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Precise capture of circulating endometrial cells in endometriosis

中华医学杂志(英文版) 2024年第14期137卷 1715-1723页

作者： Wang Shang Cheng Hongyan Zhu Honglan Yu Xiaoming Ye Xue Chang Xiaohong Department of Obstetrics and Gynecology Peking University People’s Hospital Beijing China Reproductive Medicine Center Peking University People’s Hospital Beijing China

Background: Endometriosis (EM) is a complex benign gynecological disease, but it has malignant biological behavior and can invade any part of the body. Clinical manifestations include pelvic pain, dysmenorrhea, infertility, pelvic nodules, and masses. Our previous study successfully detected circulating endometrial cells (CECs) in the peripheral blood of patients with EM. The purpose of this study is to overcome the limitation of cell size in the previous microfluidic chip method, to further accurately capture CECs, understand the characteristics of these cells, and explore the relationship between CECs and the clinical course characteristics of patients with ***: Human peripheral venous blood used to detect CECs and circulating vascular endothelial cells (CVECs) was taken from EM patients (n = 34) hospitalized in the Peking University People’s Hospital. We used the subtraction enrichment and immunostaining fluorescencein situ hybridization (SE-iFISH) method to exclude the interference of red blood cells, white blood cells, and CVECs, so as to accurately capture the CECs in the peripheral blood of patients with EM. Then we clarified the size and ploidy number of chromosome 8 of CECs, and a second grouping of patients was performed based on clinical characteristics to determine the relationship between CECs and clinical course ***: The peripheral blood of 34 EM patients and 12 non-EM patients was evaluated by SE-iFISH. Overall, 34 eligible EM patients were enrolled. The results showed that the detection rates of CECs were 58.8% in EM patients and 16.7% in the control group. However, after classification according to clinical characteristics, more CECs could be detected in the peripheral blood of patients with rapidly progressive EM, with a detection rate of 94.4% (17/18). In total, 63.5% (40/63) of these cells were small cells with diameters below 5 μm, and 44.4% (28/63) were aneuploid cells. No significant association was found between the

关键词： Endometriosis circulating endometrial cells Clinical symptoms Dysmenorrhea circulating vascular endothelial cells

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Evaluation of circulating endometrial cells as a Biomarker for Endometriosis

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Chinese Medical Journal 2017年第19期130卷 2339-2345页

作者： Ying Chen Hong-Lan Zhu Zhe-Wen Tang Kuang Hong Neoh Dong-Fang Ouyang Heng Cui Hong-Yan Cheng Rui-Qiong Ma Xue Ye Ray P. S. Han Xiao-Hong Chang Gynecological Oncology Center Peking University People's Hospital Beijing 100044 China Department of Materials Science and Engineering Peking University Beijing 100871 China Department of Mechanical and Industrial Engineering University of Toronto Toronto Ontario M5S 3G8 Canada

Background： circulating endometrial cells （CECs） have been reported to be present in the peripheral blood of women with endometriosis （EM）, providing clear and specific evidence of the presence of ectopic lesions. In this study, we established a method with a high detection rate of CECs, assessed the diagnostic value of CECs for EM and compared with serum CA125, and proposed a hypothesis for the pathogenesis of EM from the new perspective of CECs. Methods： The participants were enrolled prospectively from October 2015 to July 2016. The peripheral blood samples were collected from 59 participants, and the blood cells were isolated for immunofluorescence staining via microfluidic chips. The cells that were positive for vimentin/cytokeratin and estrogen/progesterone receptor and negative for CD45 were identified as CECs. The serum CA125 level was tested with electrochemiluminescence immunoassay. Results： The detection rate of CECs reached 89.5% （17/19） in the EM group, which was significantly higher than that of the control group （15.0% [6/40], P 〈 0.001） and was independent of menstrual cycle phases. Furthermore, a positive CEC assay detected 4/5 cases of Stage Ⅰ–Ⅱ EM. In contrast, a positive CA125 test had limited value in detecting EM （13/19, 68.4%） and detected only one case of Stage Ⅰ–Ⅱ EM. Conclusion： CECs are promising biomarkers for EM with great potential for a noninvasive diagnostic assay.

关键词： Biomarker circulating endometrial cells Clinical Diagnosis Endometriosis Pathogenesis

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