PCR analysis of Yq microdeletions in infertile males, a study from South India

作     者：S. Ramesh Babu M. Swarna P. Padmavathi P.P. Reddy S. RAMESH BABU;M. SWARNA;P. PADMAVATHI;P.P. REDDY ~INSTITUTE OF GENETICS & HOSPITAL FOR GENETIC DISEASES;SCIENTIST;OSMANIA UNIVERSITY;BEGUMPET;HYDERABAD-;INDIA

作者机构：Institute of Genetics & Hospital for Genetic Diseases Scientist Osmania University Begumpet Hyderabad-500 016 IndiaInstitute of Genetics & Hospital for Genetic Diseases Scientist Osmania University Begumpet Hyderabad-500 016 IndiaInstitute of Genetics & Hospital for Genetic Diseases Scientist Osmania University Begumpet Hyderabad-500 016 IndiaInstitute of Genetics & Hospital for Genetic Diseases Scientist Osmania University Begumpet Hyderabad-500 016 India 

出 版 物：《Asian Journal of Andrology》 (亚洲男性学杂志（英文版）)

年 卷 期：2002年第4卷第4期

页      面：265-268页

核心收录：

学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学] 

主　　题：Chromosome Deletion Chromosomes, Human, Y Base Sequence Chromosome Mapping Comparative Study DNA Primers Female Gene Frequency Humans India Infertility, Male Male Oligospermia Polymerase Chain Reaction Reference Values Research Support, Non-U.S. Gov't Seminal Plasma Proteins 

摘      要：AIM: To estimate the frequency of microdeletions in the long arm of Y-chromosome of 20 infertile males from South India. METHODS: Polymerase chain reaction (PCR) amplification using Y-specific STS of azoospermia factor (AZF) regions i.e., SY 84 for AZFa, SY 127 for AZFb and SY 254 for AZFc. RESULTS: Of the 20 infertile subjects 3 (15 %), one azoospermic and two oligozoospermic, showed microdeletions in the AZF region of Y-chromosome. CONCLUSION: The frequency of deletions involving AZF region of the Y-chromosome is 15 % in azoospermic and severely oligozoospermic infertile men. PCR amplification of AZF locus is useful for the diagnosis of microdeletions in the Y-chromosome.