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Transcriptome-based insights into the calcium transport mechanism of chick chorioallantoic membrane

Food Science and Human Wellness 2022年第2期11卷 383-392页

作者： Qun Huang Ran Yang Qia Wang Hui Teng Hongbo Song Fang Geng Peng Luo School of Public Health The Key Laboratory of Environmental Pollution Monitoring and Disease ControlMinistry of EducationGuizhou Medical UniversityGuiyang 550025China College of Food Science Fujian Agriculture and Forestry UniversityFuzhou 350002China Meat Processing Key Laboratory of Sichuan Province School of Food and Biological EngineeringChengdu UniversityChengduSichuan 610106China

chorioallantoic membrane(CAM)is responsible for respiratory gas exchange,eggshell calcium transport,embryonic acid-base equilibrium,allantoic ion,and water reabsorption during avian embryonic *** further understand the timing of CAM gene expression during chick embryonic development,especially the calcium absorption mechanism,transcriptome quantitative comparative analysis was conducted on chick CAM during the embryonic period(E)of 9,13,17,and 20 days.A total of 6378 differentially expressed genes(DEGs)were *** enrichment analysis of DEGs showed that CAM DEGs were mainly involved in biological processes such as"ion transport regulation","immune response"and"cell cycle".Time series analysis of the differential genes showed that the functional cells of CAM began to proliferate and differentiate at E9 and the calcium content of egg embryo increased significantly at ***,the observation of the ultrastructure of the eggshell showed that the interstice of the fiber layer was enlarged at E13,and the mastoid layer was partly ***,it is preliminarily inferred that CAM calcium transport starts at E13,and genes such as TRPV6,S100 A10,and RANKL cooperate to regulate calcium release and transport.

关键词： Incubation chorioallantoic membrane Transcriptome Calcium transport

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Establishment of xenografts of urological cancers on chicken chorioallantoic membrane(CAM)to study metastasis

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Precision Clinical Medicine 2019年第3期2卷 140-151页

作者： Junhui Hu Moe Ishihara Arnold IChin Lily Wu Department of Molecular and Medical Pharmacology David Geffen School of MedicineUniversity of California at Los AngelesCA 90095USA Department of Urology David Geffen School of MedicineUniversity of California at Los AngelesCA 90095USA Department of Pediatrics David Geffen School of MedicineUniversity of California at Los AngelesCA 90095USA Jonsson Comprehensive Cancer Center David Geffen School of MedicineUniversity of California at Los AngelesCA 90095USA

Cancer of the urological system commonly occurs in the kidney,bladder,and prostate *** clear cell subtype of renal cell carcinoma(ccRCC)constitutes the great majority of kidney *** ccRCC portends a very poor outcome with no effective treatment *** cancer is the most common cancer in males in the *** recent advances in selective kinase inhibitors and immunotherapies,the rate of developing new treatment from bench to bedside is slow.A time-consuming step is at the animal drug testing stage,in which the mouse model is the gold *** the pursuit to streamline the in vivo cancer biology research and drug development,we explored the feasibility of the chicken chorioallantoic membrane(CAM)model to establish *** CAM model greatly shortens the time of tumor growth and lowers the cost comparing to immunocompromised *** generated CAM xenografts from ccRCC,bladder and prostate cancer,with established cancer cell lines and freshly isolated patient-derived tissues,either as primary tumor cells or small pieces of *** successful CAM engraftment rate from the different tumor sources is 70%or *** our previously established metastatic ccRCC mouse model,we showed that the CAM xenograft maintains the same tumor growth pattern and metastatic behavior as observed in *** together,CAM can serve as a valuable platform to establish new patient-derived xenografts(PDXs)to study tumor biology,thus accelerating the development of individualized treatment to halt the deadly metastatic stage of cancer.

关键词： chorioallantoic membrane prostate cancer bladder cancer kidney cancer animal mode

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Saikosaponins-b suppresses tumor growth and angiogenesis of hepatocellular carcinoma by regulating VEGF/ERK/HIF-1α signal pathway

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中国药理学与毒理学杂志 2017年第10期31卷 962-963页

作者： Rui-fang LI Jun-min FU Xing-zhi LYU Zi-han GAO Hong-wei WANG Jian-gang WANG Laboratory of Pharmacology and Molecular Biology Medical CollegeHenan University of Science and Technology School of Nursing Henan University of Science and Technology

OBJECTIVE Angiogenesis therapy has attracted interest as a potential treatment for hepatocellular carcinoma(HCC).In this study,we investigated the anti-proliferative activities and antiangiogenesis effects of saikosaponins(SS)-b on hepatocellular carcinoma(HCC)and its regulation on VEGF/ERK/HIF-1 αsignal *** H22 hepatoma-bearing mice model and HepG-2 cells were used to study the anti-tumor and anti-angiogenesis effects of SS-b in vivo and in *** change of tumor tissue was observed by HE staining,the microvascular changes were detected by immunohistochemical *** effects of SS-b on angiogenesis were examined by using the chick embryo chorioallantoic membrane(CAM)*** effects of SS-b on proliferation,migration and invasion were investigated by MTT assay,scratch wound healing assay and transwell assay inhuman umbilical vein endothelial cell(HUVEC)and HepG2 cells in *** endothelial growth factor(VEGF),matrix metalloproteinase-2/9(MMP-2/9),hypoxia-inducible factor-1α(HIF-1α)expression and the phosphorylation of extracellular regulated kinase(ERK)were analyzed using RT-PCR and *** SS-b effectively inhibited the tumor growth of H22 mice in *** inhibitory rate of tumor was 49.1%,50.7%,66.1%in SS-b 5,10 and 20 mg·kg-1group *** staining results showed that SS-b induced tumor necrosis and nuclear dissolution in H22 ***,SS-b also reduced the number of microvessels of tumor tissue in H22 mice significantly and suppressed the angiogenesis of CAM induced by ***-b had an obvious inhibitory effect on cell proliferation,migration and invasion of HUVEC cells and HepG-2 *** effects were associated with downregulation of the expression of MMP2/9 and suppression of VEGF/ERK/HIF-1αsignaling in H22 mice and Hep-G2 *** Our findings showed that SS-b exerts anti-tumor effects by inhibiting tumor angiogenesis via regulating VEGF/ERK/HIF-1α signal pathway in vivo and in vitro.

关键词： saikosaponins-b angiogenesis hepatocellular carcinoma chorioallantoic membrane HUVEC cells

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Anti-Angiogenesis and Anti-Tumor Effect of Shark Cartilage Extract

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Tsinghua Science and Technology 2001年第3期6卷 200-205,247页

作者：王锋王漪涛谢莉萍张荣庆 Department of Biological Sciences and Biotechnology Center for Ocean Science and Engineering' Tsinghua University Beijing 100084 China

The effect of shark cartilage extract (SCE), purified in this laboratory, on angiogenesis in chick chorioallantoic membrane (CAM), on the activity of collagenase IV and on human umbilical vein endothelial cell (ECV 304) proliferation and apoptosis was investigated in vitro. The results showed that SCE caused a decline in CAM blood vessels and significantly prevented collagenase induced collagenolysis. Moreover, SCE produced a dose dependent decline in ECV 304 proliferation and altered its normal cell cycle. These results suggest that the anti angiogenesis and anti tumor effects of shark cartilage may be due to inhibition of endothelial cells as well as collagenolysis. 

关键词： shark cartilage extract angiogenesis chorioallantoic membrane collagenase endothelial cell

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Evaluation,partial characterization and purification of acetylcholine esterase enzyme and antiangiogenic activity from marine sponges

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Journal of Coastal Life Medicine 2014年第11期 849-854页

作者： Maushmi Shailesh Kumar Sukanya Gopalkrishnan Department of Pharmaceutical Biotechnology Shobhaben Pratapbhai Patel School of Pharmacy and Technology ManagementSVKM’S NMIMSVile Parle westMumbai-400056India

Objective:To test three marine sponges Halichondria glabrata Keller,1891;Spirastrellapachyspira(***)Levi,1958 and Cliona lobata Hancock,1849 for the presence of the acetylcholinesterase(AChE)in both young and developed samples from western coastal area of *** methanolic extract was selected for anti/pro angiogenic ***:They were evaluated for AChE activity using Ellman’s assay based on production of yellow colored *** of the enzyme was planned using ammonium sulphate precipitation and characterization by sodium dodecyl sulfate polyacrylamide gel *** membrane(ChAM)assay model was used for angiogenic/antiangiogenic ***:All the three sponges showed good specific enzyme activity and *** contained maximum specific enzyme *** percent of ammonium sulphate precipitation of crude protein sample gave single band at 66 kDa corresponding to the true *** assay was performed at 62.5,125.0 and 250.0μg/*** beyond 250μg/mL extract showed toxic response with anti angiogenic activity at all the ***:AChE activity was detected in all *** showed good anti-angiogenic response at 62.5μg/*** was highly toxic affecting microvasculature of ChAM as well as normal growth and development of the embryo at 500μg/*** further characterization of bioactive compounds from the extract of ***,the compounds can be developed for anti tumor activity.

关键词： Halichondria glabrata Keller Spirastrella pachyspira Levi Cliona lobata Hancock Acetylcholinesterase chorioallantoic membrane Antiangiogenic activity Sodium dodecyl sulfate polyacrylamide gel electrophoresis

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