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Strain and process engineering toward continuous industrial fermentation

Frontiers of Chemical Science and Engineering 2023年第10期17卷 1336-1353页

作者： Yufei Dong Ye Zhang Dehua Liu Zhen Chen Key Laboratory of Industrial Biocatalysis(Ministry of Education) Department of Chemical EngineeringTsinghua UniversityBeijing 100084China Tsinghua Innovation Center in Dongguan Dongguan 523808China Center for Synthetic and Systems Biology Tsinghua UniversityBeijing 100084China

Most current biotechnology industries are based on batch or fed-batch fermentation processes,which often show low productivity and high production costs compared to chemical processes.To increase the economic competitiveness of biological processes,continuous fermentation technologies are being developed that offer significant advantages in comparison with batch/fed-batch fermentation processes,including:(1)removal of potential substrates and product inhibition,(2)prolonging the microbial exponential growth phase and enhancing productivity,and(3)avoiding repeated fermentation preparation and lowering operation and installation costs.However,several key challenges should be addressed for the industrial application of continuous fermentation processes,including(1)contamination of the fermentation system,(2)degeneration of strains,and(3)relatively low product titer.In this study,we reviewed and discussed metabolic engineering and synthetic biology strategies to address these issues.

关键词： continuous fermentation productivity contamination strain degeneration metabolic engineering

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Mass Transfer, Gas Holdup, and Kinetic Models of Batch and continuous fermentation in a Novel Rectangular Dynamic Membrane Airlift Bioreactor

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Engineering 2022年第6期8卷 153-163页

作者： Ganlu Li Kequan Chen Yanpeng Wei Jinlei Zeng Yue Yang Feng He Hui Li Pingkai Ouyang College of Biotechnology and Pharmaceutical Engineering Nanjing Tech UniversityNanjing 211816China Jiangsu Jicui Industrial Biotechnology Research Institute Co. Ltd.Nanjing 211800China

Compared with conventional cylinder airlift bioreactors(CCABs)that produce coarse bubbles,a novel rectangular dynamic membrane airlift bioreactor(RDMAB)developed in our lab produces fine bubbles to enhance the volumetric oxygen mass transfer coefficient(k_(L)a)and gas holdup,as well as improve the bioprocess in a bioreactor.In this study,we compared mass transfer,gas holdup,and batch and con-tinuous fermentation for RNA production in CCAB and RDMAB.In addition,unstructured kinetic models for microbial growth,substrate utilization,and RNA formation were established.In batch fermentation,biomass,RNA yield,and substrate utilization in the RDMAB were higher than those in the CCAB,which indicates that dynamic membrane aeration produced a high k_(L)a by fine bubbles;a higher k_(L)a is more bene-ficial to aerobic fermentation.The starting time of continuous fermentation in the RDMAB was 20 h ear-lier than that in the CCAB,which greatly improved the biological process.During continuous fermentation,maintaining the same dissolved oxygen level and a constant dilution rate,the biomass accumulation and RNA concentration in the RDMAB were 9.71% and 11.15% higher than those in the CCAB,respectively.Finally,the dilution rate of RDMAB was 16.7% higher than that of CCAB during con-tinuous fermentation while maintaining the same air aeration.In summary,RDMAB is more suitable for continuous fermentation processes.Developing new aeration and structural geometry in airlift bioreac-tors to enhance k_(L)a and gas holdup is becoming increasingly important to improve bioprocesses in a bioreactor.

关键词： Airlift bioreactor Dynamic membrane Kinetic model Batch fermentation continuous fermentation

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Principles and Case Studies of Fed Batch fermentation and continuous fermentation

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Journal of Clinical and Nursing Research 2022年第2期6卷 99-104页

作者： Andrew Peng Yi Keystone Academy Beijing 101318China

Modern fermentation processes include a variety of fermentation methods,such as fed batch fermentation and continuous fermentation.This paper will focus on the principles and case studies of these two methods.Fed batch fermentation originates from fractionation fermentation with closed culture and adjustment of the pH value of the carbon source,to which the process of feeding the carbon source to the cell culture in a controlled manner has been added.This type of fermentation is more commonly used compared to the other.continuous fermentation is also a closed fermentation system,which can operate without restrictions by continuous or intermittent addition of fresh nutrient media to the fermenter;however,it is susceptible to contamination by stray bacteria and metabolic inconvenience.

关键词： Fed batch fermentation continuous fermentation fermentation process

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continuous Bioethanol Production Using Saccharomyces cerevisiae Cells Immobilized In Nata De Coco （Biocellulose）

Continuous Bioethanol Production Using Saccharomyces cerevis...

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2012 2nd International Conference on Environment and Industrial Innovation（ICEII 2012）

作者： Charlimagne M.Montealegre Emerson R.Dionisio Lawrence V.Sumera Jay R T.Adolacion Rizalinda L.De Leon Biochemical Engineering Laboratory Department of Chemical Engineering College of EngineeringUniversity of the Philipines Diliman Quezon City 1101 Philippines

The performance of Nata de coco （NDC） and Calcium alginate （CA） as an immobilization medium for Saccharomyces cerevisiae cells are compared in terms of production rate and conversion. S. cerevisiae cells are immobilized in NDC and CA beads using a cell suspension with an average approximate live cell density of 232.1288 ± 1.5387 cells/mL. The biocatalysts NDC and CA are charged into horizontal fermentation reactors. A centrifugal pump and manifold is used to control the flow rate to a desired flow rate of 9 mL/hr. Samples are collected every 12 hours and tested for ethanol by gas chromatography and glucose concentration by colorimetry. The average steady state effluent ethanol concentration, productivity and conversion in NDC are 5.093 % by volume, 52.329 mL/hr and 0.7779, respectively. One-way ANOVA showed that the immobilization medium has a significant effect on the parameters under consideration. T-test is further performed between NDC and CA biocatalysts which showed that effluent ethanol concentration, productivity and conversion of NDC and CA are statistically equal. The study showed that the NDC biocatalyst performs equally well in the conditions optimized for CA biocatalyst. The structural strength and cost effectiveness of Nata de Coco makes it a very promising immobilization medium for continuous bioethanol production.

关键词： Immobilization Biocellulose Calcium Alginate continuous fermentation Horizontal Reactor Baker’s Yeast

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