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An Attenuated Highly Pathogenic Chinese PRRS Viral Vaccine Confers cross protection to Pigs against Challenge with the Emerging PRRSV NADC30-Like Strain

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Virologica Sinica 2018年第2期33卷 153-161页

作者： Hewei Zhang Mingqi Xia Wei Wang Decai Ju Long Cao Bai Wu Xin Wang Ying Wu Ni Song Jiaxin Hu Changxiao Tian Shucheng Zhang Hua Wu Sinovet (Beijing) Biotechnology Co. Ltd Beijing 100085 China State Key Laboratory for Molecular Biology of Special Economic Animals Institute of Special Economic Animal and Plant Sciences Chinese Academy of Agricultural Sciences Changchun 130112 China

A novel PRRSV strain was isolated in China that was genetically similar to the NADC30 strain which is reported to have spread throughout China. The objective of the present study was to evaluate the cross-protective efficacy of the live vaccine TJM-F92 in young pigs against challenge with a NADC30-like strain, HN201605. Twenty-five PRRSV-and antibody-free pigs were randomly divided into the following five groups: Vac/ChA, Unvac/ChA, Vac/ChB, Unvac/ChB and the *** pigs in groups Vac/ChA and Vac/ChB were inoculated intramuscularly with 1 mL TJM-F92(10^(5.0) TCID_(50)/mL). At28 days post vaccination(0 days post challenge), groups Vac/ChA and Unvac/ChA were inoculated intranasally with 10^(4.5) TCID_(50)/mL PRRSV strain TJ F3(2 mL/pig), while groups Vac/ChB and Unvac/Ch B were inoculated, using the same route, with the same dose of the NADC30-like strain HN201605 F3. Protective effects of the PRRSV strain were observed in all pigs in the Vac/ChA and Vac/ChB groups. Neither high fever nor signs of clinical disease were observed through the experiment in these groups, whereas pigs in Unvac/ChA group exhibited serious clinical symptoms, pathological lesions,and weight loss. In Unvac/ChB group, pigs developed milder clinical symptoms, which demonstrated that the NADC30-like strain HN201605 had moderate pathogenicity. The results suggest that the MLV vaccine strain TJM-F92 is an effective and safe vaccine candidate for use in China.

关键词： Porcine reproductive and respiratory syndrome virus (PRRSV) NADC30-like Vaccine cross protection

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Development and evaluation of neutralizing antibodies for cross-protection against West Nile virus and Japanese encephalitis virus

Infectious Medicine

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Infectious Medicine 2023年第3期2卷 212-223页

作者： Meng-Jie Yang Hao-Ran Luo Zhen-Yu Fan Yu-Xiang Feng Ning Wei Bi-Bo Zhu Jing Ye Sheng-Bo Cao You-Hui Si National Key Laboratory of Agricultural Microbiology College of Veterinary MedicineHuazhong Agricultural UniversityWuhan 430070China Hubei Hongshan Laboratory Wuhan 430070China Frontiers Science Center for Animal Breeding and Sustainable Production Huazhong Agricultural UniversityWuhan 430070China The Cooperative Innovation Center for Sustainable Pig Production Huazhong Agricultural UniversityWuhan 430070China

Background:West Nile virus is a severe zoonotic pathogen that can cause severe central nervous system symptoms in humans and horses,and is fatal for birds,chickens and other *** no specific drugs or vaccines available,antibody-based therapy is a promising *** study aims to develop neutralizing antibodies against West Nile virus and assess their cross-protective potential against Japanese encephalitis ***:Monoclonal antibodies against WNV and JEV were isolated by hybridoma *** therapeutic efficacy of these antibodies was evaluated using a mouse model,and a humanized version of the monoclonal antibody was generated for potential human ***:In this study,we generated eight monoclonal antibodies that exhibit neutralizing activity against *** therapeutic effects against WNV were validated both in vivo and in *** these antibodies,C9-G11-F3 also exhibited cross-protective activity against *** also humanized the antibody to ensure that it could be used for WNV infection treatment in ***:This study highlights the importance of neutralizing antibodies as a promising approach for pro-tection against West Nile virus infection and suggests their potential utility in the development of therapeutic interventions.

关键词： West Nile virus Neutralizing antibodies cross protection Therapeutic antibodies Humanization

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Virologica Sinica 2023年第5期38卷 813-826页

作者： Yang Li Lele Xu Dian Jiao Zifang Zheng Zhihao Chen Yang Jing Zhiwei Li Zhiqian Maa Yingtong Feng Xuyang Guo Yumiao Wang Yuan He Haixue Zheng Shuqi Xiao State Key Laboratory for Animal Disease Control and Prevention College of Veterinary MedicineLanzhou UniversityLanzhou Veterinary Research InstituteChinese Academy of Agricultural SciencesLanzhou730046China College of Veterinary Medicine Northwest A&F UniversityYangling712100China

Porcine reproductive and respiratory syndrome(PRRS)is one of the most significant diseases affecting the pig industry *** PRRSV mutation rate is the highest among the RNA *** date,NADC30-like PRRSV and highly pathogenic PRRSV(HP-PRRSV)are the dominant epidemic strains in China;however,commercial vaccines do not always provide sufficient cross-protection,and the reasons for insufficient protection are *** study isolated a wild-type NADC30-like PRRSV,SX-YL1806,from Shaanxi *** challenge experiments in piglets showed that commercial modified live virus(MLV)vaccines provided good protection against ***,it could not provide sufficient protection against the novel strain *** explore the reasons for this phenomenon,we compared the genomic homology between the MLV strain and HP-PRRSV or NADC30-like PRRSV and found that the MLV strain had a lower genome similarity with NADC30-like *** neutralization assay showed that MLV-immune serum slightly promoted the homologous HP-PRRSV replication and significantly promoted the heterologous NADC30-like PRRSV strain replication in vitro,suggesting that antibody-dependent enhancement(ADE)might also play a role in decreasing MLV protective *** findings expand our understanding of the potential factors affecting the protective effect of PRRSV MLV vaccines against the NADC30-like strains.

关键词： NADC30-like PRRSV Modified live virus(MLV)vaccines Genomic similarity Antibody dependent enhancement(ADE) cross protection

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Preparation and development of equine hyperimmune globulin F（ab'）2 against severe acute respiratory syndrome coronavirus

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Acta Pharmacologica Sinica 2005年第12期26卷 1479-1484页

作者： Jia-hai LU~(2,8,9) Zhong-min GUO~(2,9) Wen-yu HAN~(3,9) Guo-ling WANG~(2,9) Ding-mei ZHANG~2 Yi-fei WANG~4 Sheng-yun SUN~4 Qin-he YANG~4 Huan-ying ZHENG~5 Bing L WONG~6 Nan-shan ZHONG~7 2 Sun Yat-sen University,Guangzhou 510080,China 3 The Agriculture School of Jilin University,Changchun 130062,China 4 Ji-nan University,Guangzhou 510632,China 5 Center for Disease Control and Prevention of Guangdong Province,Guangzhou 510300,China 5 Advantek Biologics,Hong Kong,China 7 Guangzhou Institute of Respiratory Diseases,Guangzhou Medical College,Guangzhou 510120,China Sun Yat-sen University Guangzhou 510080 China. jiahailu@***

Aim:The resurgence of severe acute respiratory syndrome(SARS)is still a threatbecause the causative agent remaining in animal reservoirs is not fully understood,and sporadic cases continue to be *** high titers of anti-SARShyperimmune globulin to provide an alternative pathway for emergent future pre-vention and treatment of ***:SARS coronavims(CoV)F69(AY313906)and Z2-Y3(AY394989)were isolated and identified from 2 different Cantoneseonset SARS *** was prepared from SARS-CoV F69 *** horses were immunized 4 times and serum was collected periodically tomeasure the profile of specific IgG and neutralizing antibodies using indirect en-zyme-linked immunosorbent assay and a microneutralization *** were col-lected in large amounts at the peak,where IgG was precipitated using ammoniumsulphate and subsequently digested with *** product was then purifiedusing anion-exchange chromatography to obtain F(ab′)2 fragments,Results:Thespecific IgG and neutralizing antibody titers peaked at approximately week 7 afterthe first immunization,with a maximum value of 1:*** sera collected at thepeak were then *** of approximately 15 g F(ab′)2 was obtained from1 litre antiserum and the purity was above 90% with the titer of 1:5120,which couldneutralize the other strain(SARS-CoV Z2-Y3)as ***:This researchprovides a viable strategy for the prevention and treatment of SARS coronavirusinfection with equine hyperimmune globulin,with the purpose of combating anyresurgence of SARS.

关键词： severe acute respiratory syndrome coronavirus neutralizing antibodies hyperimmune globulin cross protection F(ab’)2 fragments

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Seasonal influenza vaccination may mitigate the potential impact of an H5N1 pandemic

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Chinese Medical Journal 2008年第15期121卷 1481-1483页

作者： QIN Cheng-feng QIN E-de State Key Laboratory of Pathogen and Biosecurity Institute of Microbiology and Epidemiology Academy of Military Medical Sciences Beijing 100071 China

Seasonal influenza is a highly contagious, acute respiratory illness that affects people of all ages. The major pathogens, influenza A viruses, are classified into serologically defined antigenic subtypes of the hemagglutinin （HA） and neuraminidase （NA）. Of 16 identified HA and 9 NA subtypes, only H1N1 and H3N2 subtypes are now circulating among humans. Influenza vaccines have been available for over 60 years and well proven to be an effective public health intervention to control seasonal influenza epidemics. Seasonal influenza vaccines presently available, inactivated or split, contain the circulating strains of influenza A virus H3N2, H1N1 and influenza B virus. The composition of the vaccine is renewed semi-annually, as necessary, based on surveillance data.

关键词： influenza A virus pandemic vaccine neuraminidase cross protection

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