Genetic variation in a heat shock transcription factor modulates cold tolerance in maize
作者机构：State Key Laboratory of Plant Environmental ResilienceFrontiers Science Center for Molecular Design BreedingCollege of Biological SciencesCenter for Crop Functional Genomics and Molecular BreedingChina Agricultural UniversityBeijing 100193China State Key Laboratory of Maize Bio-breedingNational Maize Improvement CenterFrontiers Science Center for Molecular Design BreedingDepartment of Plant Geneticsand BreedingChinaAgricultural UniversityBeijingChina State Key Laboratory of Plant Genomics and National Plant Gene Research CenterInstitute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China
出 版 物：《Molecular Plant》 (分子植物（英文版）)
年 卷 期：2024年第17卷第9期
页 面：1423-1438页
核心收录：
基 金：supported by Biological Breeding-National Science and Technology Major Project of China(2023ZD0407104) the National Natural Science Foundation of China(32272025 and 31730011) the Pinduoduo-China Agricultural University Research Fund(PC2023B01001),and the Chinese Universities Scientific Fund
主 题：heat shock factor HSF21 cold tolerance natural variation bZiP68 lipid metabolism maize
摘 要：Understanding how maize(Zea mays)responds to cold stress is crucial for facilitating breeding programs of cold-tolerant *** extensive utilization of the genome-wide association study(GWAs)approach for exploring favorable natural alleles associated with maize cold tolerance,few studies have successfully identified candidate genes that contribute to maize cold *** this study,we used a diverse panel of inbred maize lines collected from different germplasm sources to perform a GWAS on var-iations in the relative injured area of maize true leaves during cold stress-a trait very closely correlated with maize cold *** identified HsF21,which encodes a B-class heat shock transcription factor(HSF)that positively regulates cold tolerance at both the seedling and germination *** varia-tions in the promoter of the cold-tolerant HSF21Hap1 allele led to increased HSF21 expression under cold stress by inhibiting binding of the basic leucine zipper bziP68 transcription factor,a negative regulator of cold *** integrating transcriptome deep sequencing,DNA affinity purification sequencing,and targeted lipidomic analysis,we revealed the function of HsF21 in regulating lipid metabolism homeo-stasis to modulate cold tolerance in *** addition,we found that HsF21 confers maize cold tolerance without incurring yield ***,this study establishes HsF21 as a key regulator that en-hances cold tolerance in maize,providing valuable genetic resources for breeding of cold-tolerant maize varieties.
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