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OsNAC5 orchestrates OsABI5 to fine-tune cold tolerance in rice

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Journal of Integrative Plant Biology 2024年第4期66卷 660-682页

作者： Ruiqing Li Yue Song Xueqiang Wang Chenfan Zheng Bo Liu Huali Zhang Jian Ke Xuejing Wu Liquan Wu Ruifang Yang Meng Jiang College of Agronomy Anhui Agricultural UniversityHefei 230036China Hainan Institute Yazhou Bay Sci-Tech CityZhejiang UniversitySanya 572025China National Key Laboratory of Rice Biology Advanced Seed InstituteZhejiang UniversityHangzhou 311225China State Key Laboratory of Rice Biology and Chinese National Center for Rice Improvement China National Rice Research InstituteHangzhou 311401China Key Laboratory of Germplasm Innovation and Genetic Improvement of Grain and Oil Crops(Co-Construction by Ministry and Province) Ministry of Agriculture and Rural AffairsCrop Breeding and Cultivation Research InstituteShanghai Academy of Agricultural SciencesShanghai 201106China

Due to its tropical origins,rice(Oryza sativa)is susceptible to cold stress,which poses severe threats to ***5,a NAC-type transcription factor,participates in the cold stress response of rice,but the detailed mechanisms remain poorly ***,we demonstrate that OsNAC5 positively regulates cold tolerance at germination and in seedlings by directly activating the expression of ABSCISIC ACID INSENSITIVE 5(OsABI5).Haplotype analysis indicated that single nucleotide polymorphisms in a NAC-binding site in the OsABI5 promoter are strongly associated with cold ***5 also enhanced OsABI5 stability,thus regulating the expression of cold-responsive(COR)genes,enabling fine-tuned control of OsABI5 action for rapid,precise plant responses to cold *** affinity purification sequencing coupled with transcriptome deep sequencing identified several OsABI5 target genes involved in COR expression,including DEHYDRATION-RESPONSIVE ELEMENT BINDING FACTOR 1A(OsDREB1A),OsMYB20,and PEROXIDASE 70(OsPRX70).In vivo and in vitro analyses suggested that OsABI5 positively regulates COR gene transcription,with marked COR upregulation in OsNAC5-overexpressing lines and downregulation in osnac5 and/or osabi5 knockout *** study extends our understanding of cold tolerance regulation via OsNAC5 through the OsABI5-CORs transcription module,which may be used to ameliorate cold tolerance in rice via advanced breeding.

关键词： ABA signaling ABI5 cold tolerance DREB NAC5 rice

Effects of Silicon Formulations on cold tolerance of Rice Seedlings

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Journal of Northeast Agricultural University(English Edition) 2024年第2期31卷 43-52页

作者： Ren Hongyu Wang Wenbo Li Xuecong Li Shuai Wen Yahuan Zhang Xingwen College of Resources and Environmental Sciences Northeast Agricultural UniversityHarbin 150030China College of Chemistry and Chemical Engineering Harbin Institute of TechnologyHarbin 150001China

To investigate the effects of silicon formulations on the cold tolerance of rice seedlings,Song Japonica 16(not cold tolerant)and Dongnong 427(cold tolerant)rice varieties were used as test materials and four different types of silicon formulations,Si-50-G,Si-60-G,Si-T-G,and Si-E-G,were applied as foliar sprays at the seedling stage,and a control group CK(equal amount of distilled water)was set *** week after the first silicon spray,two types of rice were subjected to low-temperature stress treatments at day/night temperatures of 12℃/10℃for 2,4,6,and 8 *** effects of different silicon formulations on the chlorophyll,proline(Pro)and soluble sugar contents as well as superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT)activities of rice seedlings under low-temperature stress were compared to find out the effects of silicon formulations on the cold tolerance of rice *** results showed that silicon formulations could significantly increase the chlorophyll content of rice seedling leaves,with Si-50-G being the most effective,with a significant increment of 40.17%compared to the CK at 2 days of low *** silicon formulations significantly increased the proline content and soluble sugar content of rice leaves at low temperature for 4-8 *** Song Japonica 16,the most significant increment in leaf POD activity was observed in Si-E-G treatment at 2,4 and 8 days of temperature stress,with 73.58%,20.95%and 217.24%increases compared to the CK,*** 4 and 6 days of temperature stress,the most significant increase in CAT activity was observed in Si-E-G treatment,with 25.70%and 75.78%increases compared to the CK,*** Dongnong 427,the Si-60-G treatment showed the highest increase in leaf SOD activity for 4 and 8 days of temperature stress,with significant increases of 58.15%and 82.76%compared to the CK,respectively,and the Si-E-G treatment showed the highest increase in leaf POD activity for 2 and 8 days of temperature st

关键词： silicon formulation rice seedling stage cold tolerance physiological indicator

Heterogeneous expression of stearoyl-acyl carrier protein desaturase genes SAD1 and SAD2 from Linum usitatissimum enhances seed oleic acid accumulation and seedling cold and drought tolerance in Brassica napus

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Journal of Integrative Agriculture 2024年第6期23卷 1864-1878页

作者： Jianjun Wang Yanan Shao Xin Yang Chi Zhang Yuan Guo Zijin Liu Mingxun Chen State Key Laboratory of Crop Stress Biology for Arid Areas/National Yangling Agricultural Biotechnology&Breeding Center/Shaanxi Key Laboratory of Crop Heterosis/College of Agronomy Northwest A&F UniversityYangling 712100China

Flax(Linum usitatissimum L.)is a versatile crop and its seeds are a major source of unsaturated fatty ***-acyl carrier protein desaturase(SAD)is a dehydrogenase enzyme that plays a key role in oleic acid biosynthesis as well as responses to biotic and abiotic ***,the function of SAD orthologs from *** has not been ***,we found that two LuSAD genes,LuSAD1 and LuSAD2,are present in the genome of *** cultivar‘Longya 10’.Heterogeneous expression of either LuSAD1 or LuSAD2 in Arabidopsis thaliana resulted in higher contents of total fatty acids and oleic acid in the ***,ectopic expression of LuSAD2 in *** caused altered plant ***,the overexpression of either LuSAD1 or LuSAD2 in Brassica napus also resulted in increased contents of total fatty acids and oleic acid in the ***,we demonstrated that either LuSAD1 or LuSAD2 enhances seedling resistance to cold and drought stresses by improving antioxidant enzyme activity and nonenzymatic antioxidant levels,as well as reducing membrane *** findings not only broaden our knowledge of the LuSAD functions in plants,but also offer promising targets for improving the quantity and quality of oil,and the abiotic stress tolerance of oil-producing crops,through molecular manipulation.

关键词： LuSAD oleic acid cold tolerance drought tolerance Linum usitatissimum Brassica napus

MdWRKY40is directly promotes anthocyanin accumulation and blocks MdMYB15L, the repressor of MdCBF2, which improves cold tolerance in apple

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Journal of Integrative Agriculture 2023年第6期22卷 1704-1719页

作者： XU Peng-yue XU Li XU Hai-feng HE Xiao-wen HE Ping CHANG Yuan-sheng WANG Sen ZHENG Wen-yan WANG Chuan-zeng CHEN Xin LI Lin-guang WANG Hai-bo Shandong Institute of Pomology Shandong Academy of Agricultural SciencesTai’an 271000P.R.China Modern Agriculture Research Institute of Yellow River Delta Shandong Academy of Agricultural SciencesDongying 257000P.R.China

cold stress is an important factor that limits apple production. In this study, we examined the tissue-cultured plantlets of apple rootstocks ‘M9T337' and ‘60-160', which are resistant and sensitive to cold stress, respectively. The enriched pathways of differentially expressed genes(DEGs) and physiological changes in ‘M9T337' and ‘60-160' plantlets were clearly different after cold stress(1°C) treatment for 48 h, suggesting that they have differential responses to cold stress. The differential expression of WRKY transcription factors in the two plantlets showed that MdWRKY40is and MdWRKY48 are potential regulators of cold tolerance. When we overexpressed MdWRKY40is and MdWRKY48in apple calli, the overexpression of MdWRKY48 had no significant effect on the callus, while MdWRKY40is overexpression promoted anthocyanin accumulation, increased callus cold tolerance, and promoted the expression of anthocyanin structural gene MdDFR and cold-signaling core gene MdCBF2. Yeast one-hybrid screening and electrophoretic mobility shift assays showed that MdWRKY40is could only bind to the MdDFR promoter. Yeast twohybrid screening and bimolecular fluorescence complementation showed that MdWRKY40is interacts with the CBF2inhibitor MdMYB15L through the leucine zipper(LZ). When the LZ of MdWRMY40is was knocked out, MdWRKY40is overexpression in the callus did not affect MdCBF2 expression or callus cold tolerance, indicating that MdWRKY40is acts in the cold signaling pathway by interacting with MdMYB15L. In summary, MdWRKY40is can directly bind to the MdDFR promoter in order to promote anthocyanin accumulation, and it can also interact with MdMYB15L to interfere with its inhibitory effect on MdCBF2, indirectly promoting MdCBF2 expression, and thereby improving cold *** results provide a new perspective for the cold-resistance mechanism of apple rootstocks and a molecular basis for the screening of cold-resistant rootstocks.

关键词： MdWRKY40is anthocyanin accumulation MdMYB15L MdCBF2 cold tolerance

CHH methylation of genes associated with fatty acid and jasmonate biosynthesis contributes to cold tolerance in autotetraploids of Poncirus trifoliata

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Journal of Integrative Plant Biology 2022年第12期64卷 2327-2343页

作者： Yue Wang Lanlan Zuo Tonglu Wei Yu Zhang Yang Zhang Ruhong Ming Dahro Bachar Wei Xiao Khan Madiha Chuanwu Chen Qijun Fan Chunlong Li Ji-Hong Liu Key Laboratory of Horticultural Plant Biology(MOE) College of Horticulture and Foresty SciencesHuazhong Agricultural UniversityWuhan 430070China Guangxi Key Laboratory of Citrus Biology Guangxi Academy of Specialty CropsGuilin 541004China

Polyploids have elevated stress tolerance,but the underlying mechanisms remain largely *** this study,we showed that naturally occurring tetraploid plants of trifoliate orange(Poncirus trifoliata(L.) Raf.) exhibited enhanced cold tolerance relative to their diploid *** analysis revealed that whole-genome duplication was associated with higher expression levels of a range of well-characterized cold stress-responsive *** DNA methylation profiling demonstrated that the tetraploids underwent more extensive DNA demethylation in comparison with the diploids under cold *** methylation in the promoters was associated with up-regulation of related genes,whereas CG,CHG,and CHH methylation in the 3’-regions was relevant to gene *** note,genes involved in unsaturated fatty acids(UFAs) and jasmonate(JA)biosynthesis in the tetraploids displayed different CHH methylation in the gene flanking regions and were prominently up-regulated,consistent with greater accumulation of UFAs and JA when exposed to the cold ***,our findings explored the difference in cold stress response between diploids and tetraploids at both transcriptional and epigenetic levels,and gained new insight into the molecular mechanisms underlying enhanced cold tolerance of the *** results contribute to uncovering a novel regulatory role of DNA methylation in better cold tolerance of polyploids.

关键词： autotetraploid cold tolerance DNA methylation JA Poncirus trifoliata unsaturated fatty acid

Population genomics reveals that natural variation in PRDM16 contributes to cold tolerance in domestic cattle

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Zoological Research 2022年第2期43卷 275-284页

作者： Chun-Long Yan Jun Lin Yuan-Yuan Huang Qing-Shan Gao Zheng-Yu Piao Shou-Li Yuan Li Chen Xue Ren Rong-Cai Ye Meng Dong Han-Lin Zhang Hui-Qiao Zhou Xiao-Xiao Jiang Wan-Zhu Jin Xu-Ming Zhou Chang-Guo Yan College of Agriculture Yanbian UniversityYanjiJilin 133000China Key Laboratory of Animal Ecology and Conservation Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China North-East cold Region Beef Cattle Science&Technology Innovation Ministry of Education Engineering Research Center Yanbian UniversityYanjiJilin 133000China Annoroad Gene Technology Co.Ltd Beijing 100176China

Environmental temperature serves as a major driver of adaptive changes in wild *** discover the mechanisms underpinning cold tolerance in domestic animals,we sequenced the genomes of 28 cattle from warm and cold areas across *** characterizing the population structure and demographic history,we identified two genetic clusters,i.e.,northern and southern groups,as well as a common historic population peak at 30 kilo years *** scan of cold-tolerant breeds determined potential candidate genes in the thermogenesis-related pathways that were under ***,functional analysis identified a substitution of PRDM16(p.P779 L)in northern cattle,which maintains brown adipocyte formation by boosting thermogenesis-related gene expression,indicating a vital role of this gene in cold *** findings provide a basis for genetic variation in domestic cattle shaped by environmental temperature and highlight the role of reverse mutation in livestock species.

关键词： Population genomics Cattle cold tolerance PRDM16 Brown adipose tissue

A systematic identification of cold tolerance genes in peanut using yeast functional screening system

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Oil Crop Science 2023年第3期8卷 184-190页

作者： Xin Wang Yue Liu Ruonan Yao Lei Ouyang Tingting Yu Liying Yan Yuning Chen Dongxin Huai Zhihui Wang Yanping Kang Qianqian Wang Huifang Jiang Yong Lei Boshou Liao Key Laboratory of Biology and Genetic Improvement of Oil Crops Ministry of Agriculture and Rural AffairsOil Crops Research Institute of the Chinese Academy of Agricultural SciencesWuhan430062PR China State Key Laboratory of Biocatalysis and Enzyme Engineering School of Life SciencesHubei UniversityWuhan430062PR China

Peanut(Arachis hypogaea L.)is a thermophilic crop,and low temperature leads to a significant reduction in annual *** a few cold tolerant germplasms or cultivars have been discovered and developed,molecular mechanisms governing peanut cold tolerance is poorly *** of keys genes involved in cold tolerance is the first step to address the underlying *** this study,we isolated and characterized 157 genes with potentials to confer cold tolerance in peanut by using a yeast functional screening ***(Gene ontology)and KEGG(Kyoto encyclopedia of genes and genomes)enrichment analysis of these genes revealed that ribosome and photosynthesis proteins might play essential roles in peanut cold *** results indicated that 60 cold tolerance candidate genes were significantly induced or depressed by low ***-PCR analysis demonstrated that several candidate genes could be also regulated by salt or drought *** overexpression of two UDP-glycosyltransferases(AhUGT2 and AhUGT268)in transgenic yeast cells could enhance their tolerance to multiple abiotic *** conclusion,this study advances our understanding of the mechanisms associated with the cold stress responses in peanut,and offers valuable gene resources for genetic improvement of abiotic stress tolerance in crops.

关键词： Peanut Yeast screening system cold tolerance UDP-glycosyltransferase Abiotic stress

cold tolerance of subtropical Porites lutea from the northern South China Sea

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Acta Oceanologica Sinica 2016年第12期35卷 58-64页

作者： CHEN Tianran LI Shu SHI Qi CHEN Tegu Key Laboratory of Marginal Sea Geology South China Sea Institute of OceanologyChinese Academy of Sciences

Marginal scleractinian corals growing at their latitudinal limits should be quite sensitive to variations in winter sea surface temperatures(SSTs). An extreme cold event occurring in early 2008 offered a unique opportunity to examine the effect of cold-water anomalies on Porites lutea corals and their physiological tolerance and acclimation in the subtropical northern South China Sea(NSCS). Besides in-situ observation, a subsequent aquarium-based experiment was designed for reproducing the chilling process and a 50-year-long Sr/Ca ratio profile from two P. lutea skeletal slabs was analyzed for reconstructed the historical annual minimum SSTs which ceased Porites calcification. The 2008 low-temperature anomaly caused the minimum daily mean SSTs dropped below 13°C in the Daya Bay. The stress symptoms displayed by local P. lutea colonies included polyp retraction, reduced coloration and pale, but none showed tissue sloughing. The ability of P. lutea to survive implied its tolerance of extreme low temperatures. Here we suggest a model on the tolerance of high-latitude Porites under low-temperature stresses, which is when SSTs drop below 18°C, Porites corals contract their tentacles(losing heterotrophic capability), then cease calcification(reducing energy consumption), and meanwhile maintain relatively high levels of zooxanthellae density(sustaining host’s life via photosynthetic capacity of symbiotic zooxanthellae). This study revealed remarkable acclimatization of P. lutea corals to low temperature extremes. This acclimatization is beneficial for Porites corals in the NSCS to expand their living ranges towards the higher-latitude areas and have the potential to be the incipient reef former.

关键词： low-temperature stress cold tolerance subtropical coral Porites lutea northern South China Sea

Linkage map construction and QTL mapping for cold tolerance in Oryza rufipogon Griff. at early seedling stage

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Journal of Integrative Agriculture 2016年第12期15卷 2703-2711页

作者： LUO Xiang-dong ZHAO Jun DAI Liang-fang ZHANG Fan-tao ZHOU Yi WAN Yong XIE Jian-kun College of Life Science Jiangxi Normal University Rice Research Institute Jiangxi Academy of Agricultural Science

cold stress is one of the major restraints for rice production. cold tolerance is controlled by complex genetic factor. In this study, a backcross inbred lines （BILs） population derived from an inter-specific cross （Oryza sativa ***. rufipogon Griff.） was used for genetic linkage map construction and quantitative trait locus （QTL） mapping. A linkage map consisting of 153 markers was constructed, spanning 1 596.8 cM with an average distance of 11.32 cM between the adjacent markers. Phenotypic evaluation of the parents and BILs under （6±1）℃ cold stress revealed that the ability of cold tolerance in BILs at early seedling obeyed a skewed normal and continuous distribution. Fifteen QTLs on chromosomes 6, 7, 8, 11, and 12 were identified using survival percent （SP） and non death percent （NDP） as indicators of cold tolerance, which could explain 5.99 to 40.07% of the phenotypic variance, of which the LOD values ranged from 3.04 to 11.32. Four QTLs on chromosomes 3, 5 and 7 were detected using leaf conductivity （LC） and root conductivity （RC） as indicators of cold tolerance, ranging from 19.54 to 33.53% for the phenotypic variance explained and 2.54 to 6.12 for the LOD values. These results suggested that there might be multi major QTLs in O. rufipogon and some useful genes for cold tolerance have been transferred into cultivated rice, which would be helpful for cloning and utilizing the cold tolerance-responsive genes from wild rice.

关键词： common wild rice cold tolerance quantitative trait loci (QTL) introgression