudomonas syringae)and fungus(Botrytis cinerea)in Nicotiana *** summary,our study showed that AGV,an asymptomatic virus,could protect plants against severe viral,fungal,and bacterial diseases to some extent through the activation of autophagy pathways,highlighting its potential as a biocontrol agent for managing a wide range of plant crop diseases in the field.

关键词： Apple geminivirus Autophagy cross-protection Pathogens Resistance

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Inhibition of Japanese Encephalitis Virus Infection by Flavivirus Recombinant E Protein Domain Ⅲ

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Virologica Sinica 2013年第3期28卷 152-160页

作者： Jingjing Fan Yi Liu Xuping Xie Bo Zhang Zhiming Yuan Key Laboratory of Agricultural and Environmental Microbiology Wuhan Institute of Virology Chinese Academy of Sciences

Japanese encephalitis virus (JEV) is a mosquito-borne flavivirus closely related to the human pathogens including yellow fever virus, dengue virus and West Nile virus. There are currently no effective antiviral therapies for all of the flavivirus and only a few highly effective vaccines are licensed for human use. In this paper, the E protein domain III (DIII) of six heterologous flaviviruses (DENV1-4, WNV and JEV) was expressed in Escherichia coli successfully. The proteins were purified after a solubilization and refolding procedure, characterized by SDS-PAGE and Western blotting. Competitive inhibition showed that all recombinant flavivirus DIII proteins blocked the entry of JEV into BHK-21 cells. Further studies indicated that antibodies induced by the soluble recombinant flavivirus DIII partially protected mice against lethal JEV challenge. These results demonstrated that recombinant flavivirus DIII proteins could inhibit JEV infection competitively, and immunization with proper folding flavivirus DIII induced cross-protection against JEV infection in mice, implying a possible role of DIII for the cross-protection among flavivirus as well as its use in antigens for immunization in animal models.

关键词： Japanese encephalitis virus E protein domain III cross-protection Antibody

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An Attenuated Strain of Cucumber Green Mottle Mosaic Virus as a Biological Control Agent against Pathogenic Viral Strains

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American Journal of Plant Sciences 2016年第5期7卷 724-732页

作者： Anna A. Slavokhotova Ekaterina A. Istomina Emma N. Andreeva Tatyana V. Korostyleva Vitalij A. Pukhalskij Alexander N. Shijan Tatyana I. Odintsova Vavilov Institute of General Genetics Russian Academy of Sciences Moscow Russia

Cucumber green mottle mosaic virus (CGMMV), a member of the Tobamovirus genus, causes a severe disease of cucurbits. In the Moscow region of Russian Federation, the incidence of infection on cucumber plants in greenhouses is high;however, the virus is poorly studied. In this work, the full-length genomes of two pathogenic MC-1 and MC-2 strains of CGMMV isolated from cucumber plants grown in greenhouses in the Moscow region and the attenuated VIROG-43M strain were sequenced. Comparison of VIROG-43M nucleotide sequence with those of the pathogenic strains revealed three missense mutations. Their role in attenuation is discussed. For the first time, in a number of trials conducted under laboratory conditions and in commercial greenhouses, the efficiency of the attenuated VIROG-43M strain as a biocontrol agent for cucumber plant protection resulting in significant yield gain was demonstrated. Phylogenetic analysis with 83 full-length CGMMV coat protein genes isolated in 16 different countries showed that Russian strains are related to isolates from Spain, Greece, USA and Israel.

关键词： Cucumber Green Mottle Mosaic Virus Tobamovirus Attenuated Virus Strain cross-protection Biological Control

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The optimal immunization procedure of DNA vaccine pcDNA-TA4-IL-2 of Eimeria tenella and its cross-immunity to Eimeria necatrix and Eimeria acervulina

The optimal immunization procedure of DNA vaccine pcDNA-TA4-...

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中国畜牧兽医学会家畜寄生虫学分会第六次代表大会暨第十次学术研讨会

作者： Song-Xiaokai,Xu-Lixin,Yan-Ruofeng,Xu-Qianming,Huang-Xinmei,Li-Xiangrui~* (College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,PR China)

Fusion DNAvaccines co-expressed Eimeria tenella TA4 and chicken IL-2(chIL-2) were constructed and their efficacy against *** challenge were *** immunization procedure of DNA vaccine pcDNA-TA4-IL-2 of Eimeria tenella,including route,dose,time of immunization and age of primary immunization of chicken,was optimized and the stability and the cross-species protection of the vaccine were also *** of immunization was evaluated on the basis of oocyst decrease ratio,lesion score,body-weight gain and the anti-coccidial index(ACI).Results indicated DNA vaccines pcDNA3.1-TA4-IL-2,pcDNA3.1- TA4 and pcDNA4.0c-IL-2 were successfully constructed and the antigen genes could be expressed effectively in *** animal experimental results showed that DNA vaccines could obviously alleviate cecal lesions, body weight loss and increase oocyst decrease *** ACI of pcDNA3.0-TA4-IL-2 group was 192,higher than that of pcDNA3.1-TA4 *** optimization of immunization procedure of DNA vaccine pcDNA-TA4- IL-2 showed that 25μg was the optimal dose and intramuscular injection was the most effective route to induce protective *** were no significant differences of ACIs between boosting and non-boosting *** time and temperature had little effect on the immunizing efficacy of the *** vaccine could provide partial cross-protection against the challenge with *** and ***,but not with E. maxima.

关键词： E.tenella TA4 DNA vaccine chIL-2 immunization procedure cross-protection

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Strengthening global health resilience:Marburg virus-like particle vaccines and the One Health approach

Science in One Health

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Science in One Health 2024年第1期3卷 35-41页

作者： Ram Bahadur Khadka Khimdhoj Karki Jitendra Pandey Rabin Gyawali Gautam Prasad Chaudhary Department of Laboratory Science Crimson College of TechnologyAffiliated with Pokhara UniversityButwal-11DevinagarRupandehi 32907Nepal Department of Chemistry University of Hawaii at ManoaHonoluluHIUSA Padmodaya Campus Affiliated to Tribhuwan UniversityDang 21906Nepal Department of Pharmacy Crimson College of TechnologyAffiliated with Pokhara UniversityButwal-11DevinagarRupandehi 32907Nepal

The Marburg virus(MARV),belonging to the Filoviridae family,poses a significant global health threat,emphasizing the urgency to develop Marburg virus-like particle(VLP)vaccines for outbreak *** virus's menacing traits accentuate the need for such vaccines,which can be addressed by VLPs that mimic its structure safely,potentially overcoming past *** Marburg vaccine endeavors and their challenges are examined in the historical perspectives section,followed by an exploration of VLPs as transformative tools,capable of eliciting immune responses without conventional *** milestones and achievements in Marburg VLP vaccine development,seen through preclinical and clinical trials,indicate potential *** challenges,encompassing durability,strain diversity,and equitable distribution,are addressed,with proposed innovations like novel adjuvant,mRNA technology,and structure-based design poised to enhance Marburg VLP *** review highlights the transformative potential of Marburg VLPs in countering the virus,showcasing global collaboration,regulatory roles,and health equity for a safer future through the harmonious interplay of science,regulation,and global efforts.

关键词： Marburg virus Virus-like particle vaccine cross-protection mRNA technology Global collaboration

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