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comparative proteomics analysis reveals the domesticated Lepista sordida primordium differentiation regulation mechanism and the subsequent different development patterns in the pileus and stipe

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Food Science and Human Wellness 2024年第5期13卷 3066-3082页

作者： Xiuqing Yang Meijie Liu Yanjiao Zhang Lizhong Guo Junqi Wang Sizhu Li Yan Zhang Xiaobo Li Lili Xu Hao Yu Shandong Provincial Key Laboratory of Applied Mycology School of Life SciencesQingdao Agricultural UniversityQingdao 266109China Shandong Agricultural Technology Extending Station Jinan 250100China Shandong Mushroom Industrial Technology Innovation Research Institute Jining 273500China

The wild Lepista sordida is a kind of precious and rare edible *** excellent strain of it by artificial domestication was obtained,which was high-yield and high in iron *** this study,high-throughput comparative proteomics was used to reveal the regulatory mechanism of its primordium differentiation in the early fruiting body *** mycelium before the primordium differentiation mainly expressed high levels of mitochondrial functional proteins and carbon dioxide concentration regulatory *** young mushrooms,the highly expressed proteins were mainly involved in cell component generation,cell proliferation,nitrogen compound metabolism,nucleotide metabolism,glutathione metabolism,and purine *** differential regulation patterns of pileus and stipe growth to maturity were also *** highly expressed proteins related to transcription,RNA splicing,the production of various organelles,DNA conformational change,nucleosome organization,protein processing,maturation and transport,and cell detoxification regulated the pileus development and *** proteins related to carbohydrate and energy metabolism,large amounts of obsolete cytoplasmic parts,nutrient deprivation,and external stimuli regulated the stipe development and *** CAZymes regulated nutrient absorption,morphogenesis,spore production,stress response,and other life activities at different growth and development stages.

关键词： Lepista sordida comparative proteomics Primordial differentiation Growth and development regulation Pileus Stipe

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comparative proteomics analysis of tea leaves exposed to subzero temperature:Molecular mechanism of freeze injury

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International Journal of Agricultural and Biological Engineering 2013年第4期6卷 27-34页

作者： Hu Yongguang Lu Yongzong Lu Jian Institute of Agricultural Engineering Jiangsu UniversityZhenjiang 212013JiangsuChina Institute of Life Sciences Jiangsu UniversityZhenjiang 212013JiangsuChina

Tea freeze injury is one of the most severe agro-meteorological disasters,which leads to sizable losses of tea production in *** freezing resistant ability of overwintering tea trees becomes weaker and weaker from early-spring to *** it decreases to critical temperature of-2℃or lower in the stage with one or two leaves,tea trees suffer from freeze injury and the yield or quality of spring tea production could decrease *** measurements have been taken to prevent such damage,the physiological and biochemical mechanism of how tea(Camellia Sinensis)plant response to freeze injury is still to be elucidated.A comparative proteomics analysis was made on tea leaves at the two-leaf *** differential image analysis showed 46 spots with density changes(29 spots increased and 17 spots decreased;p<0.01)in the freeze injury group compared with the control *** eight differential protein spots(p<0.01)with good resolution and relatively high abundance in MS were subjected to further protein *** them,all 17 up-regulated spots were collected whereas only six of the down-regulated spots were *** differentially expressed proteins including heat shock protein 70,oxygen-evolving enhancer protein,adenosine triphosphate synthase,S-adenosylmethionine synthetase and some enzymes involved in carbohydrate metabolism,were shown responsive to freeze *** results would greatly increase the comprehension of the molecular mechanism for freeze injury and provide a better decision making for freeze protection and control.

关键词： freeze injury molecular mechanism freeze protection comparative proteomics Camellia Sinensis mass spectrometry

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comparative proteomics in gastric cancer cell line BGC823 after ZNF139gene inhibited with RNA interference

Comparative Proteomics in gastric cancer cell line BGC823 af...

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第9届全国胃癌学术会议暨第二届阳光长城肿瘤学术会议

作者：范立侨李勇赵群檀碧波马连港刘羽王冬河北医科大学第四医院外三科

Background and aim To study changes of proteins after zinc finger protein 139（ZNF139） gene was inhibited in gastric cancer cell line BGC823,and to explore regulation mechanisms of ZNF139 to gastric cancer *** siRNA-specific ZNF139 was synthesized and transfected into gastric cancer cell line BGC823;2-D fluorescence difference gel electrophoresis（2-D DIGE） and liquid chromatography-mass spectrometry（LC-MS） were applied to screen,identify differentially expressed proteins in BGC823 cells before and after transfection,and function of these proteins was analyzed;Western blot method was applied to verify the identified *** ZNF139 expression in siRNA transfected cancer cell BGC823 was significantly decreased;before and after transfection 2-D DIGE was used to analyze and identify eight distinct differential protein spots were analyzed and identified in transfected and non- transfected BGC823 cells with2-D DIGE,of which seven were identified with LC-MS technology,including switches associated protein 70,far upstream element binding protein 1,heat shock protein 60,annexin A7,small ubiquitin-like modifier 1 activating enzyme（SUMO 1-activating enzyme）,chaperonin-containing tail-less complex protein 1 and annexin *** analysis was applied,and these proteins were found to be associated with proliferation,apoptosis,invasion,metastasis and adhesion of gastric cancer *** blot analysis confirmed that expressions of these proteins in BGC823were consistent with those in the proteomic *** ZNF139 gene may influence the biological behavior of gastric cancer cells in many ways by regulating multiple genes and protein expressions.

关键词： Gastric cancer Zinc finger protein 139 RNA interference comparative proteomics

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comparative proteomics studies on brown planthopper responding to host rice resistance

Comparative proteomics studies on brown planthopper respondi...

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2016年全国植物生物学大会

作者： Kaikai Zeng Siqi Wang Juanjuan Zhang Zhifan Yang Hubei Collaborative Innovation Center for Green Transformation of Bio-Resource College of Life SciencesHubei University

The brown planthopper(BPH,Nilaparvata lugens) is one of the most destructive pests of rice. comparative proteomics analyses of BPH will help to find new measures to control BPH. In this work,comparative proteomics analyses of the fourth instar nymphs of biotype I and II which infested the susceptible rice TN1 and the resistant rice Mudgo,were carried out by isobaric tag-based methodology for relative peptide quantification(iTRAQ) technique. The results revealed that 7385 protein fragments were detected,and 696 fragments were quantified. Total 201 proteins showed obviously different expression levels. These proteins were expressed differently between the two types of BPH,or the same type BPH feeding on the two rice varieties with different resistant levels. 27 proteins were selected to investigate the expression level of the corresponding genes by fluorescent quantitative real-time PCR. The results revealed that most of the genes have the same expression trends with their coding proteins. It is noteworthy that three genes(vitellogenin,fatty acid synthase,protein kinase) were expressed at an extremely different pattern,and probably involved in regulating biotype formation and/or the acute stress reaction. This study will help in intensifying the knowledge of the interaction mechanism of BPH and rice,including: (1) during the acute stress reaction of BPH to host rice resistance,the variation laws of the midgut proteomics,which will indirectly provide some important information about the resistance mechanism of rice;(2) the discrepancy between different biotype BPH midgut proteomics,which will provide some important information for division of biotype and elucidating the molecular mechanism of the formation of a special biotype.

关键词： brown planthopper(Nilaparvata lugens Hemiptera Delphacidae) biotype host rice resistance isobaric tag-based methodology for relative peptide quantification(iTRAQ) comparative proteomics

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comparative proteomics of Rice Starch Granules to Look for Candidate Proteins in Rice Starch Biosynthesis

Comparative Proteomics of Rice Starch Granules to Look for C...

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江苏省遗传学会2016年学术年会

作者：邢世海 Chunfang Zhao Yadong Zhang Lihui Zhou 王才林 Institute of Food Crops Jiangsu Academy of Agricultural SciencesJiangsu High Quality Rice Research and Development CenterNanjing Branch of China National Center for Rice Improvement

Starch is the most important food energy source in *** of the known enzymes involved in starch biosynthesis are partially or entirely granule-associated in the *** the proteome of rice starch granule is important for us to further understand the mechanisms underlying starch biosynthesis and starch granules packaging in rice *** look for new candidate proteins which may be involved in starch biosynthesis in rice endosperm,we developed a protocol to purified starch granules from mature rice endosperm and verified the quality of the starch granules by microscopy observations,I staining,and Western blot *** protocol we developed can effectively purify starch granules from mature endosperm in *** enrichment of the starch granules were verified by light and SEM microscopy,I staining,and western blot *** the purification method established,we purified total protein and proteins in starch granule in a japonica rice strain Wujing 13 and a semi-glutinous rice Guandong 194 to do 2-D gel electrophoresis *** found some spots difference between them in purified starch granules proteome while there’s no significant difference spot between them in the total proteome.2-D result showed by purification the different proteins between them in the starch granule had been enriched,and these different protein spots in gel will be cut and analysis by LC/MS identification to look for new candidate proteins may be in starch synthesis in rice.

关键词： rice:starch granules comparative proteomics 2-D gel electrophoresis starch biosynthesis

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comparative proteomics in gastric cancer cell line BGC823 after ZNF139 gene inhibited with RNA interference

Comparative Proteomics in gastric cancer cell line BGC823 af...

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第九届全国免疫学学术大会

作者： LI-QIAO FAN YONG LI QUN ZHAO BI-BO TAN LIAN-GANG MA YU LIU DONG WANG Department of General Surgery the Fourth Affiliated HospitalHebei Medical University

Objective:To study changes of proteins after zinc finger protein 139(ZNF139)gene was inhibited in gastric cancer cell line BGC823,and to explore regulation mechanisms of ZNF139 to gastric cancer ***:siRNA-specific ZNF139 was synthesized and transfected into gastric cancer cell line BGC823;2-D fluorescence difference gel electrophoresis(2-D DIGE)and liquid chromatography-mass spectrometry(LC-MS)were applied to screen,identify differentially expressed proteins in BGC823 cells before and after transfection,and function of these proteins was analyzed;Western blot method was applied to verify the identified ***:ZNF139 expression in siRNA transfected cancer cell BGC823 was significantly decreased;before and after transfection 2-D DIGE was used to analyze and identify eight distinct differential protein spots were analyzed and identified in transfected and non-transfected BGC823 cells with 2-D DIGE,of which seven were identified with LC-MS technology,including switches associated protein 70,far upstream element binding protein 1,heat shock protein 60,annexinA7,small ubiquitin-like modifier 1 activating enzyme(SUMO 1-activating enzyme),chaperonincontaining tail-less complex protein 1 and annexin *** analysis was applied,and these proteins were found to be associated with proliferation,apoptosis,invasion,metastasis and adhesion of gastric cancer *** blot analysis confirmed that expressions of these proteins in BGC823 were consistent with those in the proteomic ***:ZNF139 gene may influence the biological behavior of gastric cancer cells in many ways by regulating multiple genes and protein expressions.

关键词： Gastric cancer Zinc finger protein 139 RNA interference comparative proteomics

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comparative proteomics Analysis of the Sieve Tube from Rubber Tree(Hevea brasiliensis) Seedlings under Contrasting Ethylene Treatments

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Agricultural Science & Technology 2019年第2期20卷 18-27页

作者： DAI Long-jun QIN Yun-xia YANG Hong DENG Zhi SHI Min-jing LIU Hui TANG Chao-rong LI De-jun Key Laboratory of Biology and Genetic Resources of Rubber Tree Ministry of Agriculture and Rural AffairsState Key Laboratory Breeding Base of Cultivation and Physiology for Tropical CropsRubber Research InstituteChinese Academy of Tropical Agricultural SciencesHaikou 571101PRC

[Objective]To elucidate the role of ethylene(ET),a latex yield stimulant of the rubber tree,on the sieve tube(ST)transport efficiency of materials(especially sucrose)needed for natural rubber biosynthesis.[Method]Rubber tree seedlings were treated with ET solution or water which was used as a control on the bark,and latex samples and ST tissue samples were collected for proteomic analyses and latex sucrose content determination respectively.[Results]After ET treatment,the sucrose content of the latex was found significantly decreased.A total of 66 ethylene-responsive proteins(ERPs)were distinguished by two-dimensional gel electrophoresis(2-DE),and 54 were successfully identified by MALDI-TOF/TOF and database *** majority of these ERPs were involved in carbohydrate transport and metabolic processes in the ST.[Conclusion]Our findings suggest that the application of ET may increase the transport efficiency of the ST and that the application of ET promotes the consumption of energy and sucrose in the ST.

关键词： Sieve tube Rubber tree comparative proteomics Ethylene-responsive proteins Sucrose content

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Proteomic-based evidence for adult neurogenesis in birds and mammals as indicated from cerebrospinal fluid

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Neural Regeneration Research 2022年第12期17卷 2576-2581页

作者： Eleni Voukali Michal Vinkler Department of Zoology Faculty of ScienceCharles UniversityPragueCzech Republic

Adult neurogenesis is the life-long process of neural stem cell proliferation,differentiation into neurons,migration,and incorporation into the existing neuronal *** decades of research,it is now widely accepted that mammals and birds retain the capacity to regenerate neurons even after their subadult *** fluid participates in the regulation of the neurogenic niches of the vertebrate brain through signaling pathways not fully *** studies of cerebrospinal fluid have the potential to allow the in-depth characterization of its molecular *** studies help to delineate those pathways that are universally critical for the regulation of neurogenesis in *** this review,we performed literature-based data mining in studies using liquid chromatography-tandem mass spectroscopy that analyzed cerebrospinal fluid samples from healthy adult humans(Homo sapiens);mice(Mus musculus);sheep(Ovis aries);chickens(Gallus gallus);and two parrot species,the budgerigar(Melopsittacus undulatus)and cockatiel(Nymphicus hollandicus).We identified up to 911 proteins represented in cerebrospinal fluid,involved in various pathways regulating adult ***,only 196 proteins were common across humans,mice,and *** components involved in nervous system development,cell migration,and axonal guidance were commonly evident in all species investigated so *** bioinformatic analysis revealed that the universally over-represented pathways involved L1 cell adhesion molecule protein interactions,cell-adhesion molecules,signals regulating extracellular matrix remodeling,regulation of insulin growth factor signaling,axonal guidance,programmed cell death,immune signaling,and post-translational *** of the reported proteins are part of extracellular vesicles enriched in cerebrospinal ***,the information presently available is still highly fragmentary,and far more questions persist than

关键词： adult neurogenesis birds cerebrospinal fluid chicken comparative proteomics human mammals mass spectrometry parrots

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comparative Proteomic Analysis in Left Ventricular Remodeling following Myocardial Infarction in Rats

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Biomedical and Environmental Sciences 2012年第1期25卷 117-123页

作者： GU Hong Juan GAO Chang Bin GONG Jun Li LI Xiang Jun SUN Bo LI Xi Ning China-Japan Union Hospital of Jilin University Changchun 130021 Jilin China Changchun Municipal Central Hospital Changehun 130051 Jilin China Jilin University College of Pharmacy Changchun 130021 Jilin China

Objective Left ventricular remodeling （LVR） following myocardial infarction （MI） is a key pathophysiological process in which MI develops into heart failure. The exact mechanism of LVR remains unclear. We performed differential proteomic analysis on the myocardia of rats with LVR after MI, to explore the mechanism of ventricular remodeling after MI. Methods In the LVR group （n=12）, after the anterior descending coronary artery was ligated, the rats were fed for four weeks before the LVR models were established. Rats in the sham-operated group （n=11） underwent thread-drawing without ligation. The hemodynamic parameters, pathological findings, and proteomics were compared between the two groups. Results In the LVR group, the left ventricular end-diastolic pressure increased, the maximal left ventricular pressure increase/decrease ratio decreased significantly, and the left ventricular systolic pressure decreased. H-E staining and Masson staining of cardiac muscle tissues of the LVR group showed myocytolysis, disarray, and collagen proliferation. Twenty-one differentially expressed proteins were detected by proteomic analysis. We validated two proteins using western blot analysis. The differentially expressed proteins could be divided into six categories： energy metabolism-related proteins, cytoskeletal proteins, protein synthesis-related proteins, channel proteins, anti-oxidation- related proteins, and immune-related proteins. Conclusion These differentially expressed proteins might play key roles in LVR following M

关键词： comparative proteomics Liquid chromatography-mass spectrometry Left ventricular remodeling Myocardial infarction Western blot

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Ascorbate peroxidase 1 confers resistance to southern corn leaf blight in maize

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Journal of Integrative Plant Biology 2022年第6期64卷 1196-1211页

作者： Jinghua Zhang Xingmeng Jia Guan-Feng Wang Shijun Ma Shunxi Wang Qin Yang Xueyan Chen Yuqian Zhang Yajing Lyu Xiaoxu Wang Jiawei Shi Yangtao Zhao Yanhui Chen Liuji Wu National Key Laboratory of Wheat and Maize Crop Science Collaborative Innovation Center of Henan Grain CropsCollege of AgronomyHenan Agricultural UniversityZhengzhou 450002China The Key Laboratory of Plant Development and Environmental Adaptation Biologym Ministry of EducationSchool of Life SciencesShandong UniversityQingdao 266237China State Key Laboratory of Crop Stress Biology in Arid Areas College of AgronomyNorthwest A&F UniversityYangling 712100China School of Environmental and Rural Science University of New EnglandArmidale 2351NSWAustralia

Southern corn leaf blight (SCLB), caused by Bipolarismaydis, is one of the most devastatingdiseases affecting maize production. However,only one SLCB resistance gene, conferring partialresistance, is currently known, underscoring theimportance of isolating new SCLB resistancerelatedgenes. Here, we performed a comparativeproteomic analysis and identified 258 proteinsshowing differential abundance during the maizeresponse to B. maydis. These proteins included anascorbate peroxidase (Zea mays ascorbate peroxidase1 (ZmAPX1)) encoded by a gene locatedwithin the mapping interval of a previously identifiedquantitative trait locus associated with SCLBresistance. ZmAPX1 overexpression resulted inlower H_(2)O_(2) accumulation and enhanced resistanceagainst B. maydis. Jasmonic acid (JA)contents and transcript levels for JA biosynthesisand responsive genes increased in ZmAPX1-overexpressing plants infected with B. maydis,whereas Zmapx1 mutants showed the oppositeeffects. We further determined that low levels of H_(2)O_(2) are accompanied by an accumulation of JAthat enhances SCLB resistance. These resultsdemonstrate that ZmAPX1 positively regulatesSCLB resistance by decreasing H_(2)O_(2) accumulationand activating the JA-mediated defensesignaling pathway. This study identified ZmAPX1as a potentially useful gene for increasing SCLBresistance. Furthermore, the generated datamay be relevant for clarifying the functions ofplant APXs.

关键词： ascorbate peroxidase comparative proteomics disease resistance jasmonic acid maize southern corn leaf blight

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