Continuous Bioethanol Production Using Saccharomyces cerevisiae Cells Immobilized In Nata De Coco （Biocellulose）
作者单位：Biochemical Engineering Laboratory Department of Chemical Engineering College of EngineeringUniversity of the Philipines Diliman Quezon City 1101 Philippines
会议名称：《2012 2nd International Conference on Environment and Industrial Innovation（ICEII 2012）》
会议日期：2012年
学科分类：081702[工学-化学工艺] 08[工学] 0817[工学-化学工程与技术]
关 键 词：Immobilization Biocellulose Calcium Alginate Continuous Fermentation Horizontal Reactor Baker’s Yeast
摘 要：The performance of Nata de coco （NDC） and Calcium alginate （CA） as an immobilization medium for Saccharomyces cerevisiae cells are compared in terms of production rate and conversion. S. cerevisiae cells are immobilized in NDC and CA beads using a cell suspension with an average approximate live cell density of 232.1288 ± 1.5387 cells/mL. The biocatalysts NDC and CA are charged into horizontal fermentation reactors. A centrifugal pump and manifold is used to control the flow rate to a desired flow rate of 9 mL/hr. Samples are collected every 12 hours and tested for ethanol by gas chromatography and glucose concentration by colorimetry. The average steady state effluent ethanol concentration, productivity and conversion in NDC are 5.093 % by volume, 52.329 mL/hr and 0.7779, respectively. One-way ANOVA showed that the immobilization medium has a significant effect on the parameters under consideration. T-test is further performed between NDC and CA biocatalysts which showed that effluent ethanol concentration, productivity and conversion of NDC and CA are statistically equal. The study showed that the NDC biocatalyst performs equally well in the conditions optimized for CA biocatalyst. The structural strength and cost effectiveness of Nata de Coco makes it a very promising immobilization medium for continuous bioethanol production.