When nets meet environmental DNA metabarcoding:integrative approach to unveil invertebrate community patterns of hypersaline lakes

作     者：Matthew A.CAMPBELL Alex LAINI Nicole E.WHITE Morten E.ALLENTOFT Mattia SACCÒ Matthew A.CAMPBELL;Alex LAINI;Nicole E.WHITE;Morten E.ALLENTOFT;Mattia SACCò

作者机构：Trace and Environmental DNA(TrEnD)LaboratorySchool of Molecular and Life SciencesCurtin UniversityPerth 6102WAAustralia Department of Life Sciences and Systems BiologyUniversity of TurinTurin 10124Italy Subterranean Research and Groundwater Ecology(SuRGE)GroupTrace and Environmental DNA(TrEnD)LaboratorySchool of Molecular and Life SciencesCurtin UniversityPerth 6102WAAustralia Lundbeck Foundation GeoGenetics CentreGlobe InstituteUniversity of CopenhagenCopenhagen 1353Denmark 

出 版 物：《Journal of Oceanology and Limnology》 (海洋湖沼学报（英文）)

年 卷 期：2023年第41卷第4期

页      面：1331-1340页

核心收录：

学科分类：07[理学] 0713[理学-生态学] 

基　　金：Supported by the Curtin-BHP alliance within the framework of the“eDNA for Global Environment Studies(eDGES)”program 

主　　题：macroinvertebrate hypersaline e nvironmental DNA(eDNA) conservation ecological survey community composition 

摘      要：Saline and hypersaline wetlands account for almost half of the volume of inland water *** provide pivotal habitat for a vast range of species,including crucial ecosystem services for humans such as carbon sink storage and extractive resource *** their importance,effective ecological assessment is in its infancy compared to current conventional surveys carried out in freshwater *** integration of environmental DNA(eDNA)analysis and traditional techniques has the potential to transform biomonitoring processes,particularly in remote and understudied saline *** this context,this preliminary study aims to explore the potential of eDNA coupled with conventional approaches by targeting five hypersaline lakes at Rottnest Island(Wadjemup)in Western *** focused on the invertebrate community,a widely accepted key ecological indicator to assess the conservational status in rivers and *** combination of metabarcoding with morphology-based taxonomic analysis described 16 taxa belonging to the orders Anostraca,Diptera,Isopoda,and ***-based diversity assessment revealed more taxa at higher taxonomic resolution than the morphology-based taxonomic ***,certain taxa(i.e.,Ephydridae,Stratyiomidae,Ceratopogonidae)were only identified via net ***,our results indicate that great potential resides in combining conventional net-based surveys with novel eDNA approaches in saline and hypersaline ***,urgent and effective conservational frameworks are required to contrast the enormous pressure that these ecosystems are increasingly *** investigations at larger spatial temporal scales will allow consolidation of robust,reliable,and affordable biomonitoring frameworks in the underexplored world of saline wetlands.