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4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone Induces circulating microrna Deregulation in Early Lung Carcinogenesis

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Biomedical and Environmental Sciences 2014年第1期27卷 10-16页

作者： WU Jian Jun YANG Ti LI Xun XIA Yuan ZHAO Yao ZOU Fei JIANG Yi Guo School of Public Health and Tropical Medicine Southern Medical University Institute for Chemical Carcinogenesis State Key Laboratory of Respiratory DiseaseGuangzhou Medical University School of Public Health Guangdong Pharmaceutical University

Objective To study the alteration of circulating micrornas in 4-（methylnitrosamino）-l-（3-pyridyl） -l-butanone （NNK）-induced early stage lung carcinogenesis. Methods A lung cancer model of male F344 rats was induced with systemic NNK and levels of 8 lung cancer-associated miRNAs in whole blood and serum of rats were measured by quantitative RT-PCR of each at weeks 2, 5, 20, and 20 following NNK treatment. Results No lung cancer was detected in control group and NNK treatment group at week 20 following NNK treatment. The levels of some circulating miRNAs were significantly higher in NNK treatment group than in control group. The miR-210 was down-regulated and the miR-206 was up-regulated in NNK treatment group. The expression level of circulating miRNAs changed from week 1 to week 20 following NNK treatment. Conclusion The expression level of circulating miRNAs is related to NNK-induced early stage lung carcinogenesis in rats and can therefore serve as its potential indicator.

关键词： NNK circulating microrna Early stage Lung carcinogenesis

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circulating micrornas: Novel biomarkers for esophageal cancer

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World Journal of Gastroenterology 2010年第19期16卷 2348-2354页

作者： Zhou, Sheng-Li Wang, Li-Dong Xinxiang Med Univ Canc Res Ctr Xinxiang 453003 Henan Peoples R China Zhengzhou Univ Basic Med Coll Henan Key Lab Esophageal Canc Res Zhengzhou 450052 Henan Peoples R China

Esophageal carcinogenesis is a multi-stage process, involving a variety of changes in gene expression and physiological structure change. micrornas (miRNAs) are a class of small non-coding endogenous RNA molecules. Recent innovation in miRNAs profiling technology have shed new light on the pathology of esophageal carcinoma (EC), and also heralded great potential for exploring novel biomarkers for both EC diagnosis and treatment. Frequent dysregulation of miRNA in malignancy highlights the study of molecular factors upstream of gene expression following the extensive investigation on elucidating the important role of miRNA in carcinogenesis. We herein present a thorough review of the role of miRNAs in EC, addressing miRNA functions, their putative role as oncogenes or tumor suppressors and their potential target genes. The recent progresses in discovering the quantifiable circulating cancer-associated miRNAs indicate the potential clinical use of miRNAs as novel minimally invasive biomarkers for EC and other cancers. We also discuss the potential role of miRNAs in detection, screening and surveillance of EC as miRNAs can be a potential target in personalized treatment of EC.

关键词： Esophageal cancer circulating microrna

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circulating micrornas as a liquid biopsy: a next generation clinical biomarker for diagnosis of gastric cancer

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Journal of Cancer Metastasis and Treatment 2018年第1期4卷 418-427页

作者： Shuhei Komatsu Jun Kiuchi Taisuke Imamura Daisuke Ichikawa Eigo Otsuji Division of Digestive Surgery Department of SurgeryKyoto Prefectural University of MedicineKawaramachihirokojiKamigyo-kuKyoto 602-8566Japan First Department of Surgery Faculty of MedicineUniversity of YamanashiChuoYamanashi 409-3898Japan

Accumulating evidence has suggested the potential clinical utility of novel body fluid biomarkers,or“liquid biopsy”,using circulating tumor cells and cell-free nucleic acids from cancer *** and reproducible,liquid biopsy could provide the basis for individualized therapeutic strategies by identifying genetic and epigenetic aberrations that are closely associated with cancer initiation and ***(miRNAs)are short noncoding RNAs that post-transcriptionally regulate gene *** also play important roles in various physiological and developmental processes as oncogenic or tumor-suppressive *** miRNA expression signatures have been identified in a number of human *** miRNAs have been detected in plasma and serum,and this in blood has attracted the attention of researchers for their potential as noninvasive *** miRNAs have emerged as tumor-associated biomarkers that reflect not only the existence of cancer,but also the dynamics,malignant potential,and drug resistance of ***,we review the recent biological and clinical research on the circulating miRNAs of gastric cancer and discuss future perspectives for their clinical applications as a liquid biopsy.

关键词： Liquid biopsy circulating nucleic acids circulating microrna biomarker gastric cancer

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Serum outperforms plasma in small extracellular vesicle microrna biomarker studies of adenocarcinoma of the esophagus

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World Journal of Gastroenterology 2020年第20期26卷 2570-2583页

作者： Karen Chiam George C Mayne Tingting Wang David I Watson Tanya S Irvine Tim Bright Lorelle T Smith Imogen A Ball Joanne M Bowen Dorothy M Keefe Sarah K Thompson Damian J Hussey Discipline of Surgery College of Medicine and Public HealthFlinders University of South AustraliaAdelaideSA 5042Australia Flinders Health and Medical Research Institute Cancer Program College of Medicine and Public HealthFlinders UniversityAdelaideSA 5042Australia Discipline of Surgery The University of AdelaideAdelaideSA 5005Australia Adelaide Medical School The University of AdelaideAdelaideSA 5005Australia Faculty of Health Sciences The University of AdelaideAdelaideSA 5005Australia

BACKGROUND circulating micrornas(miRNAs)are potential biomarkers for many ***,they can originate from non-disease specific sources,such as blood cells,and compromise the investigations for miRNA *** small extracellular vesicles(sEVs)have been suggested to provide a purer source of circulating miRNAs for biomarkers discovery,the most suitable blood sample for sEV miRNA biomarker studies has not been *** To compare the mi RNA profiles between matched serum and plasma s EV preparations to determine their suitability for biomarker *** Matched serum and plasma samples were obtained from 10 healthy controls and10 patients with esophageal adenocarcinoma.s EV isolates were prepared from serum and plasma using Exo Quick TM and quantified using Nano *** was extracted from s EV preparations with the mi RNeasy Serum/Plasma kit and profiled using the Taqman Openarray q *** overall mi RNA content and theexpression of specific mi RNAs of reported vesicular and non-vesicular origins were compared between serum and plasma s EV *** diagnostic performance of a previously identified multi-mi RNA biomarker panel for esophageal adenocarcinoma was also *** The overall mi RNA content was higher in plasma s EV preparations(480 mi RNAs)and contained 97.5%of the mi RNAs found in the serum s EV preparations(412 mi RNAs).The expression of commonly expressed mi RNAs was highly correlated(Spearman’s R=0.87,P<0.0001)between the plasma and serum s EV preparations,but was consistently higher in the plasma s EV *** blood-cell mi RNAs(hsa-mi R-223-3 p,hsa-mi R-451 a,mi R-19 b-3 p,hsa-mi R-17-5 p,hsa-mi R-30 b-5 p,hsa-mi R-106 a-5 p,hsa-mi R-150-5 p and hsa-mi R-92 a-3 p)were expressed at 2.7 to 9.6 fold higher levels in the plasma s EV preparations compared to serum s EV preparations(P<0.05).In plasma s EV preparations,the percentage of protein-associated mi RNAs expressed at relatively higher levels(Ct

关键词： Biomarkers Exosomes Extracellular vesicles circulating microrna micrornas Plasma Serum Blood cells Real-time polymerase chain reaction Adenocarcinoma of esophagus

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Macronutrient modulation of mRNA and microrna function in animals:A review

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Animal Nutrition 2020年第3期6卷 258-268页

作者： Md Mahmodul Hasan Sohel Department of Genetics Faculty of Veterinary MedicineErciyes University Genome and Stem Cell Centre Erciyes University

Dietary macronutrients have been regarded as a basic source of energy and amino acids that are necessary for the maintenance of cellular homeostasis,metabolic programming as well as protein *** to the emergence of"nutrigenomics",a unique discipline that combines nutritional and omics technologies to study the impacts of nutrition on genomics,it is increasingly evident that macro nutrients also have a significant role in the gene expression *** expression is a complex phenomenon controlled by several signaling pathways and could be influenced by a wide variety of environmental and physiological *** macronutrients are the most important environmental factor influencing the expression of both genes and micrornas(miRNA).miRNA are tiny molecules of 18 to 22 nucleotides long that regulate the expression of ***,dietary macro nutrients can influence the expression of genes in both direct and indirect *** advancements in the state-of-the-art technologies regarding molecular genetics,such as next-generation sequencing,quantitative PCR array,and microarray,allowed us to investigate the occurrence of genome-wide changes in the expression of genes in relation to augmented or reduced dietary macronutrient intake,The purpose of this review is to accumulate the current knowledge focusing on macronutrient mediated changes in the gene *** review will discuss the impact of altered dietary carbohydrate,protein,and fat intake on the expression of coding genes and their *** addition,it will also summarize the regulation of miRNA,both cellular and extracellular miRNA,expression modulated by dietary macronutrients.

关键词： Nutrigenomics microrna circulating microrna Carbohydrate Protein Fat

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