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PCR analysis of yq microdeletions in infertile males, a study from South India

Asian Journal of Andrology 2002年第4期4卷 265-268页

作者： S. Ramesh Babu M. Swarna P. Padmavathi P.P. Reddy Institute of Genetics & Hospital for Genetic Diseases Scientist Osmania University Begumpet Hyderabad-500 016 IndiaInstitute of Genetics & Hospital for Genetic Diseases Scientist Osmania University Begumpet Hyderabad-500 016 IndiaInstitute of Genetics & Hospital for Genetic Diseases Scientist Osmania University Begumpet Hyderabad-500 016 IndiaInstitute of Genetics & Hospital for Genetic Diseases Scientist Osmania University Begumpet Hyderabad-500 016 India

AIM: To estimate the frequency of microdeletions in the long arm of y-chromosome of 20 infertile males from South India. METHODS: Polymerase chain reaction (PCR) amplification using y-specific STS of azoospermia factor (AZF) regions i.e., Sy 84 for AZFa, Sy 127 for AZFb and Sy 254 for AZFc. RESULTS: Of the 20 infertile subjects 3 (15 %), one azoospermic and two oligozoospermic, showed microdeletions in the AZF region of y-chromosome. CONCLUSION: The frequency of deletions involving AZF region of the y-chromosome is 15 % in azoospermic and severely oligozoospermic infertile men. PCR amplification of AZF locus is useful for the diagnosis of microdeletions in the y-chromosome.

关键词： Chromosome Deletion chromosomes, human, y Base Sequence Chromosome Mapping Comparative Study DNA Primers Female Gene Frequency humans India Infertility, Male Male Oligospermia Polymerase Chain Reaction Reference Values Research Support, Non-U.S. Gov't Seminal Plasma Proteins

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Lack of evidence for leukocyte maternal microchimerism in primary biliary cirrhosis

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World Journal of Gastroenterology 2004年第16期10卷 2415-2416页

作者： Kenichi Nomura yoshio Sumida Takaharu yoh Atsuhiro Morita yosuke Matsumoto SawakoTaji Naohisayoshida MasahitoMinami yoshitoItoh ShigeoHoriike Keisho Kataoka Masafumi Taniwaki Takeshi Okanoue Molecular Hematology and Oncology Kyoto Prefectural University of Medicine Graduate School of Medical ScienceKyoto 602-0841Japan Department of Internal Medicine National Nara HospitalNara 630-8305Japan Department of Internal Medicine Aiseikai Yamashina HospitalKyoto 607-8086Japan Ayabe city Hospital Kyoto 623-0011Japan Molecular Gastroenterology and Hepatology Kyoto Prefectural University of Medicine Graduate School of Medical ScienceKyoto 602-0841Japan Clinical Molecular Genetics and Laboratory Medicine Kyoto Prefectural University of Medicine Graduate School of Medical ScienceKyoto 602-0841Japan

AIM:It is reasonable to assume that microchimerism could also be involved in the induction of primary biliary cirrhosis (PBC).However,previous reports investigated only fetus-microchimerism in women *** microchimerism has not been investigated until now. The current study aimed to clear either maternal microchimerism was involved in the pathogenesis of PBC or not. METHODS:We used fluorescence in situ hybridization on paraffin-embedded tissue (We called“Tissue-FiSH”.) to determine whether maternal cells infiltrated in male patients who were diagnosed as having ***-FiSH was performed by using both X and y specific probes on the biopsy liver sample of 3 male PBC patients. RESULTS:Infiltrating lymphocytes demonstrated both X and y signals in all 3 male patients. CONCLUSION:Maternal microchimerism dose not play a significant role in *** may not relate to fetus and maternal microchimerism.

关键词： Chimera chromosomes, human, X chromosomes, human, y Female humans In Situ Hybridization, Fluorescence Leukocytes Liver Liver Cirrhosis, Biliary Lymphocytes Male Research Support, Non-U.S. Gov't

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