Transcriptome-based insights into the calcium transport mechanism of chick chorioallantoic membrane

作     者：Qun Huang Ran Yang Qia Wang Hui Teng Hongbo Song Fang Geng Peng Luo Qun Huang;Ran Yang;Qia Wang;Hui Teng;Hongbo Song;Fang Geng;Peng Luo

作者机构：School of Public HealthThe Key Laboratory of Environmental Pollution Monitoring and Disease ControlMinistry of EducationGuizhou Medical UniversityGuiyang 550025China College of Food ScienceFujian Agriculture and Forestry UniversityFuzhou 350002China Meat Processing Key Laboratory of Sichuan ProvinceSchool of Food and Biological EngineeringChengdu UniversityChengduSichuan 610106China 

出 版 物：《Food Science and Human Wellness》 (食品科学与人类健康（英文）)

年 卷 期：2022年第11卷第2期

页      面：383-392页

核心收录：

学科分类：0905[农学-畜牧学] 09[农学] 090501[农学-动物遗传育种与繁殖] 

基　　金：financially supported by the Foundation of Guizhou Educational Committee(No.KY008 and No.KY014) the National Natural Science Foundation of China(No.31871732) 

主　　题：Incubation Chorioallantoic membrane Transcriptome Calcium transport 

摘      要：Chorioallantoic membrane(CAM)is responsible for respiratory gas exchange,eggshell calcium transport,embryonic acid-base equilibrium,allantoic ion,and water reabsorption during avian embryonic *** further understand the timing of CAM gene expression during chick embryonic development,especially the calcium absorption mechanism,transcriptome quantitative comparative analysis was conducted on chick CAM during the embryonic period(E)of 9,13,17,and 20 days.A total of 6378 differentially expressed genes(DEGs)were *** enrichment analysis of DEGs showed that CAM DEGs were mainly involved in biological processes such asion transport regulation,immune responseandcell cycle.Time series analysis of the differential genes showed that the functional cells of CAM began to proliferate and differentiate at E9 and the calcium content of egg embryo increased significantly at ***,the observation of the ultrastructure of the eggshell showed that the interstice of the fiber layer was enlarged at E13,and the mastoid layer was partly ***,it is preliminarily inferred that CAM calcium transport starts at E13,and genes such as TRPV6,S100 A10,and RANKL cooperate to regulate calcium release and transport.