检索结果-南通市图书馆

Soil Ecology Letters 2023年第1期5卷 142-150页

作者： Yixin Sun Xiaofang Du Yingbin Li Xu Han Shuai Fang Stefan Geisen Qi Li CAS Key Laboratory of Forest Ecology Management Institute of Applied EcologyChinese Academy of SciencesShenyang 110016China University of Chinese Academy of Sciences Beijing 100049China Laboratory of Nematology Wageningen UniversityWageningenThe Netherlands

High-throughput sequencing technology is increasingly used in the study of nematode biodiversity.However,the annotation difference of commonly used primers and reference databases on nematode community is still unclear.We compared two pairs of primers(3NDf/C_1132rmod,NF1F/18Sr2bR)and three databases(NT_V20200604,SILVA138/18s Eukaryota and PR2_v4.5 databases)on the determination of nematode community from four different vegetation types in Changbai Mountain,including mixed broadleaf-conifer forest,dark coniferous forest,betula ermanii Cham and alpine tundra.Our results showed that the selection of different primers and databases influenced the annotation of nematode taxa,but the diversity of nematode community showed consistent pattern among different vegetation types.Our findings emphasize that it is necessary to select appropriate primer and database according to the target taxonomic level.The difference in primers will affect the result of nematode taxa at different classification levels,so sequencing analysis cannot be used for comparison with studies using different primers.In terms of annotation effect in this study,3NDf/C_1132rmod primers with NT_v20200604 database could provide more information than other combinations at the genus or species levels.

关键词： Soil nematodes Primer Database High-throughput sequencing community composition

Warming intensified the effects of nitrogen addition on N_(2)O emissions from alpine meadow in the northern Qinghai-Tibet Plateau

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Advances in Climate Change Research 2024年第1期15卷 101-112页

作者： Ming-Jie LI Yi-Qing GE Hasbagan GANJURJAV Guo-Zheng HU Hong-Bao WU Jun YAN Shi-Cheng HE Qing-Zhu GAO Institute of Environment and Sustainable Development in Agriculture Chinese Academy of Agricultural SciencesBeijing 100081China National Agricultural Experimental Station for Agricultural Environment Nagqu 852100China Ecology and Nature Conservation Institute Chinese Academy of ForestryBeijing 100091China College of Resource and Environment Anhui Science and Technology UniversityFengyang 233100China Nagqu Agriculture Animal Husbandryand Prataculture Science and Technology Research and Extension CenterNagqu 852100China Tongliao Experimental Middle School Tongliao 028000China

Warming and nitrogen(N)addition may impact soil nitrous oxide(N_(2)O)emissions,but the relationship between plant community composition and soil microbial activities remains unclear.For a two-year field study in the Qinghai-Tibet Plateau,open-top chambers were used to quantify the effects of warming,N-addition,and their interactions on N_(2)O emissions.We found that the N-addition greatly increased N_(2)O emissions by 77.4%in 2018 when compared to the control group.In contrast,warming showed little effect on N_(2)0 emissions but did increase the activity of enzymes associated with soil nitrification and denitrification.A combined effect of warming and N-addition of resulted in 208.6%(2018)and 90.8%(2019)increase in N_(2)0 emissions,respectively,compared to the individual treatments of warming or N-addition.Global warming in alpine meadows is causally linked to increased legume biomass which is further intensified with the N-addition.Intensified legume biomass(p<0.05),soil moisture(p<0.001)and enzyme activity(p<0.001)had a positive effect on N_(2)0 emissions,while diminished microbial carbon/nitrogen(MBC/MBN)(p<0.05)correlated with reduced N_(2)O emissions.Final results indicated that N-addition has a positive effect on N_(2)O emissions,and the addition of warming further intensifies this effect.The increased dominance of legumes and microbial N content contributes to this effect.These outcomes suggest that warming and atmospheric N deposition can stimulate N_(2)O emissions of alpine meadows inthefuture.

关键词： Alpine meadow community composition Nitrogen addition Nitrous oxide flux Qinghai-Tibet Plateau Warming

Response of community composition and biomass of submerged macrophytes to variation in underwater light, wind and trophic status in a large eutrophic shallow lake

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Environmental Sciences 2021年第5期33卷 298-310页

作者： Baili Dong Yongqiang Zhou Erik Jeppesen Kun Shi Boqiang Qin Taihu Laboratory for Lake Ecosystem Research State Key Laboratory of Lake Science and EnvironmentNanjing Institute of Geography and LimnologyChinese Academy of SciencesNanjing 210008China University of Chinese Academy of Sciences Beijing 100049China Department of Bioscience Aarhus UniversityVejlsovej 258600 SilkeborgDenmark Sino-Danish Centre for Education and Research(SDC) University of Chinese Academy of SciencesBeijing 100049China Limnology Laboratory Department of Biological Sciences and Centre for Ecosystem Research and ImplementationMiddle East Technical UniversityAnkaraTurkey

Light climate is of key importance for the growth, community composition of submerged macrophytes in lakes and, they, in turn, are affected by lake depth and the degree of eutrophication. To test the relationships between submerged macrophyte presence and the ratio of Secchi disk depth(SDD) to water depth, i.e. SDD/depth, nutrients and wind, we conducted an extensive sampling campaign in a macrophyte-dominated area of the eastern region( n = 36) in 2016 in Lake Taihu, China, and combined the data gathered with results from extensive physico-chemical monitoring data from the entire lake. We confirmed that SDD/Depth is the primary factor controlling the community composition of macrophytes and showed that plant abundance increased with increasing SDD/Depth ratio( p 0.4 ensured growth of submerged macrophytes. Total phosphorus and total nitrogen also influenced the growth and community composition of macrophytes( p 0.05). The key to restore the macrophyte beds in the lake is to reduce the nutrient loading. A decrease of the water level may contribute as well in the shallow bays but will not bring plants back in the main part of the lake. As the tolerance of shade and nutrients varied among the species studied, this should be taken into account in the restoration of lakes by addition of plants.

关键词： Underwater light climate Water depth community composition Macrophyte Eutrophication

Estimating areal carbon fixation of intertidal macroalgal community based on composition dynamics and laboratory measurements

在线全文

学校读者我要写书评

暂无评论

Journal of Oceanology and Limnology 2019年第1期37卷 93-101页

作者： DU Guoying ZHAO Ezi LIU Chunrong TANG Xianghai Key Laboratory of Marine Genetics and Breeding Chinese Ministry of Education College of Marine Life Sciences Ocean University of China

The community dynamics and potential carbon ?xation of intertidal macroalgae were investigated monthly from April 2014 to April 2015 in the northwest coast of Yellow Sea. Seasonal variations in biomass and carbon ?xation were presented and showed close relationship with community structure.The carbon ?xation rate ranged from 0.48±0.13 mg C/(g FW ·d) to 4.35±0.12 mg C/(g FW ·d). Sargassum thunbergii, Chondrus ocellatus and Ulva intestinalis were three most in?uential species which contributed27%, 21.9% and 18.5% variation of carbon ?xation rate, respectively. Standing carbon stocks ranged from7.52 g C/m^2 to 41.31 g C/m^2, and estimated carbon stocks varied from 11.77 g C/m 2 to 96.49 g C/m^2. The larger dif ference between estimated and standing carbon stocks implied that more ?xed carbon was exported from the community in summer and autumn than in winter. This study suggested that intertidal macroalgal community could provide a potential function in carbon ?xation of coastal ecosystem.

关键词： carbon fxation carbon stock community composition intertidal macroalgae Yellow Sea

The bacterial diversity and community composition altered in the oxygen minimum zone of the Tropical Western Pacific Ocean

在线全文

学校读者我要写书评

暂无评论

Journal of Oceanology and Limnology 2021年第5期39卷 1690-1704页

作者： Qiqi SUN Jinming SONG Xuegang LI Huamao YUAN Qidong WANG Shandong Peanut Research Institute Qingdao 266100China Key Laboratory of Marine Ecology and Environmental Sciences Institute ofOceanologyChinese Academy of SciencesQingdao 266071China Laboratory for Marine Ecology and Environmental Science Pilot NationalLaboratory for Marine Science and Technology(Qingdao)Qingdao 266237China University of Chinese Academy of Sciences Beijing 100049China Center for Ocean Mega-Science Chinese Academy of SciencesQingdao266071China

The oxygen minimum zones(OMZs)are globally expanding,yet the variation pattern of microbial communities related to dissolved oxygen levels remain unclear.Spatial variability of bacterial diversity and community composition(repre sented by 16 S rRNA)of six stations was investigated within the water column in the seamount area of Tropical Western Pacific Ocean(TWPO)in May 2019.The seawater has dissolved oxygen(DO)concentration of 3.01-6.68 mg/L and the core of the oxygen minimum zones was located between the depths of 650 m and 1750 m.The bacterial alpha-diversity showed unimodal pattern with the decreasing DO with depths and peaked in the upper oxycline(UO)of OMZs.The bacterial community structure of the mixed layer(ML)and the bottom layer clustered and separated from each other,while those of UO and the OMZ core(OM)clustered and overlapped.Overall,bacterial community composition transitioned from being Alphaproteobacteria and Gammaproteobacteria-dominant in ML to being Gammaproteobacteria and Nitrososphaeria/Deltaproteobacteria-dominant in UO and OM,and then changed to being Clostridia and unidentified Actinobacteria-dominant in the bottom layer.Moreover,both bacterial alpha-diversity and the abundant classes fitted varying sectioned functions with DO.The DO solely explained 40.37%of the variation of bacterial community composition among layers(Pcommunity composition,and help to clarify the potential responses of microbes and their involved biogeochemical processes to the expansion and intensification of OMZs.

关键词： bacterial diversity community composition oxygen minimum zones(OMZs) dissolved oxygen Tropical Western Pacific Ocean(TWPO)

Effects of long-term straw incorporation on nematode community composition and metabolic footprint in a rice–wheat cropping system

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2021年第8期20卷 2265-2276页

作者： CHEN Yun-feng XIA Xian-ge HU Cheng LIU Dong-hai QIAO Yan LI Shuang-lai FAN Xian-peng Key Laboratory of Fertilization from Agricultural Wastes Ministry of Agriculture and Rural Affairs/Institute of Plant Protection and Soil FertilizerHubei Academy of Agricultural SciencesWuhan 430064P.R.China

Soil nematode communities can provide valuable information about the structure and functions of soil food webs,and are sensitive to agricultural practices,including short-term straw incorporation.However,currently,such effects under longterm straw incorporation conditions at different fertility levels are largely unknown.Thus,we conducted a 13-year ongoing experiment to evaluate the effects of long-term straw incorporation on the structure and functions of the soil food web in low and high fertility soils through analyzing its effects on nematode communities,food web indices and metabolic footprints.Four treatments were included:straw removal(–S)under non-fertilized(–NPK)or fertilized(+NPK)conditions;and straw incorporation(+S)under–NPK or+NPK conditions.Soil samples from a 0–20 cm depth layer were collected when wheat and rice were harvested.Compared with straw removal,straw incorporation increased the abundances of total nematodes,bacterivores,plant-parasites and omnivores-predators,as well the relative abundances of omnivores-predators with increases of 73.06,89.29,95.31,238.98,and 114.61%in–NPK soils and 16.23,2.23,19.01,141.38,and 90.23%in+NPK soils,respectively.Regardless of sampling times and fertilization effects,straw incorporation increased the diversity and community stability of nematodes,as indicated by the Shannon-Weaver diversity index and maturity index.Enrichment and structure index did not show significant responses to straw incorporation,but a slight increase was observed in the structure index.The analysis of nematode metabolic footprints showed that straw incorporation increased the plant-parasite footprint and structure footprint by 97.27 and 305.39%in–NPK soils and by 11.29 and 149.56%in+NPK soils,but did not significantly influence enrichment,bacterivore and fungivore footprints.In conclusion,long-term straw incorporation,particularly under a low fertility level,favored the soil nematodes and regulated the soil food web mainly via a top-down effect.

关键词： soil nematodes community composition metabolic footprint straw incorporation rice-wheat cropping system top-down effect

Immediate and legacy effects of snow exclusion on soil fungal diversity and community composition

在线全文

学校读者我要写书评

暂无评论

Forest Ecosystems 2021年第2期8卷 292-302页

作者： Li Zhang Yuzhi Ren Kaijun Yang Zhijie Li Bo Tan Yang Liu Han Li Chengming You Sining Liu Lixia Wang Rui Yin Jian Zhang Zhenfeng Xu Forestry Ecological Engineering in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province&National Forestry and Grassland Administration Key Laboratory of Forest Resources Conservation and Ecological Safety on the Upper Reaches of the Yangtze River&Rainy Area of West China Plantation Ecosystem Permanent Scientific Research Base Institute of Ecology&ForestrySichuan Agricultural UniversityChengdu 611130China Global Ecology Unit CREAF-CSIC-UAB CSIC08193 BarcelonaCataloniaSpain Forschungszentrum Jülich GmbH Agrosphere(IBG-3)JülichGermany Helmholtz-Centre for Environmental Research-UFZ Department of Community EcologyTheodor-Lieser-Strasse 406110 Halle(Saale)Germany

Background:Soil fungi play crucial roles in ecosystem functions.However,how snow cover change associated with winter warming affects soil fungal communities remains unclear in the Tibetan forest.Methods:We conducted a snow manipulation experiment to explore immediate and legacy effects of snow exclusion on soil fungal community diversity and composition in a spruce forest on the eastern Tibetan Plateau.Soil fungal communities were performed by the high throughput sequencing of gene-fragments.Results:Ascomycota and Basidiomycota were the two dominant fungal phyla and Archaeorhizomyces,Aspergillus and Amanita were the three most common genera across seasons and snow manipulations.Snow exclusion did not affect the diversity and structure of soil fungal community in both snow-covered and snow-free seasons.However,the relative abundance of some fungal communities was different among seasons.Soil fungal groups were correlated with environmental factors(i.e.,temperature and moisture)and soil biochemical variables(i.e.,ammonium and enzyme).Conclusions:These results suggest that the season-driven variations had stronger impacts on soil fungal community than short-term snow cover change.Such findings may have important implications for soil microbial processes in Tibetan forests experiencing significant decreases in snowfall.

关键词： Winter climate change Snow cover Fungi community diversity community composition Illumina sequencing

When nets meet environmental DNA metabarcoding:integrative approach to unveil invertebrate community patterns of hypersaline lakes

在线全文

学校读者我要写书评

暂无评论

Journal of Oceanology and Limnology 2023年第4期41卷 1331-1340页

作者： Matthew A.CAMPBELL Alex LAINI Nicole E.WHITE Morten E.ALLENTOFT Mattia SACCÒ Trace and Environmental DNA(TrEnD)Laboratory School of Molecular and Life SciencesCurtin UniversityPerth 6102WAAustralia Department of Life Sciences and Systems Biology University of TurinTurin 10124Italy Subterranean Research and Groundwater Ecology(SuRGE)Group Trace and Environmental DNA(TrEnD)LaboratorySchool of Molecular and Life SciencesCurtin UniversityPerth 6102WAAustralia Lundbeck Foundation GeoGenetics Centre Globe InstituteUniversity of CopenhagenCopenhagen 1353Denmark

Saline and hypersaline wetlands account for almost half of the volume of inland water globally.They provide pivotal habitat for a vast range of species,including crucial ecosystem services for humans such as carbon sink storage and extractive resource reservoirs.Despite their importance,effective ecological assessment is in its infancy compared to current conventional surveys carried out in freshwater ecosystems.The integration of environmental DNA(eDNA)analysis and traditional techniques has the potential to transform biomonitoring processes,particularly in remote and understudied saline environments.In this context,this preliminary study aims to explore the potential of eDNA coupled with conventional approaches by targeting five hypersaline lakes at Rottnest Island(Wadjemup)in Western Australia.We focused on the invertebrate community,a widely accepted key ecological indicator to assess the conservational status in rivers and lakes.The combination of metabarcoding with morphology-based taxonomic analysis described 16 taxa belonging to the orders Anostraca,Diptera,Isopoda,and Coleoptera.DNA-based diversity assessment revealed more taxa at higher taxonomic resolution than the morphology-based taxonomic analysis.However,certain taxa(i.e.,Ephydridae,Stratyiomidae,Ceratopogonidae)were only identified via net surveying.Overall,our results indicate that great potential resides in combining conventional net-based surveys with novel eDNA approaches in saline and hypersaline lakes.Indeed,urgent and effective conservational frameworks are required to contrast the enormous pressure that these ecosystems are increasingly facing.Further investigations at larger spatial temporal scales will allow consolidation of robust,reliable,and affordable biomonitoring frameworks in the underexplored world of saline wetlands.

关键词： macroinvertebrate hypersaline e nvironmental DNA(eDNA) conservation ecological survey community composition

Microbial responses to the use of NaClO in sediment treatment

在线全文

学校读者我要写书评

暂无评论

Frontiers of Environmental Science & Engineering 2022年第2期16卷 25-38页

作者： Kun Li Tingming Ye Wang Zhang Jianfeng Peng Yaohui Bai Weixiao Qi Huijuan Liu Key Laboratory of Drinking Water Science and Technology Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085China Center for Water and Ecology State Key Joint Laboratory of Environment Simulation and Pollution ControlSchool of EnvironmentTsinghua UniversityBeijing 100084China University of Chinese Academy of Sciences Beijing 100049China China University of Mining&Technology Beijing 100083China

Chlorine is often used in algal removal and deodorization of landscape waters,and occasionally used as an emergency treatment of heavily polluted sediments.However,the ecological impact of this practice has not been fully studied and recognized.In this study,NaClO at 0.1 mmol/g based on dry weight sediment was evenly mixed into the polluted sediment,and then the sediment was incubated for 150 days to evaluate its microbial effect.Results showed that NaClO addition enhanced the release of TOC,TN,Cr and Cu from the sediment.The microbial richness in the examined sediment decreased continuously,and the Chao1 index declined from 4241 to 2731,in 150 days.The microbial community composition was also changed.The abundance of Proteobacteria and Bacteroidetes increased to 54.8%and 4.2%within 7 days compared to the control,and linear discriminant analysis(LDA)showed gram-negative bacteria and aerobic bacteria enriched after chlorination.The functional prediction with PICRUSt2 showed the functions of the microbial community underwent major adjustments,and the metabolic-related functions such as carbon metabolism,including pyruvate and methane metabolisms were significantly inhibited;besides,15 out of 22 analyzed key enzymes involved in C cycling and 6 out of 12 key enzymes or genes involved in N cycling were strongly impacted,and the enzymes and genes involved in carbon degradation and denitrification showed remarkable downregulation.It can be concluded that chlorination posed a seriously adverse effect on microbial community structure and function.This study deepens the understanding of the ecological effects of applying chlorine for environmental remediation.

关键词： Sediment chlorination Substance mobility Microbial response community composition Function

Impacts of backwashing on micropollutant removal and associated microbial assembly processes in sand filters

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Frontiers of Environmental Science & Engineering 2023年第3期17卷 87-92页

作者： Donglin Wang Jie Zhou Hui Lin Junwen Chen Jing Qi Yaohui Bai Jiuhui Qu Key Laboratory of Drinking Water Science and Technology Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085China University of Chinese Academy of Sciences Beijing 100049China Center for Water and Ecology State Key Joint Laboratory of Environment Simulation and Pollution ControlSchool of EnvironmentTsinghua UniversityBeijing 100084China

Backwashing is crucial for preventing clogging of sand filters.However,few studies have investigated the effect of backwashing on micropollutant removal and the dynamic changes in the microbial community in sand filters.Here,we used a series of manganese and quartz sand filters under empty bed contact times(EBCTs)of 2 h and 4 h to explore variations in micropollutant degradation and temporal dynamics of the microbial community after backwashing.The results showed that the removal efficiencies of caffeine,sulfamethoxazole,sulfadiazine,trimethoprim,atrazine,and active biomass recovered within 2 d after backwashing in both types of sand filters at 2-h EBCT,but the recovery of sulfadiazine and trimethoprim was not observed at 4-h EBCT.Moreover,the removal efficiency of atenolol increased after backwashing in the manganese sand filters,whereas maintained almost complete removal efficiency in the quartz sand filters at both EBCTs.Pearson correlation analysis indicated that microbial community composition gradually recovered to the pre-backwashing level(R increased from 0.53 to 0.97)at 2-h EBCT,but shifted at 4-h EBCT(Rcompositions of the recovered,depleted,and improved groups of microbes were distinguished by applying hierarchical clustering to the differentially abundant amplicon sequence variants.The cumulative relative abundance of recovered microbes at 2-h EBCT was 82.76%±0.43%and 46.82%±4.34%in the manganese and quartz sand filters,respectively.In contrast,at 4-h EBCT,the recovered microbes dropped to 15.55%–25.69%in both types of sand filters.

关键词： Sand filter Backwashing Recovery Micropollutants community composition